1887

Journal of Medical Microbiology logo

Volume 14, Issue 4

Transferable plasmid-mediated antibiotic resistance in Free

Abstract

Summary.

Plasmid-mediated resistance to chloramphenicol (Chl), erythromycin (Ery), tetracycline (Tet) and clindamycin (Clind) was transferred from three clinical isolates of and one faecal isolate of to strains of and , and subsequently to and second-and third-stage recipients in series. Successful transfer was achieved by membrane-filter and centrifugation techniques that provide stable cell-to-cell contact but not by simple mixed broth culture. Chl Ery Tet and Clind Ery were transferred at high frequency (1·9 × 10 –1·8 × 10) but Tet was transferred at low frequencies (1–1·6 × 10). Segregation of resistance markers was observed with selection for Tet when donors were Chl Ery Tet and Chl Tet. All transcipients were identical with the parent recipient strains but had the resistance markers of the donor strains. Resistance to antibiotics other than tetracycline was cured by growth with subinhibitory concentrations of aminoacri-dines and ethidium bromide for 24 h; cure of solitary Tet required longer incubation (21 days). Identical plasmid DNA bands were demonstrated by agarose-gel electrophoresis in all the donor and corresponding transcipient strains but plasmids were not found in the recipient strains or in strains cured of resistance. Plasmid-mediated transferable antiobiotic resistance in spp. may compromise the treatment of infections and may provide a reservoir of antibiotic resistance in the intestinal flora.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1981
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-14-4-359
1981-11-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/14/4/medmicro-14-4-359.html?itemId=/content/journal/jmm/10.1099/00222615-14-4-359&mimeType=html&fmt=ahah

References

  1. Booth S. J., van Tassell R. L., Johnson J. L., Wilkins T. D. 1979; Bacteriophages of bacteroides. Rev. infect. Dis. 1:325
     [Google Scholar]
  2. Brefort G., Magot M., Ionesco H., Sebald M. 1977; Characterisation and transferability of Clostridium perfringens plasmids. Plasmid 1:52
     [Google Scholar]
  3. Burt S. J., Woods D. R. 1976; R factor transfer to obligate anaerobes from Escherichia coli . J. gen. Microbiol. 93:405
     [Google Scholar]
  4. Clarke J. S., Condon R. E., Bartlett J. G., Gorbach S. L., Nichols R. L., Ochi S. 1977; Pre-operative oral antibiotics reduce septic complications of colon operations: results of prospective, randomized, double-blind clinical study. Ann. Surg. 186:251
     [Google Scholar]
  5. Cowan S. T. 1974 Cowan and Steel’s manual for the identification of medical bacteria, 2. Cambridge University Press; Cambridge:
     [Google Scholar]
  6. Datta N., Faiers M. C., Reeves D. S., Brumfitt W., Ørskov F., Ørskov I. 1971; R factors in Escherichia coli in faeces after oral chemotherapy in general practice. Lancet 1:312
     [Google Scholar]
  7. Davies B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12 . J. Bact. 60:17
     [Google Scholar]
  8. Deacon A. G., Duerden B. I., Holbrook W. P. 1978; Gas-liquid chromatographic analysis of metabolic products in the identification of Bacteroidaceae of clinical interest. J. med. Microbiol. 11:81
     [Google Scholar]
  9. Duerden B. I., Collée J. G., Brown R., Deacon A. G., Holbrook W. P. 1980; A scheme for the identification of clinical isolates of gram-negative anaerobic bacilli by conventional bacteriological tests. J. med. Microbiol. 13:231
     [Google Scholar]
  10. Falkow S. 1975 Infectious multiple drug resistance Pion; London:
     [Google Scholar]
  11. Guerry P., Leblanc D. J., Falkow S. 1973; General method for the isolation of plasmid deoxyribonucleic acid. J. Bact. 116:1064
     [Google Scholar]
  12. Holbrook W. P., Ogston S. A., Ross P. W. 1978; A method for the isolation of Bacteroides melaninogenicus from the human mouth. J. med. Microbiol. 11:203
     [Google Scholar]
  13. Imamura H., Watanabe K., Ezaki T., Sawa K., Mochizuki I., Kobayashi T., Koubata S., Ninomiya K., Ueno K., Suzuki S. 1978; Appearance of clindamycin-resistant Bacteroides fragilis in faeces of patients administered clindamycin. In Current chemotherapy, Proceedings of 10th international congress of chemotherapy I Siegenthaler W., Luthy R. American Society for Microbiology; Washington, DC:304
     [Google Scholar]
  14. Mancini C., Behme R. J. 1977; Transfer of multiple antibiotic resistance from Bacteroides fragilis to Escherichia coli . J. infect. Dis. 136:597
     [Google Scholar]
  15. Møller J. K., Bak A. L., Stenderup A., Zachariae H., Afzelius H. 1977; Changing patterns of plasmid-mediated drug resistance during tetracycline therapy. Antimicrob. Agents Chemother. 11:388
     [Google Scholar]
  16. Nastro L. J., Finegold S. M. 1972; Bactericidal activity of five antimicrobial agents against Bacteroides fragilis . J. infect. Dis. 126:104
     [Google Scholar]
  17. Privitera G., Dublanchet A., Sebald M. 1979a; Transfer of multiple antibiotic resistance between subspecies of Bacteroides fragilis . J. infect. Dis. 139:97
     [Google Scholar]
  18. Privitera G., Sebald M., Fayolle F. 1979b; Common regulatory mechanism of expression and conjugative ability of a tetracycline resistance plasmid in Bacteroides fragilis . Nature, Lond. 278:657
     [Google Scholar]
  19. Rotimi V. O., Duerden B. I. 1980; Curing of antibiotic resistance in Bacteroides species by aminoacridines and ethidium bromide. Afr. J. med. Sci. 8:1
     [Google Scholar]
  20. Rotimi V. O., Duerden B. I., Ede V., Mackinnon A. E. 1979; Metronidazole-resistant Bacteroides from untreated patient. Lancet 1:833
     [Google Scholar]
  21. Rudek W., Haque R. U. 1976; Extracellular enzymes of the genus Bacteroides . J. clin. Microbiol. 4:458
     [Google Scholar]
  22. Salaki J. S., Black R., Tally F. P., Kislak J. W. 1976; Bacteroides fragilis resistant to the administration of clindamycin. Am. J. Med. 60:426
     [Google Scholar]
  23. Saunders J. R. 1978; Anaerobes and transferable drug resistance. Nature, Lond. 274:113
     [Google Scholar]
  24. Stiffler P. W., Keller R., Traub N. 1974; Isolation and characterization of several cryptic plasmids from clinical isolates of Bacteroides fragilis . J. infect. Dis. 130:544
     [Google Scholar]
  25. Tally F. P., Snydman D. R., Gorbach S. L., Malamy M. H. 1979; Plasmid-mediated transferable resistance to clindamycin and erythromycin in Bacteroides fragilis . J. infect. Dis. 139:83
     [Google Scholar]
  26. Watanabe T., Lyang K. W. 1962; Episome-mediated transfer of drug resistance in enterobacteriaceae. V. Spontaneous segregation and recombination of resistance factors in Salmonella typhimurium . J. Bact. 84:422
     [Google Scholar]
  27. Welch R. A., Jones K. R., Macrina F. L. 1979; Transferable lincosamide-macrolide resistance in Bacteroides . Plasmid 2:261
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-14-4-359
Loading
Transferable plasmid-mediated antibiotic resistance in Bacteroides
J. Med. Microbiol. 14, 359 (1981); https://doi.org/10.1099/00222615-14-4-359
/content/journal/jmm/10.1099/00222615-14-4-359
/content/journal/jmm/10.1099/00222615-14-4-359
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error