Responses of peripheral lymphocytes from the blood of 20 healthy volunteers were determined in vitro by measurement of tritiated thymidine uptake in the presence of six different antigens prepared from Candida albicans. Crude antigens (disintegrated cells, culture filtrate) produced greater lymphoblast reactivity than purified preparations (cytoplasmic proteins, cell-wall mannan).
Reactions to soluble mannan were uncommon but the number of subjects who responded to them increased from one to 14 when the material was rendered particulate by adsorption on to latex particles. Lymphocyte responses were also elicited by intact, heat-killed yeast cells.
DuboisM.,
GillesK. A.,
HamiltonJ. K.,
RebersP. A.,
SmithF.1956; Colorimetrie method for determination of sugars and related substances. Analyt. Chem 28:350
LeeK. L.,
CampbellC. C.,
BuckleyH. R.1975; An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans. Sabouraudia 13:148
LloydK. O.1970; The preparation of two insoluble forms of the phytohemagglutinin concanavalin A, and their interactions with polysaccharides and glycoproteins. Archs. Biochem. Biophys 137:460
LongbottomJ. L.,
BrightonW. D.,
EdgeG.,
PepysJ.1976; Antibodies mediating type I skin test reactions to polysaccharide and protein antigens of Candida albicans. Clin. Allerg 6:41
MackenzieD. W. R.,
PhilpotC. M.,
ProctorA. G. J.1980; Basic serodiagnostic methods for diseases caused by fungi and actinomycetes. PHLS monograph series no. 12 H.M.S.O.; London: