1887

Abstract

Summary.

Responses of peripheral lymphocytes from the blood of 20 healthy volunteers were determined by measurement of tritiated thymidine uptake in the presence of six different antigens prepared from . Crude antigens (disintegrated cells, culture filtrate) produced greater lymphoblast reactivity than purified preparations (cytoplasmic proteins, cell-wall mannan).

Reactions to soluble mannan were uncommon but the number of subjects who responded to them increased from one to 14 when the material was rendered particulate by adsorption on to latex particles. Lymphocyte responses were also elicited by intact, heat-killed yeast cells.

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1981-08-01
2022-05-19
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References

  1. Alford R. H. 1973; Transformation of lymphocytes of normal and hospitalized adults by Candida albicans extract. Proc. Soc. exp. Biol. Med 144:826
    [Google Scholar]
  2. Cohen J. J., Rodriguez G. E., Kind P. D., Campbell P. A. 1975; Listeria cell wall fraction: a B cell mitogen. J. Immun 114:1132
    [Google Scholar]
  3. Drake C. H. 1945; Natural antibodies against yeast-like fungi as measured by slide agglutination. J. Immunol 50:185
    [Google Scholar]
  4. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetrie method for determination of sugars and related substances. Analyt. Chem 28:350
    [Google Scholar]
  5. Foroonzanfar N., Yamamura M., Hobbs J. R. 1974; Standardization of lymphocyte transformation to Candida immunogen. Clin. exp. Immun 16:301
    [Google Scholar]
  6. Frisk A., von Stedingk L.-V., Wasserman J. 1974; Lymphocyte stimulation in Candida albicans infections. Sabouraudia 12:87
    [Google Scholar]
  7. Gorin P. A. J., Spencer J. F. T. 1968; Structural chemistry of fungal polysaccharides. Adv. carbohyd. Chem 23:367
    [Google Scholar]
  8. Lee K. L., Campbell C. C., Buckley H. R. 1975; An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans. Sabouraudia 13:148
    [Google Scholar]
  9. Lehrer R. I., Cline M. J. 1969; Interaction of Candida albicans with human leukocytes and serum. J. Bact 98:996
    [Google Scholar]
  10. Lloyd K. O. 1970; The preparation of two insoluble forms of the phytohemagglutinin concanavalin A, and their interactions with polysaccharides and glycoproteins. Archs. Biochem. Biophys 137:460
    [Google Scholar]
  11. Longbottom J. L., Brighton W. D., Edge G., Pepys J. 1976; Antibodies mediating type I skin test reactions to polysaccharide and protein antigens of Candida albicans. Clin. Allerg 6:41
    [Google Scholar]
  12. Mackenzie D. W. R., Philpot C. M., Proctor A. G. J. 1980; Basic serodiagnostic methods for diseases caused by fungi and actinomycetes. PHLS monograph series no. 12 H.M.S.O.; London:
    [Google Scholar]
  13. Möller G. 1969; Induction of DNA synthesis in normal human lymphocyte cultures by antigen-antibody complexes. Clin. exp. Immun 4:65
    [Google Scholar]
  14. Nilsson B. S., Möller G. 1972; Reactivity of human lymphocytes to aggregated and non-aggregated PPD-tuberculin. Cell. Immun 5:555
    [Google Scholar]
  15. Peat S., Whelan W. J., Edwards T. E. 1961; Polysaccharides of baker’s yeast. Part IV. Mannan. J. chem. Soc 1:29
    [Google Scholar]
  16. Pepys J., Faux J. A., Longbottom J. L., McCarthy D. S., Hargreave F. E. 1968; Candida albicans precipitins in respiratory disease in man. J. Allerg 41:305
    [Google Scholar]
  17. Shannon D. C., Johnson G., Rosen F. S., Austen K. F. 1966; Cellular reactivity to Candida albicans antigen. New Engl. J. Med 275:690
    [Google Scholar]
  18. Sultzer B. M., Nilsson B. S. 1972; PPD tuberculin—a B-cell mitogen. Nature new Biol 240:198
    [Google Scholar]
  19. Yu R. J., Bishop C. T., Cooper F. P., Hasenclever H. F., Blank F. 1967; Structural studies of mannans from Candida albicans (serotypes A and B), Candida parapsilosis, Candida stellatoidea and Candida tropicalis. Can. J. Chem 45:2205
    [Google Scholar]
  20. Zabriskie J. B., Falk R. E. 1970; In vitro reactivity of lymphocytes to particulate and soluble antigens. Nature, Lond 226:943
    [Google Scholar]
  21. Zawisza-Zenkteler W., Zamiechowska-Miazga J. 1974; Response of peripheral blood lymphocytes from rabbits to Candida albicans antigens. Bull. Acad. polon. Sci 22:293
    [Google Scholar]
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