H-specific agglutinating sera to Pseudomonas aeruginosa were prepared by immunisation with partially purified flagella. The results of agglutination and immobilisation tests with rabbit sera prepared against the flagella of six H-type strains showed that the sera had high titres and were H specific.
Cross-absorption tests revealed that one strain (H-3) possessed a distinct antigen not present in any of the others. Two groups of strains (H-1, H-2 and H-5; H-4 and H-6) each possessed a distinct major antigen. Members of these two groups could be distinguished by their minor antigens.
AleksićS.,
RohdeR.1972; The separation and purification of Salmonella-Arizona H-antigens by the DEAE-cellulose column chromatography for the preparation of diagnostic H-antisera with high titres and free of O-antibodies. Annls Inst. Pasteur, Paris 123:363
HommaJ. Y.,
KimK. S.,
YamadaH.,
ItoM.,
ShionoyaH.,
KawabeY.1970; Serological typing of Pseudomonas aeruginosa and its cross-infection. Jap. J. exp. Med 40:347
LindbergR. B.,
LattaR.,
BrameR.,
MoncrieffJ. A.1963; Bacteriophage typing of Pseudomonas aeruginosa: epidemiologie observations based on type distribution. Bact. Proc72
NossalG. J. V.,
AdaG. L.,
AustinC. M.1964; Antigens in immunity. II. Immunogenic properties of flagella, polymerized flagellin and flagellin in the primary response. Ausi. J. exp. Biol. med. Sci 42:283
ParkerM. T.,
PittT. L.,
AsheshovE. H.,
MartinD. R.1976; The selection of typing methods for Pseudomonas aeruginosa. In Proceedings of the VI international colloqium on phage typing and other laboratory methods for epidemiological surveillanceWernigerode GDR; 1975 2:469