Preparation of agglutinating antisera specific for the flagellar antigens of Free

Abstract

Summary.

H-specific agglutinating sera to were prepared by immunisation with partially purified flagella. The results of agglutination and immobilisation tests with rabbit sera prepared against the flagella of six H-type strains showed that the sera had high titres and were H specific.

Cross-absorption tests revealed that one strain (H-3) possessed a distinct antigen not present in any of the others. Two groups of strains (H-1, H-2 and H-5; H-4 and H-6) each possessed a distinct major antigen. Members of these two groups could be distinguished by their minor antigens.

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1981-08-01
2024-03-29
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References

  1. Aleksić S., Rohde R. 1972; The separation and purification of Salmonella-Arizona H-antigens by the DEAE-cellulose column chromatography for the preparation of diagnostic H-antisera with high titres and free of O-antibodies. Annls Inst. Pasteur, Paris 123:363
    [Google Scholar]
  2. Ansorg R. 1978; Flagellaspezifisches H Antigenschema von Pseudomonas aeruginosa. Zentbl. Bakt. ParasitKde, I. Abt. Orig 242:228
    [Google Scholar]
  3. Bergan T. 1975; Epidemiological typing of Pseudomonas aeruginosa. In Resistance of Pseudomonas aeruginosa Brown M. R. W. Wiley; New York:189
    [Google Scholar]
  4. Bradley D. E. 1966; The structure and infective process of a Pseudomonas aeruginosa bacteriophage containing ribonucleic acid. J. gen. Microbiol 45:83
    [Google Scholar]
  5. Bradley D. E. 1972; Stimulation of pilus formation in Pseudomonas aeruginosa by RNA bacteriophage adsorption. Biochem. biophys. Res. Commun 47:1080
    [Google Scholar]
  6. Bradley D. E. 1973; Basic characterization of a Pseudomonas aeruginosa pilus-dependent bacteriophage with a long non-contractile tail. J. Virol 12:1139
    [Google Scholar]
  7. Bradley D. E., Pitt T. L. 1974; Pilus-dependence of four Pseudomonas aeruginosa bacteriophages with non-contractile tails. J. gen. Virol 24:1
    [Google Scholar]
  8. Christie R. 1948; Observations on the biochemical and serological characteristics of Pseudomonas pyocyanea. Ausi. J. exp. Biol. med. Sci 26:425
    [Google Scholar]
  9. Ende M. van den. 1952; Observations on the antigenic structure of Pseudomonas aeruginosa. J. Hyg., Camb 50:405
    [Google Scholar]
  10. Habs I. 1957; Untersuchungen über die O-antigene von Pseudomonas aeruginosa. Z. Hyg. InfektKrankh 144:218
    [Google Scholar]
  11. Holloway B. W. 1955; Genetic recombination in Pseudomonas aeruginosa. J. gen. Microbiol 13:572
    [Google Scholar]
  12. Homma J. Y., Kim K. S., Yamada H., Ito M., Shionoya H., Kawabe Y. 1970; Serological typing of Pseudomonas aeruginosa and its cross-infection. Jap. J. exp. Med 40:347
    [Google Scholar]
  13. Lányi B. 1966-67; Serological properties of Pseudomonas aeruginosa I. Group-specific somatic antigens. Acta microbiol. hung 13:295
    [Google Scholar]
  14. Lányi B. 1970; Serological properties of Pseudomonas aeruginosa II. Type-specific thermolabile (flagellar) antigens. Acta microbiol. hung 17:35
    [Google Scholar]
  15. Lindberg R. B., Latta R., Brame R., Moncrieff J. A. 1963; Bacteriophage typing of Pseudomonas aeruginosa: epidemiologie observations based on type distribution. Bact. Proc72
    [Google Scholar]
  16. Liu P. V. 1968; Analytical serology of Pseudomonadaceae. In Analytical serology of microorganisms 2 Kwapinski I. B. G. Wiley; New York:1
    [Google Scholar]
  17. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  18. McDonough M. W. 1965; Amino acid composition of antigenically distinct Salmonella flagellar proteins. J. molec. Biol 12:342
    [Google Scholar]
  19. Moillo A. M. 1973; Isolation of a transducing phage forming plaques on Pseudomonas maltophilia and Pseudomonas aeruginosa. Genet. Res 21:287
    [Google Scholar]
  20. Nossal G. J. V., Ada G. L., Austin C. M. 1964; Antigens in immunity. II. Immunogenic properties of flagella, polymerized flagellin and flagellin in the primary response. Ausi. J. exp. Biol. med. Sci 42:283
    [Google Scholar]
  21. Orcutt M. L. 1924; The effect of heat on flagellar and somatic agglutination. J. exp. Med 40:627
    [Google Scholar]
  22. Parker M. T., Pitt T. L., Asheshov E. H., Martin D. R. 1976; The selection of typing methods for Pseudomonas aeruginosa. In Proceedings of the VI international colloqium on phage typing and other laboratory methods for epidemiological surveillanceWernigerode GDR; 1975 2:469
    [Google Scholar]
  23. Pitt T. L. 1979; A study of the heat-labile antigens of Pseudomonas aeruginosa. Ph.D Thesis Council for National Academic Awards; London:
    [Google Scholar]
  24. Pitt T. L., Bradley D. E. 1975; The antibody response to the flagella of Pseudomonas aeruginosa. J. med. Microbiol 8:97
    [Google Scholar]
  25. Pitt T. L., Erdman Y. J. 1978; The specificity of agglutination reactions of Pseudomonas aeruginosa with O antisera. J. med. Microbiol 11:15
    [Google Scholar]
  26. Sadoff J. C. 1974; Cell-wall structures of Pseudomonas aeruginosa with immunologie significance: a brief review. J. infect. Dis 130:S61
    [Google Scholar]
  27. Trevelyan W. E., Harrison J. S. 1952; Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem. J 50:298
    [Google Scholar]
  28. Verder E., Evans J. 1961; A proposed antigenic schema for the identification of strains of Pseudomonas aeruginosa. J. infect. Dis 109:183
    [Google Scholar]
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