1887

Abstract

SUMMARY.

Three smooth strains of urinary were grown in a chemostat under carbon-limited (C-lim) and magnesium-limited (Mg-lim) conditions over a range of dilution rates (D). Strain LP1674 was resistant to human serum under C-lim but became sensitive when grown under Mg-lim, the degree of sensitivity increasing as D increased. The transition to serum sensitivity was accompanied by loss of ability to produce extractable K1 antigen and a reduction in the amount of a 46k envelope polypeptide. C-lim cells of strain LP729 exhibited a delayed sensitive response to serum, the degree of lag in serum killing becoming less pronounced with increasing values of D; Mg-lim cells were more sensitive with little or no lag in serum killing. The degree of lag appeared to be directly related to the amount of the O side-chain sugar mannose associated with the lipopolysaccharide. C-lim and Mg-lim cultures of strain IP1395 were resistant to serum except when growing at near maximal rates. Although C-lim cultures contained more acidic polysaccharide than Mg-lim cells, transition to serum sensitivity did not appear to be related to exopolysaccharide production. Rapidly growing cells of strain LP1395 did, however, have lower lipopolysaccharide 0 side-chain sugar: core-sugar ratios than more slowly growing cells. With all three strains, changes in dilution rate and in the nature of the limiting nutrient were accompanied by changes in envelope protein composition.

This study demonstrates that many cell-surface changes occur in response to alterations in the growth environment and some of these may be correlated with changes in sensitivity to serum.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-14-1-9
1981-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/14/1/medmicro-14-1-9.html?itemId=/content/journal/jmm/10.1099/00222615-14-1-9&mimeType=html&fmt=ahah

References

  1. Ames G. F. 1974; Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble and periplasmic fractions. J. biol. Chem 249:634
    [Google Scholar]
  2. Barry G. T., Abbott V., Tsai T. 1962; Relationship of colominic acid (poly N-acetyl- neuraminic acid) to bacteria which contain neuraminic acid. J. gen. Microbiol 29:335
    [Google Scholar]
  3. Björkstén B., Bortolussi R., Gothefors L., Quie P.G. 1976; Interaction of E. coli strains with human serum: lack of relationship to K1 antigen. J. Pediat 89:892
    [Google Scholar]
  4. Bortolussi R., Ferrieri P., Wannamaker L.W. 1978; Dynamics of Escherichia coli infection and meningitis in infant rats. Infect. Immun 22:480
    [Google Scholar]
  5. Dische Z. 1947; A new specific color reaction of hexuronic acids. J. biol. Chem 167:189
    [Google Scholar]
  6. Evans C. G. T., Herbert D., Tempest D. W. 1970; The continuous cultivation of micro-organisms. 2. Construction of a chemostat. In Methods in microbiology 2 Norris J. R., Ribbons D. W. Academic Press; London:277
    [Google Scholar]
  7. Feingold D. S. 1969; The serum bactericidal reaction, IV. Phenotypic conversion of Escherichia coli from serum-resistance to serum-sensitivity by diphenylamine. J. infect. Dis 120:437
    [Google Scholar]
  8. Glynn A. A., Howard C.J. 1970; The sensitivity to complement of strains of Escherichia coli related to their K antigens. Immunology 18:331
    [Google Scholar]
  9. Holme T., Lindberg A. A., Garegg P. J., Onn T. 1968; Chemical composition of cell wall polysaccharide of rough mutants of Salmonella typhimurium . J. gen. Microbiol 52:45
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, Lond 227:680
    [Google Scholar]
  11. McElree H., Pitcher J., Arnwine J. 1966; Transiently acquired serum resistance by cell-grown Escherichia coli . J. infect. Dis 116:231
    [Google Scholar]
  12. Olling S. 1977; Sensitivity of gram-negative bacilli to the serum bactericidal activity: a marker of the host–parasite relationship in acute and persisting infections. Scand. J. infect. Dis suppl 10:1
    [Google Scholar]
  13. Parton R. 1975; Envelope proteins in Salmonella minnesota mutants. J. gen. Microbiol 89:113
    [Google Scholar]
  14. Reske K., Jann K. 1972; The O8 antigen ofEscherichia coli Structure of the polysaccharide chain. Eur. J. Biochem 31:320
    [Google Scholar]
  15. Rittenberg S. C., Penn C. W., Parsons N. J., Veale D. R., Smith H. 1977; Phenotype changes in the resistance of Neisseria gonorrhoeae to killing by normal human serum. J. gen. Microbiol 103:69
    [Google Scholar]
  16. Robbins J.B., McCracken G. H. Jr., Gotschlich E. C., Ørskov F., Ørskov I., Hanson L.A. 1974; Escherichia coli K1 capsular polysaccharide associated with neonatal meningitis. N. Engl. J. Med 290:1216
    [Google Scholar]
  17. Sarff L.D., McCracken G. H. Jr., Schiffer M. S., Glode M. P., Robbins J. B., Ørskov I., Ørskov F. 1975; Epidemiology of Escherichia coli K1 in healthy and diseased newborns. Lancet 1:1099
    [Google Scholar]
  18. Schlecht S., Westphal O. 1967; Uber die Herstellung von Antiseren gegen die soma- tischen (O-) Antigene von Salmonellen. I. Mitteilung: Untersuchungen uber Agglutinin- titer. Zentbl. Bakt. ParasitKde I. Abt. Orig 204:335
    [Google Scholar]
  19. Schmidt G., Fromme I., Mayer H. 1970; Immunochemical studies on core lipopolysac- charides of Enterobacteriaceae of different genera. Eur. J. Biochem 14:357
    [Google Scholar]
  20. Smith H. 1977; Microbial surfaces in relation to pathogenicity. Bact. Rev 41:475
    [Google Scholar]
  21. Taylor P. W. 1975; Genetical studies of serum resistance in Escherichia coli . J. gen. Microbiol 89:57
    [Google Scholar]
  22. Taylor P. W. 1976; Immunochemical investigations on lipopolysaccharides and acidic polysaccharides from serum-sensitive and serum-resistant strains of Escherichia coli isolated from urinary-tract infections. J. med. Microbiol 9:405
    [Google Scholar]
  23. Taylor P. W. 1978; The effect of the growth environment on the serum sensitivity of some urinary Escherichia coli strains. F.E.M.S. microbiol. Lett 3:119
    [Google Scholar]
  24. Taylor P. W., Hughes C. 1978; Plasmid carriage and the serum sensitivity of enterobac- teria. Infect. Immun 22:10
    [Google Scholar]
  25. Taylor P. W., Hughes C., Robinson M. 1979; Plasmids and the serum resistance of enterobacteria. In Plasmids of medical, environmental and commercial importance Timmis K. N., Pühler A. Elsevier/North Holland Biomedicai Press; Amsterdam:135
    [Google Scholar]
  26. Taylor P. W., Parton R. 1977; A protein factor associated with serum resistance in Escherichia coli . J. med. Microbiol 10:225
    [Google Scholar]
  27. Taylor P. W., Sleytr U. B. 1977; Release of a lysogenic bacteriophage from a smooth urinary E. coli strain following magnesium limitation in the chemostat. F.E.M.S. microbiol. Lett 2:189
    [Google Scholar]
  28. Wardlaw A. C. 1963; The complement-dependent bacteriolytic activity of normal human serum. II. Cell wall composition of sensitive and resistant strains. Can. J. Microbiol 9:41
    [Google Scholar]
  29. Warren L. 1959; The thiobarbituric acid assay of sialic acids. J. biol. Chem 234:1971
    [Google Scholar]
  30. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. In Methods in carbohydrate chemistry 5 Whistler R. L. Academic Press; London:83
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-14-1-9
Loading
/content/journal/jmm/10.1099/00222615-14-1-9
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error