1887

Abstract

Summary.

The production of neuraminidase (EC 3.2.1.18) by 77 strains of Bacteroidaceae was investigated by techniques previously used to study neuraminidase production by clostridia. Conditions for culture and assay of neuraminidase were characterised. The enzyme is predominantly cell associated; it is not calcium dependent and the H optimum for its production is . 4.5.

Most neuraminidase-positive strains produced the enzyme well in the test media but a few strains failed to produce it consistently in one or other of the media. Because of these occasional variations, strains were grown and tested in at least two media before being defined as neuraminidase negative.

Within the group of species, , , , , and were neuraminidase positive while , and were negative. Two subspecies of (ss. and ss. ) were positive but the other (ss. ) was negative. Strains of and produced the enzyme while , , and did not. The microaerophilic were also positive. None of the or species tested produced neuraminidase.

Our results for neuraminidase production are consistent for all strains of each species examined and we suggest that tests for neuraminidase production would be a valuable addition to biochemical tests currently used in taxonomic studies of the Bacteroidaceae.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-14-1-63
1981-02-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/14/1/medmicro-14-1-63.html?itemId=/content/journal/jmm/10.1099/00222615-14-1-63&mimeType=html&fmt=ahah

References

  1. Brunetti P., Swanson A., Roseman S. 1963; Enzymatic determination of sialic acids. N-Acylneuraminic acid ⇆ N-Acyl-D-mannosamine + pyruvate. In Methods in enzymology Colowick S. P., Caplan N. O. Academic Press; New York and London: 6465
    [Google Scholar]
  2. Collee J. G. 1965; The relationship of the haemagglutinin of Clostridium welchii to the neuraminidase and other soluble products of the organism. J. Path. Bact 90:13
    [Google Scholar]
  3. Cruickshank R., Duguid J. P., Marmion B. P., Swain R. H. A. 1975 Medical microbiology, 12th. Churchill Livingstone; Edinburgh: 2111
    [Google Scholar]
  4. Deacon A. G., Duerden B. I., Holbrook W. P. 1978; Gas-liquid chromatographic analysis of metabolic products in the identification of Bacteroidaceae of clinical interest. J. med. Microbiol 11:81
    [Google Scholar]
  5. Drzeniek R. 1972; Viral and bacterial neuraminidases. Curr. Top. Microbiol. Immun 59:35
    [Google Scholar]
  6. Duerden B. I. 1980a; The isolation and identification of Bacteroides spp. from the normal human faecal flora. J. med. Microbiol 13:69
    [Google Scholar]
  7. Duerden B. I. 1980b; The isolation and identification of Bacteroides spp. from normal human vaginal flora. J. med. Microbiol 13:79
    [Google Scholar]
  8. Duerden B. I., Collee J. G., Brown R., Deacon A. G., Holbrook W. P. 1980; A scheme for the identification of clinical isolates of gram-negative anaerobic bacilli by conventional bacteriological tests. J. med. Microbiol 13:231
    [Google Scholar]
  9. Duerden B. I., Holbrook W. P., Collee J. G., Watt B. 1976; The characterization of clinically important gram-negative anaerobic bacilli by conventional bacteriological tests. J. appl. Bact 40:163
    [Google Scholar]
  10. Finegold S. M. 1977 Anaerobic bacteria in human disease Academic Press; New York:
    [Google Scholar]
  11. Fraser A. G. 1978; Neuraminidase production by clostridia. J. med. Microbiol 11:269
    [Google Scholar]
  12. Fraser A. G., Collee J. G. 1975; The production of neuraminidase by food-poisoning strains of Clostridium welchii (C. perfringens). J. med. Microbiol 8:251
    [Google Scholar]
  13. Fraser A. G., Smith J. K. 1975; Preparation of a glycoprotein fraction from pooled human plasma and its evaluation as a substrate for the assay of Clostridium welchii (C. perfringens) neuraminidase. J. med. Microbioh 8:235
    [Google Scholar]
  14. Fukui K., Fukui Y., Moriyama T. 1971; Neuraminidase activity in some bacteria from the human mouth. Archs oral Biol 16:1361
    [Google Scholar]
  15. Holbrook W. P., Duerden B. I. 1974; A comparison of some characteristics of reference strains of Bacteroides oralis with Bacteroides melaninogenicus . Archs oral Biol 19:1231
    [Google Scholar]
  16. Holbrook W. P., Duerden B. I., Deacon A. G. 1977; The classification of Bacteroides melaninogenicus and related species. J. appl. Bact 42:259
    [Google Scholar]
  17. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe laboratory manual, 4th. Virginia Polytechnic Institute and State University; Blacksburg, Virginia:
    [Google Scholar]
  18. Holdeman L. V., Moore W. E. C. 1974; Bacteroidaceae. In Bergey’s manual of determinative bacteriology, 8th. Buchanan R. E., Gibbons N. E. Williams & Wilkins; Baltimore:384
    [Google Scholar]
  19. Müller H. E. 1973; The occurrence of neuraminidase and acylneuraminate pyruvate-lyase in Fusobacterium polymorphum and their pathogenetic role. Zentbl. Bakt. ParasitKde, I. Abt. Orig 224:212 in German
    [Google Scholar]
  20. Müller H. E. 1974; Neuraminidases of bacteria and protozoa and their pathogenetic role. Behring Inst. Mitt5534
    [Google Scholar]
  21. Müller H. E., Werner H. 1970a; Neuraminidase as a pathogenic factor in an abscess caused by Bacteroides fragilis . Z. med. Mikrobiol. Immun 156:98 in German
    [Google Scholar]
  22. Müller H. E., Werner H. 1970b; In vitro studies in the occurrence of neuraminidase in Bacteroides species. Pathologia Microbioh 36:135 in German
    [Google Scholar]
  23. Nees S., Schauer R. 1974; Induction of neuraminidase from Clostridium perfringens and the cooperation of this enzyme with acylneuraminate pyruvate-lyase. Behring Inst. Mitt5568
    [Google Scholar]
  24. Nees S., Schauer R., Mayer F., Ehrlich K. 1976; Purification and characterisation of N-acetylneuraminate lyase from Clostridium perfringens . Hoppe-Seyler’s Z. physiol. Chem 357:839
    [Google Scholar]
  25. Newman M. G., Sutter V. L., Pickett M. J., Blachman U., Greenwood J. R., Grinenko V., Citron D. 1979; Detection, identification, and comparison of Capnocytophaga, Bacteroides ochraceus, and DF-1. J. clin. Microbioh 10:557
    [Google Scholar]
  26. Rosenberg A., Schengrund C.-L. 1976; Sialidases. In Biological roles of sialic acid Rosenberg A., Schengrund C.-L. Plenum Press; New York:295
    [Google Scholar]
  27. Solovev V. D., Domaradsky I. V., Shimanyuk N. Ya., Bichul K. G., Kurennaya I. I. 1972; Function of neuraminidase in Vibrio taxonomy. Bull. exp. Biol. Med. U. S. S. R 73:61
    [Google Scholar]
  28. Werner H. 1974; Differentiation and medical importance of saccharolytic intestinal Bacteroides. Arzneimittel-Forsch 24:340
    [Google Scholar]
  29. Werner H., Müller H. E. 1971; Immunelectrophoretic studies on the action of Bacteroides, Fusobacterium, Leptotrichia, Sphaerophorus species on human plasma proteins. Zentbl. Bakt. ParasitKde, I. Abt. Orig 216:96 in German
    [Google Scholar]
  30. Williams B. L., Hollis D., Holdeman L. V. 1979; Synonymy of strains of Center for Disease Control group DF-1 with species of Capnocytophaga. J. clin. Microbioh 10:550
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-14-1-63
Loading
/content/journal/jmm/10.1099/00222615-14-1-63
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error