The pathogenicity of and related species estimated by intracutaneous infection in the guinea-pig Free

Abstract

SUMMARY.

The pathogenicity of 42 strains of of human origin was estimated by intracutaneous injection of bacterial suspensions into guinea-pig skin. Comparisons of living and heat-killed suspensions revealed that . strains maintained themselves and possibly multiplied in the skin, whereas the lesions induced by non-fra-gilis strains appeared to be due mainly to toxicity. Measurement of skin pathogenicity in terms of the number of viable organisms in the inoculum that produced a lesion 10 mm in diameter showed that . was, on average, 17 times as pathogenic as non-fragilis strains.

Skin tests of pathogens may be of value in the analysis of virulence factors of and possibly of other anaerobic organisms.

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1981-02-01
2024-03-29
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References

  1. Altemeier W. A. 1942; The pathogenicity of the bacteria of appendicitis peritonitis: experimental study. Surgery 11:374
    [Google Scholar]
  2. Babb J. L., Cummins C. S. 1978; Encapsulation of Bacteroides species. Infect. Immun 19:1088
    [Google Scholar]
  3. Casciato D. A., Rosenblatt J. E., Bluestone R., Goldberg L. S., Finegold S. M. 1979; Susceptibility of isolates of Bacteroides to the bactericidal activity of normal human serum. J. infect. Dis 140:109
    [Google Scholar]
  4. Cato E. P., Johnson J. L. 1976; Reinstatement of species rank for Bacteroides fragilis, Bovatus, B. distasonis, B. thetaiotaomicron and B. vulgatus: designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides thetaiotaomicron (Distaso) Castellani and Chalmers. Int. J. syst. Bad 26:230
    [Google Scholar]
  5. Duerden B. I., Holbrook W. P., Collee J. G., Watt B. 1976; The characterization of clinically important gram-negative anaerobic bacilli by conventional bacteriological tests. J.appl.Bact 40:163
    [Google Scholar]
  6. Finegold S. M., Attebery H. R., Sutter V. L. 1974; Effect of diet on human fecal flora: comparison of Japanese and American diets. Am. J. clin. Nutr 27:1456
    [Google Scholar]
  7. Hill G. B., Osterhout S., Pratt P. C. 1974; Liver abscess production by non-spore-forming anaerobic bacteria in a mouse model. Infect. Immun 9:599
    [Google Scholar]
  8. Hite K. E., Locke M., Hesseltine H. C. 1949; Synergism in experimental infections with nonsporulating anaerobic bacteria. J. infect. Dis 84:1
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe laboratory manual. , 4. Virginia Polytechnic Institute and State University; Blacksburg, Virginia:
    [Google Scholar]
  10. Ingham H. R., Sisson P. R., Selkon J. B. 1980; Current concepts of the pathogenetic mechanisms of non-sporing anaerobes: chemotherapeutic implications. J. antimicrob. Chemother 6:173
    [Google Scholar]
  11. Ingham H. R., Sisson P. R., Tharagonnet D., Selkon J. B., Codd A. A. 1977; Inhibition of phagocytosis in vitro by obligate anaerobes. Lancet 2:1252
    [Google Scholar]
  12. Jones R. N., Fuchs P. C. 1976; Identification and antimicrobial susceptibility of 250 Bacteroides fragilis subspecies tested by broth microdilution methods. Antimicrob. Agents Chemother 9:719
    [Google Scholar]
  13. Kelly M. J. 1978; The quantitative and histological demonstration of pathogenic synergy between Escherichia coli and Bacteroides fragilis in guinea-pig wounds. J. med. Microbiol 11:513
    [Google Scholar]
  14. Leigh D. A. 1974; Clinical importance of infections due to Bacteroides fragilis and role of antibiotic therapy. Br. med. J 3:225
    [Google Scholar]
  15. Miles A. A., Miles E. M. 1952; Vascular reactions to histamine, histamine-liberator and leukotaxine in the skin of guinea-pigs. J. Physiol 118:228
    [Google Scholar]
  16. Miles A. A., Miles E. M., Burke J. 1957; The value and duration of defence reactions of the skin to the primary lodgement of bacteria. Br. J. exp. Path 38:79
    [Google Scholar]
  17. Miles A. A., Misra S. A., Irwin J. O. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb 38:732
    [Google Scholar]
  18. Miles A. A., Pillow J., Khimji P. L. 1976; The action of iron on local klebsiella infection of the skin of the guinea-pig and its relation to the decisive period in primary infective lesions. Br. J. exp. Path 57:217
    [Google Scholar]
  19. Onderdonk A.B., Kasper D. L., Cisneros R. L., Bartlett J. G. 1977; The capsular polysaccharide of Bacteroides fragilis as a virulence factor: comparison of the pathogenic potential of encapsulated and unencapsulated strains. J. infect. Dis 136:82
    [Google Scholar]
  20. Rudek W., Haque R. U. 1976; Extracellular enzymes of the genus Bacteroides. J. clin. Microbiol 4:458
    [Google Scholar]
  21. Suiter V. L., Finegold S. M. 1971; Antibiotic disc susceptibility tests for rapid presumptive identification of Gram-negative anaerobic bacilli. Appl. Microbiol 21:13
    [Google Scholar]
  22. Weinstein W. M., Onderdonk A. B., Bartlett J. G., Gorbach S. L. 1974; Experimental intra-abdominal abscesses in rats: development of an experimental model. Infect. Immun 10:1250
    [Google Scholar]
  23. Wilkins T. D., Walker C. B., Nitzan D., Salyers A. A. 1977; Experimental infections with anaerobic bacteria in mice. J. infect. Dis 135: suppl S 13
    [Google Scholar]
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