%0 Journal Article %A HEARN, VERONICA M. %A MACKENZIE, D. W. R. %T Analysis of wall antigens of Aspergillus fumigatus by two-dimensional immunoelectrophoresis Plates VI–XII %D 1981 %J Journal of Medical Microbiology, %V 14 %N 1 %P 119-129 %@ 1473-5644 %R https://doi.org/10.1099/00222615-14-1-119 %I Microbiology Society, %X Summary. Water-soluble and surface-located antigens from Aspergillus fumigatus mycelium were analysed by two-dimensional electrophoresis (2D IEP) with selected sera. A complex pattern was seen when a water-soluble fraction reacted with hyperimmune sera. Significantly fewer precipitin peaks were seen when wall-located antigens were used in the 2D IEP system; this made possible the detection of a small number of recurring antigen-antibody interactions when sera from patients with aspergilloma or allergic bronchopulmonary aspergillosis were analysed. Many of these recurring precipitates were affected by lectins with specificity for α-D-glucose, α-D-mannose or N-acetyl-D-glucosamine. All the antigens were susceptible to hydrolysis by pronase, confirming the glycoprotein composition of many of these macromolecules. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-14-1-119