1887

Abstract

Summary.

Water-soluble and surface-located antigens from mycelium were analysed by two-dimensional electrophoresis (2D IEP) with selected sera. A complex pattern was seen when a water-soluble fraction reacted with hyperimmune sera. Significantly fewer precipitin peaks were seen when wall-located antigens were used in the 2D IEP system; this made possible the detection of a small number of recurring antigen-antibody interactions when sera from patients with aspergilloma or allergic bronchopulmonary aspergillosis were analysed. Many of these recurring precipitates were affected by lectins with specificity for -D-glucose, -D-mannose or N-acetyl-D-glucosamine. All the antigens were susceptible to hydrolysis by pronase, confirming the glycoprotein composition of many of these macromolecules.

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1981-02-01
2022-05-20
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References

  1. Axelsen N. H., Bock E., Krøll J. 1973; Comparison of antisera. Scand. J. Immunol 2:1101
    [Google Scholar]
  2. Azuma I., Kimura H., Hirao F., Tsubura E., Yamamura Y. 1967; Skin-testing and precipitation antigens from Aspergillus fumigatus for diagnosis of aspergillosis. Am. Rev. resp. Dis 95:305
    [Google Scholar]
  3. Azuma I., Kimura H., Yamamura Y. 1968; Purification and characterization of an immunologically active glycoprotein from Aspergillus fumigatus . J. Bact 96:272
    [Google Scholar]
  4. Bardana E. J., McClatchy J. K., Farr R. S., Minden R. 1972; The primary interaction of antibody to components of aspergilli. I. Immunologic and chemical characteristics of a non precipitating antigen. J. Allergy clin. Immunol 50:208
    [Google Scholar]
  5. Biguet J., Tran Van Ky P., Fruit J., Andrieu S. 1967; Identification d’une activite chymotrypsique au niveau de fractions remarquables de l’extrait antigènique d’ Aspergillus fumigatus Repercussions sur le diagnostic immunologique de l’aspergillose. Rev. Immunol., Paris 31:317
    [Google Scholar]
  6. Bøg-Hansen T. C., Brogren C.-H., McMurrough J. 1974; Identification of enzymes as glycoproteins containing glucose or mannose. Interaction of barley malt proteins with concanavalin A in electrophoresis. J. Inst. Brew 80:443
    [Google Scholar]
  7. BøG-Hansen T. C., Bjerrum O. J., Ramlau J. 1975; Detection of biospecific interaction during the first dimension electrophoresis in crossed immunoelectrophoresis. Scand. J. Immunol 4:2141
    [Google Scholar]
  8. Domer J. E. 1976; In vivo and in vitro cellular responses to cytoplasmic and cell wall antigens of Histoplasma capsulatum in artificially immunized or infected guinea pigs. Infect. Immun 13:790
    [Google Scholar]
  9. Dubois M, Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analyt. Chem 28:350
    [Google Scholar]
  10. Frasch C. E. 1976; In Dialog. 13 Removal of detergent by ultrafiltration Amicon B.V.; Oosterhaut:
    [Google Scholar]
  11. Hearn V. M., Mackenzie D. W. R. 1979; The preparation and chemical composition of fractions from Aspergillus fumigatus wall and protoplasts possessing antigenic activity. J. gen. Microbiol 11235
    [Google Scholar]
  12. Hearn V. M., Mackenzie D. W. R. 1980; Antigenic activity of sub-cellular fractions of Aspergillus fumigatus . Zentbl. Bakt. ParasitKde8173
    [Google Scholar]
  13. Hearn V. M., Proctor A. G., Mackenzie D. W. R. 1980a; The preparation and partial characterization of antigenic fractions obtained from the mycelial walls of several Aspergillus species. J. gen. Microbiol 119:41
    [Google Scholar]
  14. Hearn V. M., Wilson E. V., Proctor A. G., Mackenzie D. W. R. 1980b; Preparation of Aspergillus fumigatus antigens and their analysis by two-dimensional immunoelectrophoresis. J. med. Microbiol 13:451
    [Google Scholar]
  15. Holloway P. W. 1973; A simple procedure for removal of Triton X-100 from protein samples. Analyt. Biochem 53:304
    [Google Scholar]
  16. Huppert M., Spratt N. S., Vukovich K. R., Sun S. H., Rice E. H. 1978; Antigenic analysis of coccidiodin and spherulin determined by two-dimensional immunoelectrophoresis. Infect. Immun 20:541
    [Google Scholar]
  17. Kim S. J., Chaparas S. D., Brown T. M., Anderson M. C. 1978; Characterization of antigens from Aspergillus fumigatus II. Fractionation and electrophoretic, immunologic and biologic activity. Am. Rev. resp. Dis 118:553
    [Google Scholar]
  18. Longbottom J. L. 1978; Immunological aspects of infection and allergy due to Aspergillus species. Mykosen1207
    [Google Scholar]
  19. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  20. Pine L., Boone C. J., McLaughlin D. 1966; Antigenic properties of the cell wall and other fractions of the yeast form of Histoplasma capsulatum . J. Bact 91:2158
    [Google Scholar]
  21. Reiss E., Mitchell W. O., Stone S. H., Hasenclever H. F. 1974a; Cellular immune activity of galactomannan-protein complex from mycelia of Histoplasma capsulatum . Infect. Immun 10:802
    [Google Scholar]
  22. Reiss E., Stone S. H., Hasenclever H. F. 1974b; Serological and cellular immune activity of peptidoglucomannan fractions of Candida albicans cell walls. Infect. Immun 9:881
    [Google Scholar]
  23. Salvin S. B., Ribi E. 1955; Antigens from yeast phase of Histoplasma capsulatum II. Immunologic properties of protoplasm vs. cell walls. Proc. soc. expt. Biol. Med 90:287
    [Google Scholar]
  24. Steinbuch M., Audran R. 1969; The isolation of IgG from mammalian sera with the aid of caprylic acid. Archs Biochem. Biophys 134:279
    [Google Scholar]
  25. Stoddart R. W., Herbertson B. M. 1978; The use of fluorescein-labelled lectins in the detection and identification of fungi pathogenic for man: a preliminary study. J. med Microbiol 11:315
    [Google Scholar]
  26. Suzuki S., Suzuki M., Yokota K., Sunayama H., Sakaguchi O. 1967; On the immunochemical and biochemical studies of fungi. XI. Cross-reaction of the polysaccharides of Aspergillus fumigatus, Candida albicans, Saccharomyces cerevisiae and Trichophyton rubrum against Candida albicans and Saccharomyces cerevisiae antisera. Jap. J. Microbiol 11:269
    [Google Scholar]
  27. Tran Van Ky P., Biguet J., Fruit J. 1966; Localisation et fréquence des arcs des immunoélectrophorégrammes produits par le sérum des malades atteints de mycétomes aspergillaires appliqués contre l’antigène Aspergillus fumigatus . Rev. Immunol, Paris 30:13
    [Google Scholar]
  28. Wada I. 1960; Immunological studies on aspergillosis. I. Immunological reactions in experimental aspergillosis by the filtrate from ground mycelia of Aspergillus fumigatus . Jap. J. Bact 15:528
    [Google Scholar]
  29. Yokota K., Shimada H., Kamaguchi A., Sakaguchi O. 1977; Studies on the toxin of Aspergillus fumigatus VII. Purification and some properties of hemolytic toxin (asp-hemo-lysin) from culture filtrates and mycelia. Microbiol. Immunol 21:11
    [Google Scholar]
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