1887

Abstract

SUMMARY.

Virus-like particles similar in size and morphology to the Otofuke agent, which was detected first in an institutional outbreak of acute gastroenteritis at Otofuke, Hokkaido, Japan in 1978, were again observed in faeces from patients in four other outbreaks of acute gastroenteritis in Hokkaido.

In each of three outbreaks in which the faeces and paired sera of patients were available, apparent sero-responses against the particles obtained in corresponding outbreaks were confirmed. Serological analysis of the particles from five separate epidemics by immune elec-tronmicroscopy suggested that the particles from these epidemics may be related antigenically to one another.

Besides the above-mentioned particles measuring 35–40 nm in diameter, 15–20-nm “empty” particles were also found in the stool specimens obtained from two of the five epidemics. In addition, serological cross reactivity between these two kinds of particles was observed.

These findings provide further support for the aetiological role of the 35–40-nm particles (Otofuke agent-like particles) in acute gastroenteritis in older children and adults.

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1981-02-01
2024-04-25
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References

  1. Appleton H., Buckley M., Thom B. T., Cotton J. L., Henderson S. 1977; Virus-like particles in winter vomiting disease. Lancet 1:409
    [Google Scholar]
  2. Appleton H., Pereira M. S. 1977; A possible virus aetiology in outbreaks of food-poisoning from cockles. Lancet 1:780
    [Google Scholar]
  3. Ashley C. R., Caul E. O., Paver W. K. 1978; Astrovirus-associated gastroenteritis in children. J. din. Path 31:939
    [Google Scholar]
  4. Clarke S. K. R., Cook G. T., Egglestone S. I., Hall T. S., Miller D. L., Reed S. E., Rubenstein D., Smith A. J., Tyrrell D. A. J. 1972; A virus from epidemic vomiting disease. Br. med. J 3:86
    [Google Scholar]
  5. Dolin R. 1978; Norwalk agent-like particles associated with gastroenteritis in human beings. J. Am. vet. med. Ass 173:615
    [Google Scholar]
  6. Holmes I. H. 1979; Viral gastroenteritis. Prog. med. Virol 25:1
    [Google Scholar]
  7. McSwiggan D. A., Cubitt D., Moore W. 1978; Calicivirus associated with winter vomiting disease. Lancet 1:1215
    [Google Scholar]
  8. Madeley C. R. 1979; Viruses in the stools. J. din. Path 32:1
    [Google Scholar]
  9. Madeley C. R., Cosgrove B. P. 1975; Viruses in infantile gastroenteritis. Lancet 2:124
    [Google Scholar]
  10. Madeley C. R., Cosgrove B. P. 1976; Caliciviruses in man. Lancet 1:199
    [Google Scholar]
  11. Takahashi E., Konishi S., Ogata M. 1971; Studies on cytopathogenic viruses from cats with respiratory infections. II. Characterization of feline picornaviruses. Jap. J. vet. Sci 33:81
    [Google Scholar]
  12. Taniguchi K., Urasawa S., Urasawa T. 1979; Virus-like particle, 35 to 40 nm, associated with an institutional outbreak of acute gastroenteritis in adults. J. clin. Microbiol 10:730
    [Google Scholar]
  13. Thornhill T. S., Wyatt R. G., Kalica A. R., Dolin R., Chanock R. M., Kapikian A. Z. 1977; Detection by immune electron microscopy of 26- to 27-nm viruslike particles associated with two family outbreaks of gastroenteritis. J. infect. Dis 135:20
    [Google Scholar]
  14. Urasawa T., Urasawa S., Taniguchi K. 1979; Reactivity of neutralizing antibodies with different specificities to H particles of poliovirus. Microbiol. Immunol 23:651
    [Google Scholar]
  15. Woode G. N., Bridger J. C. 1978; Isolation of small viruses resembling astroviruses and caliciviruses from acute enteritis of calves. J. med. Microbiol 11:441
    [Google Scholar]
  16. Youngner J. S. 1954; Monolayer tissue cultures. I. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells. Proc. Soc. exp. Biol. Med 85:202
    [Google Scholar]
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