1887

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Volume 13, Issue 4

IMPROVED MEDIA FOR THE CULTURE OF Free

Abstract

SUMMARY

The ANM medium of Hafiz and McEntegart (1976) was found to be deficient in ability to support the growth of various strains of . Strains that did grow required a large starting inoculum which invariably suffered a substantial drop in the number of viable organisms during the first 2 h. Investigation of the various deficiencies led to the development of liquid (G77L) and solid (G77S) media which were shown to have several advantages over existing media for the cultivation of . These media, which are simple to prepare, do not contain blood or serum and yet gave excellent growth. The inclusion of antibiotics, glucose and phenol red allowed the selective isolation and partial identification of from routine specimens. These media also gave good results in fermentation tests for the identification of neisseriae.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© 1980 The Pathological Society of Great Britain and Ireland
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1980-11-01
2024-04-24
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References

  1. Catlin B. W. 1973; Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J. infect. Dis 128:178
     [Google Scholar]
  2. Chan K., Wiseman G. M., Caird J. D. 1975; Cultivation of type 1 Neisseria gonorrhoeae in liquid media. Br. J. ten. Dis 51:382
     [Google Scholar]
  3. Gerhardt P., Heden C. G. 1960; Concentrated culture of gonococci in clear liquid medium. Proc. Soc. exp. Biol. Med 105:49
     [Google Scholar]
  4. Hafiz S., McEntegart M. G. 1976; Prolonged survival of Neisseria gonorrhoeae in a new liquid medium. Br. J. ven. Dis 52:381
     [Google Scholar]
  5. Kellogg D. S., Cohen I. R., Norins L. C., Schroeter A. L., Reising G. 1968; Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro. J. Bad 96:596
     [Google Scholar]
  6. Kellogg D. S., Peacock W. L., Deacon W. E., Brown L., Pirkle C. I. 1963; Neisseria gonorrhoeae. I. Virulence genetically linked to clonal variation. J. Bad 85:1274
     [Google Scholar]
  7. Kenny C. P., Ashton F. E., Diena B. B., Greenberg L. 1967; A chemically defined protein-free liquid medium for the cultivation of some species of Neisseria. Bull. Wld Hlth Org 37:569
     [Google Scholar]
  8. Lankford C. E. 1950; Chemically defined nutrient supplements for gonococcus culture media. Bad. Proc. 50:40
     [Google Scholar]
  9. Lankford C. E., Skaggs P. K. 1946; Cocarboxylase as a growth factor for certain strains of Neisseria gonorrhoeae. Arch. Biochem 9:265
     [Google Scholar]
  10. Lankford C. E., Snell E. E. 1943; Glutamine as a growth factor for certain strains of Neisseria gonorrhoeae. J. Bad 45:410
     [Google Scholar]
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IMPROVED MEDIA FOR THE CULTURE OF NEISSERIA GONORRHOEAE
J. Med. Microbiol. 13, 597 (1980); https://doi.org/10.1099/00222615-13-4-597
/content/journal/jmm/10.1099/00222615-13-4-597
/content/journal/jmm/10.1099/00222615-13-4-597
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