1887

Abstract

There are difficulties in the diagnosis of deep-seated candidosis; the clinical features are non-specific and similar to bacterial infections, and blood cultures may be negative even when there is an active invasion of the deep tissues.

Serology, especially precipitin tests, are considered a useful diagnostic adjunct (Taschdjian, Seelig and Kozinn, 1973; Odds, 1979) but precipitins to extracts of have been detected in sera from apparently normal subjects (Douchet and Müller, 1972; Everall, Morris and Morris, 1974), pregnant women (Stanley, Hurley and Carroll, 1972), burned patients (Oblack, Schwartz and Holder, 1976), patients given intravenous hyperalimentation (Glew , 1975) and in patients after open heart surgery (Murray, Buckley and Turner, 1969; Parsons and Nassau, 1974; Evans and Forster, 1976). These 'false positive' antibodies may often be directed to the cell-wall mannans of (Pepys , 1968).

Although attempts have been made to introduce new tests for candida antibodies (Stickle , 1972; Müller, 1974) it is unlikely that any serological test can be more specific than the antigenic reagents on which it is based. Most efforts to overcome the problem of poor specificity have involved either the modification of candida extracts by removal of the mannan antigens (Long-bottom , 1976; Syverson and Buckley, 1977; Syverson, Buckley and Gibian, 1978) or a search for novel antigenic components unique to the hyphal form of the fungus (Evans , 1973; Syverson, Buckley and Campbell, 1975). Staib and his co-workers (1977 and ) have suggested that an inducible acid proteinase secreted by may be a useful diagnostic antigen; but they appear to have tested only unrefined culture filtrates containing the enzyme, with equivocal results.

This paper describes a simple method for the rapid purification of proteinase which is a development of the procedure of Remold, Fasold and Staib (1968). The value of the purified enzyme as an antigen for use in the diagnosis of systemic candidosis is assessed in tests with sera from experimental animals and humans.

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1980-08-01
2024-03-29
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References

  1. Axelsen N. H., Krøll J., Weeke B. 1973; A manual of quantitative immunoelectrophoresis, methods and applications. Scand. J. Immunol suppl. 1
    [Google Scholar]
  2. Barrett A. J. 1977; In Proteinases in mammalian cells and tissues. edited by Barrett A. J. Elsevier; Amsterdam: p 1
    [Google Scholar]
  3. Budtz-Jørgensen E. 1974; Proteolytic activity of Candida spp. as related to the pathogenesis of denture stomatitis. Sabouraudia 12:266
    [Google Scholar]
  4. Cutler J. E., Friedman L., Milner K. C. 1972; Biological and chemical characterization of toxic substances from Candida albicans. Infect. Immun 6:616
    [Google Scholar]
  5. Davis B. J. 1964; Disc electrophoresis. II. Method and application to serum proteins. Ann. N. Y. Acad. Sci 121:404
    [Google Scholar]
  6. Douchet C., Müller J. 1972; Valeur et limites de la sérologie du Candida albicans. Lyon Med 227:1129
    [Google Scholar]
  7. Evans E. G. V., Forster R. A. 1976; Antibodies to Candida after operations on the heart. J. med. Microbiol 9:303
    [Google Scholar]
  8. Evans E. G. V., Richardson M. D., Odds F. C., Holland K. T. 1973; Relevance of antigenicity of Candida albicans growth phases to diagnosis of systemic candidiasis. Br. med. J 4:86
    [Google Scholar]
  9. Everall P. H., Morris C. A., Morris D. F. 1974; Antibodies to Candida albicans in hospital patients with and without spinal injury and in normal men and women. J. clin. Path 27:122
    [Google Scholar]
  10. Glew R. H., Buckley H. R., Rosen H. M., Moellering R. C., Fischer J. E. 1975; Value of prospective Candida precipitins in fungaemia in patients with hyperalimentation. Surg. Forum 26:113
    [Google Scholar]
  11. Grabar P., Williams C. A. 1953; Methode permettant l’etude conjugée des propriétés électrophorétiques et immunochimiques d’un mélange de protéines. Application au sérum sanguin. Biochim. biophys. Acta 10:193
    [Google Scholar]
  12. Hotta K., Kurokawa M. 1968; Separation of fucose and acetylhexosamines by two-dimensional thin-layer chromotography. Analyt. Biochem 26:472
    [Google Scholar]
  13. Iwata K. 1977; In Recent advances in medical and veterinary mycology. edited by Iwata K. University Park Press; Baltimore: p 15
    [Google Scholar]
  14. Longbottom J. L., Brighton W. D., Edge G., Pepys J. 1976; Antibodies mediating type 1 skin test reactions to polysaccharide and protein antigens of Candida albicans. Clin. Allergy 6:41
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  16. Müller H. L. 1974; Fortschritte und Probleme der Candidaserologie. Praxis, Berne 63:1210
    [Google Scholar]
  17. Murray I. G., Buckley H. R., Turner G. C. 1969; Serological evidence of Candida infection after open-heart surgery. J. med. Microbiol 2:463
    [Google Scholar]
  18. Oblack D., Schwarz J., Holder I. A. 1976; Comparative evaluation of the Candida agglutinin test, precipitin test and germ tube dispersion test in the diagnosis of candidiasis. J. clin. Microbiol 3:175
    [Google Scholar]
  19. Odds F. C. 1979; Candida and candidosis. Leicester University Press; Leicester: p 224
    [Google Scholar]
  20. Odds F. C., Evans E. G. V., Holland K. T. 1975; Detection of Candida precipitins. A comparison of double diffusion and counter immunoelectrophoresis. J. immunol. Meth 7:211
    [Google Scholar]
  21. Odds F. C., Hierholzer J. C. 1973; Purification and properties of a glycoprotein acid phosphatase from Candida albicans. J. Bact 114:257
    [Google Scholar]
  22. Odds F. C., Milne L. J. R., Gentles J. C., Ball E. H. 1980; The activity in vitro and in vivo of a new imidazole antifungal, ketoconazole. J. antimicrob. Chemother 6:97
    [Google Scholar]
  23. Parsons E. R., Nassau E. 1974; Candida serology in open heart surgery. J. med. Microbiol 7:415
    [Google Scholar]
  24. Pepys J., Faux J. A., Longbottom J. L., McCarthy D. S., Hargreave F. E. 1968; Candida albicans precipitins in respiratory disease in man. J. Allergy 41:305
    [Google Scholar]
  25. Remold H., Fasold H., Staib F. 1968; Purification and characterization of a proteolytic enzyme from Candida albicans. Biochim. Biophys. Acta 167:399
    [Google Scholar]
  26. Spiro R. G. 1966; Analysis of sugars found in glycoproteins. In Methods in enzymology vol 8: edited by Neufeld E. F. Ginsburg V. Academic Press; New York: p 3
    [Google Scholar]
  27. Staib F. 1969; Proteolysis and pathogenicity of Candida albicans strains. Mycopath. Mycol. appl 37:345
    [Google Scholar]
  28. Staib F., Focking M., Fröhlich B., Blisse A. 1977a; Candida albicans—Serumpräzipitine bei Blutspendem: Auffällige serologische und kulturelle. Candida albicans—Befunde ber alkoholikern. Mykosen 20:423
    [Google Scholar]
  29. Staib F., Mishra S. K., Abel T. L. 1977b; Serodiagnostic value of extracellular antigens of an actively proteolysing culture of Candida albicans. Zentbl. Bakt. ParasitKde I. Abt. OrigGinsburg 238:284
    [Google Scholar]
  30. Stanley V. C., Hurley R., Carroll C. J. 1972; Distribution and significance of Candida precipitins in sera from pregnant women. J. med. Microbiol 5:313
    [Google Scholar]
  31. Stickle D., Kaufman L., Blumer S. O., McLaughlin D. W. 1972; Comparison of a newly developed latex agglutination test and an immunodiffusion test in the diagnosis of systemic candidiasis. Appl. Microbiol 23:490
    [Google Scholar]
  32. Syverson R. E., Buckley H. R. 1977; Cell wall antigens in soluble cytoplasmic extracts of Candida albicans as demonstrated by crossed immuno-affinoelectrophoresis with concanavalin A. J. Immunol. Meth 18:149
    [Google Scholar]
  33. Syverson R. E., Buckley H. R., Campbell C. C. 1975; Cytoplasmic antigens unique to the mycelial or yeast phase of Candida albicans. Infect. Immun 12:1184
    [Google Scholar]
  34. Syverson R. E., Buckley H. R., Gibian J. R. 1978; Increasing the predictive value positive of the precipitin test for the diagnosis of deep-seated candidiasis. Am. J. clin. Path 70:826
    [Google Scholar]
  35. Taschdjian C. L., Seelig M. S., Kozinn P. J. 1973; Serological diagnosis of candidal infections. Crit. Rev. clin. lab. Sci 4:19
    [Google Scholar]
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