1887

Abstract

SUMMARY

An antiserum-agar technique was evaluated as a method for detecting in faeces. Thirty-one laboratory strains of produced immunoprecipitate haloes during overnight growth on SS agar and blood-agar-base infusion agar (BAB) containing donkey antiserum to a vaccine strain of Other salmonella species sharing O serogroup antigens with also produced haloes when streaked in pure culture on SS-antiserum agar but not on BAB-antiserum agar. One hundred and forty-one consecutive faecal specimens were cultured on SS-antiserum agar. Results with this method were concordant with those of established isolation techniques on specimens from six of seven suspected carriers of

Ten other salmonellas were isolated from the faecal specimens but only like a serogroup-D organism, yielded false-positive haloes on antiserum agar. The antiserum-agar technique offers promise as a means of screening for in faecal cultures.

Penelope Fox provided expert technical assistance and Inelle Reynolds valuable secretarial service. Carl E. Frasch contributed several helpful insights. We are particularly indebted to John C. Feeley, Center for Disease Control, Atlanta, Georgia, for determining salmonella-antibody titres on the strain Ty 2 antiserum.

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1980-05-01
2022-10-03
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