RT Journal Article SR Electronic(1) A1 Stearne, Lorna E. T. A1 Birkbeck, T. H.YR 1980 T1 The action of formaldehyde on staphylococcal δ haemolysin JF Journal of Medical Microbiology, VO 13 IS 2 SP 223 OP 230 DO https://doi.org/10.1099/00222615-13-2-223 PB Microbiology Society, SN 1473-5644, AB Summary Native staphylococcal δ haemolysin was poorly immunogenic in the mouse. After treatment with formaldehyde at pH 5 or pH 7.5, haemolytic activity was rapidly lost but antigenic reactivity retained and, at pH 5, im-munogenicity was enhanced. Treatment of the haemolysin with formaldehyde at pH 9.5 reduced haemolytic activity by 97% but did not enhance immunogenicity. There was no evidence of polymerisation of formaldehyde-treated δ haemolysin, and the enhanced immunogenicity was considered to be partly due to a reduced affinity for phospholipids., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-13-2-223