Skip to content
1887

Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 13, Issue 1

ISOLATION OF DANE PARTICLES CONTAINING A DNA STRAND BY METRIZAMIDE DENSITY GRADIENT No Access

Abstract

SUMMARY

Dane-particle cores labelled with [H]TTP were subjected to ultracentrifugation in a metrizamide density gradient. Two populations of core particles with different densities were obtained, and radioactivity was found only in the cores that sedimented at the lower density (1.19–1.23 g/cm). All the cores in this group, when spread in a monolayer, were found to expel a closed circular double-stranded DNA molecule. In contrast, the core particles that sedimented at the higher density (1.23–1.27 g/cm) were not associated with radioactivity, nor was any DNA strand extruded from them. These results show that metrizamide density gradients allow the separation of complete hepatitis B virions for the study of viral DNA.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1980
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-13-1-163
1980-02-01
2025-04-21
Loading full text...

Full text loading...

References

  1. Almeida J. D., Rubenstein D., Stott E. J. 1971; New antigen-antibody system in australia-antigen-positive hepatitis. Lancet 2:1225
     [Google Scholar]
  2. Birnie G. D., Rickwood D., Hell A. 1973; Buoyant densities and hydration of nucleic acids, proteins and nucleoprotein complexes in metrizamide. Biochim. Biophys. Acta 331:283
     [Google Scholar]
  3. Dane D. S., Cameron C. H., Briggs M. 1970; Virus-like particles in serum of patients with australia-antigen-associated hepatitis. Lancet 1:695
     [Google Scholar]
  4. Imai M., Tachibana F. C., Moritsugu Y., Miyakawa Y., Mayumi M. 1976; Hepatitis b antigen-associated deoxyribonucleic acid polymerase activity and e antigen/anti-e system. Infect. Immun 14:631
     [Google Scholar]
  5. Kaplan P. M., Greenman R. L., Gerin J. L., Purcell R. H., Robinson W. S. 1973; Dna polymerase associated with human hepatitis b antigen. J. Virol 12:995
     [Google Scholar]
  6. Kaplan P. M., Ford E. C., Purcell R. H., Gerin J. L. 1976; Demonstration of subpopulations of dane particles. J. Virol 17:885
     [Google Scholar]
  7. Kleinschmidt A. K. 1968; Monolayer techniques in electron microscopy of nucleic acid molecules. In methods of enzymology edited by Colowick S. P., Kaplan N. O. Academic press new york: vol Xllb: p 361
     [Google Scholar]
  8. Magnius L. O., Espmark J. A. 1972; New specificities in australia antigen positive sera distinct from le bouvier determinants. J. Immunol 109:1017
     [Google Scholar]
  9. Moritsugu Y., Gold J. W. M., Wagner J., Dodd R. Y., Purcell R. H. 1975; Hepatitis b core antigen. Detection of antibody by radioimmuno-precipitation. J. Immunol 114:1792
     [Google Scholar]
  10. Nordenfeld E., Kjellen L. 1975; Dane particles, dna polymerase, and e-antigen in two different categories of hepatitis b antigen carriers. Intervirology 5:225
     [Google Scholar]
  11. Takahashi K., Imai M., Tsuda F., Takahashi T., Miyakawa Y., Mayumi M. 1976; Association of dane particles with e antigen in the serum of asymptomatic carriers of hepatitis b surface antigen. J. Immunol 117:102
     [Google Scholar]
  12. Takahashi T., Nakagawa S., Hashimoto T., Takahashi K., Imai M., Miyakawa Y., Mayumi M. 1976; Large-scale isolation of dane particles from plasma containing hepatitis b antigen and demonstration of a circular double-stranded dna molecule extruding directly from their cores. J. Immunol 117:1392
     [Google Scholar]
  13. Tsuda F., Takahashi T., Takahashi K., Miyakawa Y., Mayumi M. 1975; Determination of antibody to hepatitis b core antigen by means of immune adherence hemagglutination. J. Immunol 115:834
     [Google Scholar]
/content/journal/jmm/10.1099/00222615-13-1-163
Loading
ISOLATION OF DANE PARTICLES CONTAINING A DNA STRAND BY METRIZAMIDE DENSITY GRADIENT
J. Med. Microbiol. 13, 163 (1980); https://doi.org/10.1099/00222615-13-1-163
/content/journal/jmm/10.1099/00222615-13-1-163
/content/journal/jmm/10.1099/00222615-13-1-163
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error