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Volume 13, Issue 1

ISOLATION OF DANE PARTICLES CONTAINING A DNA STRAND BY METRIZAMIDE DENSITY GRADIENT Free

Abstract

SUMMARY

Dane-particle cores labelled with [H]TTP were subjected to ultracentrifugation in a metrizamide density gradient. Two populations of core particles with different densities were obtained, and radioactivity was found only in the cores that sedimented at the lower density (1.19–1.23 g/cm). All the cores in this group, when spread in a monolayer, were found to expel a closed circular double-stranded DNA molecule. In contrast, the core particles that sedimented at the higher density (1.23–1.27 g/cm) were not associated with radioactivity, nor was any DNA strand extruded from them. These results show that metrizamide density gradients allow the separation of complete hepatitis B virions for the study of viral DNA.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1980
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1980-02-01
2023-03-21
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ISOLATION OF DANE PARTICLES CONTAINING A DNA STRAND BY METRIZAMIDE DENSITY GRADIENT
J. Med. Microbiol. 13, 163 (1980); https://doi.org/10.1099/00222615-13-1-163
/content/journal/jmm/10.1099/00222615-13-1-163
/content/journal/jmm/10.1099/00222615-13-1-163
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