1887

Abstract

SUMMARY

Pathogenic, and laboratory-derived non-adherent, non-motile, streptomycin-dependent and attenuated strains of , were injected into the ileal loops of adult rabbits. The pattern of bacterial adherence and multiplication was studied. It was shown that all the strains multiplied to the same extent in the intestine; multiplication had no role in pathogenesis except when the infecting dose was low.

Vibrio strains differed in their capacity to adhere to the intestine. A good correlation was found between adherence and pathogenesis. While adhesive strains were pathogenic, the poorly adhesive strains proved to be poor pathogens. There was no trace of toxin in the ileal loops inoculated with poorly adhesive strains and very little in the diarrhoea1 fluid produced by pathogenic strains. Adhesive strains adhered poorly to the intestine of immunised animals. It is suggested that adherence is concerned in the release of toxin and thus plays an important role in the pathogenesis of cholera.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-13-1-1
1980-02-01
2022-08-14
Loading full text...

Full text loading...

/deliver/fulltext/jmm/13/1/medmicro-13-1-1.html?itemId=/content/journal/jmm/10.1099/00222615-13-1-1&mimeType=html&fmt=ahah

References

  1. Adler J. 1969; Chemoreceptors in bacteria. Science 166:1588
    [Google Scholar]
  2. Adler J. 1973; A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J. gen. Microbiol 74:77
    [Google Scholar]
  3. Allweiss B., Dostal J., Carey K. E., Edwards T. F., Freter R. 1977; The role of chemotaxis in the ecology of bacterial pathogens of mucosal surfaces. Nature, Lond 266:448
    [Google Scholar]
  4. Aziz K. M. S., Mosley W. H. 1972; Quantitative studies of toxin in the stools and jejunal aspirates of patients with cholera. J. infect. Dis 125:36
    [Google Scholar]
  5. Bhaskaran K., Sinha V. B. 1967; Attenuation of virulence in Vibrio cholerae. J. Hyg., Camb 65:135
    [Google Scholar]
  6. Bhattacharjee J. W., Srivastava B. S. 1979; Adherence of wild-type and mutant strains of Vibrio cholerae to normal and immune intestinal tissue. Bull. Wld Hlth Org 57:123
    [Google Scholar]
  7. Craig J. O. 1965; The effect of cholera stool and culture filtrates on the skin of guinea pigs and rabbits. In Proceedings of the cholera research symposium edited by Bushnell O. A., Brookhyser C. S. US Govt Printing Office: Washington, DC: p 153
    [Google Scholar]
  8. De S. N., Chatterje D. N. 1953; An experimental study of the mechanism of action of Vibrio cholerae on the intestinal mucous membrane. J. Path. Bact 66:559
    [Google Scholar]
  9. Dixon J. M. S. 1960; The fate of bacteria in the small intestine. J. Path. Bact. 79:131
    [Google Scholar]
  10. Finkelstein R. A. 1975; Immunology of cholera. Curr. Top. Microbiol. Immun 69:138
    [Google Scholar]
  11. Finkelstein R. A., LoSpalluto J. J. 1969; Pathogenesis of experimental cholera: preparation and isolation of choleragen and choleragenoid. J. exp. Med 130:185
    [Google Scholar]
  12. Freter R. 1969; Studies of the mechanism of action of intestinal antibody in experimental cholera. Tex. Rep. Biol. Med. 27: suppl 1299
    [Google Scholar]
  13. Freter R., Jones G. W. 1976; Adhesive properties of Vibrio cholerae: nature of the interaction with intact mucosal surfaces. Infect. Immun 14:246
    [Google Scholar]
  14. Guentzel M. N., Berry L. J. 1975; Motility as a virulence factor for Vibrio cholerae. Infect. Immun 11:890
    [Google Scholar]
  15. Labrec E. H., Sprinz H., Schneider H., Formal S.B. 1965; Localization of vibrios in experimental cholera: a fluorescent antibody study in guinea pigs. In Proceedings of the cholera research symposium edited by Bushnell O. A., Brookhyser C. S. US Govt Printing Office:Washington, DC: p 272
    [Google Scholar]
  16. Nelson E. T., Clements J. D., Finkelstein R. A. 1976; Vibrio cholerae adherence and colonization in experimental cholera: electron microscopic studies. Infect. Immun 14:527
    [Google Scholar]
  17. Patnaik B. K., Ghosh H. K. 1966; Histopathological studies on experimental cholera. Br. J. exp. Path 47:210
    [Google Scholar]
  18. Schrank G. D., Verwey W. F. 1976; Distribution of cholera organisms in experimental Vibrio cholerae infections: proposed mechanism of pathogenesis and antibacterial immunity. Infect. Immun 13:195
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-13-1-1
Loading
/content/journal/jmm/10.1099/00222615-13-1-1
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error