1887

Abstract

SUMMARY

We examined the results of tests with 22 substrates for their ability to discriminate a series of 917 strains of collected from different sources. The tests with three of the substrates were discarded because of difficulties in performance or interpretation, and another nine substrates because they provided little discrimination. The tests used to obtain biotype profiles for strains were those for the fermentation of dulcitol, D-raffinose or sucrose or both, L-rhamnose and L-sorbose, the decarboxylation of L-lysine and L-ornithine, the hydrolysis of aesculin, motility, and prototrophy.

Observations on several series of cultures from different sources showed that biotype characters were stable and after storage on non-selective medium. The biotype profiles obtained were as reliable as partial O serotyping for the routine subtyping of strains of isolated from the urine of patients with long-term urinary-tract infections and those from other sources in different patients. Biotyping and O serotyping used in conjunction offered a very fine degree of strain discrimination.

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/content/journal/jmm/10.1099/00222615-12-4-473
1979-11-01
2024-12-12
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References

  1. Arr M., Perenyi T., Novak E. K. 1970; Sucrose and raffinose breakdown by Escherichia coli. Acta microhiol. hung 17:117
    [Google Scholar]
  2. Bettelheim K. A., Taylor J. 1969; A study of Escherichia coli isolated from chronic urinary infection. J. med. Microbiol 2:225
    [Google Scholar]
  3. Bettelheim K. A., Teoh-Chan C. H., Chandler M. E., O’Farrell S. M., Rahamin L., Shaw E. J., Shooter R. A. 1974; Further studies of Escherichia coli in babies after normal delivery. J. Hyg. Camb 73:277
    [Google Scholar]
  4. Buissiere J., Coynault C., Le Minor L. 1977; Etude des conditions d’expression du caractere raffinose chez les Escherichia coli et Salmonella. Annali Microbiol 128:167
    [Google Scholar]
  5. Butler D. A., Lobregat C. M., Gavan T. L. 1975; Reproducibility of the Analytab (API 20E) system. J. clin. Microbiol 2:322
    [Google Scholar]
  6. Cornelis G., Luke R. K. J., Richmond M. H. 1978; Fermentation of raffinose by lactose-fermenting strains of Yersinia enterocolitica and by sucrose-fermenting strains of Escherichia coli. J. clin. Microbiol. 7:180
    [Google Scholar]
  7. Cowan S. T. 1974 Cowan and Steel’s manual for the identification of medical bacteria 2nd ed Cambridge:
    [Google Scholar]
  8. Davies B. I. 1977; Biochemical typing of urinary Escherichia coli strains by means of the API 20E Enterobacteriaceae system. J. med. Microbiol 10:293
    [Google Scholar]
  9. Duguid J. P., Anderson E. S., Alfredsson G. A., Barker R., Old D. C. 1975; A new biotyping scheme for Salmonella typhimurium and its phylogenetic significance. J. med. Microbiol 8:149
    [Google Scholar]
  10. Edberg S. C., Pittman S., Singer J. M. 1977; Esculin hydrolysis by Enterobacteriaceae. J. clin. Microbiol 6:111
    [Google Scholar]
  11. Elek S. D., Higney L. 1970; Resistogram typing—a new epidemiological tool: application to Escherichia coli. J. med. Microbiol 3:103
    [Google Scholar]
  12. Magalhaes M., Vance M. 1978; Hydrogen sulphide-positive strains of Escherichia coli from swine. J. med. Microbiol 11:211
    [Google Scholar]
  13. Meynell G. G., Meynell E. 1965 Theory and practice in experimental bacteriology 1 st ed Cambridge:
    [Google Scholar]
  14. Miskin A., Edberg S. C. 1978; Esculin hydrolysis reaction by Escherichia coli. J. clin. Microbiol 7:251
    [Google Scholar]
  15. Mushin R., Ashburner F. M. 1964; Ecology and epidemiology of coliform infections: II. The biochemical reactions and drug sensitivity of coliform organisms. Med. J. Aust 1:303
    [Google Scholar]
  16. Sakazaki R., Tamura K., Nakamura A. 1974; Further studies on enteropathogenic Escherichia coli associated with diarrhoeal diseases in children and adults. Jap. J. med. Sci. Biol 27:7
    [Google Scholar]
  17. Van derWaaij D., Speltie T. M., Guin£e P. A. M., Agterberg C. 1975; Serotypingand biotyping of 160 Escherichia coli strains: comparative study. J. clin. Microbiol 1:237
    [Google Scholar]
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