1887

Abstract

Summary

A scheme for the “fingerprinting” of streptococci according to their production of (P typing) and sensitivity to (S typing) bacteriocine-like inhibitory substances has been developed.

P typing of 450 β-haemolytic streptococci by their action on a set of nine standard indicator strains revealed that 80% of strains produced one or more detectable inhibitors, and that 17 different P types could be recognised. Production of some inhibitors seemed to be a property of strains of a particular serological group or type. Bacteriocine-like substances were produced by streptococci of serological groups, A, B, C, D, E, F and G.

Nine strains were selected as standard producers for S typing. These strains differed in their spectra of inhibition, but all seemed to be active only against gram-positive bacteria. One producer, a group-F streptococcus, specifically inhibited group-A streptococci.

The conditions of incubation were critical for demonstration of inhibitor production. A requirement for blood and for incubation at 32°C were important factors. None of the inhibitors was induced by ultraviolet irradiation. The observed inhibitory effects were not attributable to either hydrogen peroxide or low/H, but to the production of a variety of substances having diverse physicochemical properties and production requirements. Most of the inhibitors do not seem to be produced in liquid media.

The “fingerprinting” procedure is simple and inexpensive, and provides a reliable means of subdividing streptococcal strains that may find application as a supplement to the existing serological typing schemes.

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1979-11-01
2022-05-28
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References

  1. Abbott J. D., Shannon R. 1958; A method for typing Shigella sonnei, using colicine production as a marker. J. clin. path 11:71
    [Google Scholar]
  2. Al-Jumaili I. J. 1975; Bacteriocine typing of Proteus. J. clin. Path 28:784
    [Google Scholar]
  3. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bact. Rev 31:230
    [Google Scholar]
  4. Brock T. D., Peacher B., Pierson D. 1963; Survey of the bacteriocins of enterococci. J. Bact 86:702
    [Google Scholar]
  5. Cohen J. O. 1969; Effect of culture medium composition and pH on the production of M protein and proteinase by group A streptococci. J. Bact 99:737
    [Google Scholar]
  6. Dajani A. S., Veres C. M. 1977; Inactivation of a streptococcal bacteriocin (viridin B) by mammalian hemoglobin. Proc. Soc. exp. Biol. Med 155:456
    [Google Scholar]
  7. Darrell J. H., Wahba A. H. 1964; Pyocine-typing of hospital strains of Pseudomonas pyocyanea. J. clin. Path 17:236
    [Google Scholar]
  8. Elliott S. D. 1945; A proteolytic enzyme produced by group A streptococci with special reference to its effect on the type-specific M antigen. J. exp. Med 81:573
    [Google Scholar]
  9. Farkas-Himsley H., Pagel A. 1977; Bacteriocin typing by leakage of ultraviolet lightabsorbing material. Infect. Immun 16:12
    [Google Scholar]
  10. Farmer J. J. 1972; Epidemiological differentiation of Serratia marcescens: typing by bacteriocin sensitivity. Appl. Microbiol 23:226
    [Google Scholar]
  11. Farmer J. J., Herman L. G. 1969; Epidemiological fingerprinting of Pseudomonas aeruginosa by the production of and sensitivity to pyocin and bacteriophage. Appl. Microbiol 18:760
    [Google Scholar]
  12. Gillies R. R., Govan J. R. W. 1966; Typing of Pseudomonas pyocyanea by pyocine production. J. Path. Bact 91:339
    [Google Scholar]
  13. Hardy K. G. 1975; Colicinogeny and related phenomena. Bact. Rev 39:464
    [Google Scholar]
  14. Johnson D. W., Tagg J. R., Wannamaker L. W. 1979; Production of a bacteriocine-like substance by group-A streptococci of M-type 4 and T-pattern 4. J.med. Microbiol 12:413
    [Google Scholar]
  15. Kékessy D. A., Piguet J. D. 1971; Bactériocinogénie et typisation de Streptococcus faecalis. Pathologia Microbiol 37:113
    [Google Scholar]
  16. Kelstrup J., Gibbons R. J. 1969; Bacteriocins from human and rodent streptococci. Archs oral Biol 14:251
    [Google Scholar]
  17. Kohn J. 1966; Modified procedure for pyocine typing. J. clin. Path 19:403
    [Google Scholar]
  18. Kolesnichenko T. G., Totolyan A. A. 1971; Distribution of bacteriocin production among group A hemolytic streptococci. Bull. exp. Biol. Med 71:661
    [Google Scholar]
  19. Kramer J., Brandis H. 1972; Charakterisierung eines Streptococcus agalactiae- Bacteriocins. Zentbl Bakt. ParasitKde I Abt. Orig A219:290
    [Google Scholar]
  20. Kuttner A. G. 1966; Production of bacteriocines by group A streptococci with special reference to the nephritogenic types. J. exp. Med 124:279
    [Google Scholar]
  21. Lütticken R., Lütticken D., Johnson D. R., Wannamaker L. W. 1976; Application of a new method for detecting streptococcal nicotinamide adenine dinucleotide glycohydro-lase to various M types of Streptococcus pyogenes. J. clin. Microbiol 3:533
    [Google Scholar]
  22. Malke H., Starke R., Jacob H. E., Kohler W. 1974; Bacteriocine-like activity of group-A streptococci due to the production of peroxide. J. med. Microbiol 7:367
    [Google Scholar]
  23. Overturf G. D., Mortimer E. A. 1970; Studies of the relationship between the production o, f bacteriocines by group A streptococci and acute glomerulonephritis. J. exp. Med 132:694
    [Google Scholar]
  24. Pleceaš P., Bogdan C., Vereanu A. 1972; Enterocine-typing of group D streptococci. Zentbl Bakt. ParasitKde I Abt. Orig A221:173
    [Google Scholar]
  25. Prakash K., Ravindran P. C., Sharma K. B. 1973; Production of streptocines by beta haemolytic streptococci isolated from human sources. Indian J. med. Res 61:1261
    [Google Scholar]
  26. Rogers A. H. 1976; Bacteriocinogeny and the properties of some bacteriocins of Streptococcus mutans. Archs oral Biol 21:99
    [Google Scholar]
  27. Senior B. W. 1977; Typing of Proteus strains by proticine production and sensitivity. J. med. Microbiol 10:7
    [Google Scholar]
  28. Sherwood N. P., Russell B. E., Jay A. R., Bowman K. 1949; Studies on streptococci. III. New antibiotic substances produced by beta hemolytic streptococci. J. infect. Dis 84:88
    [Google Scholar]
  29. Tagg J. R., Dajani A. S., Wannamaker L. W. 1975; Bacteriocin of a group B streptococcus: partial purification and characterization. Antimicrob. Agents Chemother 7:764
    [Google Scholar]
  30. Tagg J. R., Dajani A. S., Wannamaker L. W. 1976; Bacteriocins of gram-positive bacteria. Bact. Rev 40:722
    [Google Scholar]
  31. Tagg J. R., Dajani A. S., Wannamaker L. W., Gray E. D. 1973b; Group A streptococcal bacteriocin. Production, purification and mode of action. J. exp. Med 138:1168
    [Google Scholar]
  32. Tagg J. R., McGiven A. R. 1972; Some possible autoimmune mechanisms in rheumatic carditis. Lancet 2:686
    [Google Scholar]
  33. Tagg J. R., Mushin R. 1971; Epidemiology of Pseudomonas aeruginosa infection in hospitals. I. Pyocine typing of Ps. aeruginosa. Med. J. Aust 1:847
    [Google Scholar]
  34. Tagg J. R., Mushin R. 1973; Pyocin-sensitivity testing as a means of typing Pseudomonas aeruginosa. J. med. Microbiol 6:559
    [Google Scholar]
  35. Tagg J. R., Read R. S. D., McGiven A. R. 1973a; Bacteriocin of a group A streptococcus: partial purification and properties. Antimicrob. Agents Chemother 4:214
    [Google Scholar]
  36. Tagg J. R., Wannamaker L. W. 1976; Genetic basis of streptococcin A-FF22 production. Antimicrob. Agents Chemother 10:299
    [Google Scholar]
  37. Tripathy G. S., Chadwick P. 1971; The effect of mitomycin C on the pyocine typing patterns of hospital strains of Pseudomonas aeruginosa. Can. J. Microbiol 17:829
    [Google Scholar]
  38. Tzannetis S., Leonardopoulos J., Papavassiliou J. 1970; Enterocinogeny and lysogeny in enterococci. J. appl. Bact 33:358
    [Google Scholar]
  39. Tzannetis S., Poulaki-Tsontou A., Papavassiliou J. 1974; Bacteriocine production in group B streptococci. Pathologia Microbiol 41:51
    [Google Scholar]
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