1887

Journal of Medical Microbiology logo

Volume 12, Issue 3

DIFFERENTIATION OF FLUORESCENT PSEUDOMONADS BY THEIR EFFECT ON MILK AGAR Free

Abstract

SUMMARY

Eighty-six clinical isolates of fluorescent pseudomonads that did not produce pyocyanin on Diagnostic Sensitivity Test Agar or Cetrimide Agar were identified on the basis of their antibiotic sensitivity, production of pigment on King’s “A” medium, growth at 42°C, production of lecithinase and hydrolysis of gelatin. The identity of the strains was confirmed in tests with the ammonium salt sugars ethanol, glucose and mannitol. These tests were adequate for distinguishing between the three important fluorescent pseudomonads.

The detection of casein hydrolysis on milk agar was assessed as a rapid method of distinguishing from the other species of fluorescent pseudomonads but proved unhelpful when compared with, or included in, a small set of tests. Most strains of and hydrolysed casein.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1979
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-12-3-379
1979-08-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jmm/12/3/medmicro-12-3-379.html?itemId=/content/journal/jmm/10.1099/00222615-12-3-379&mimeType=html&fmt=ahah

References

  1. Brown M. R. W., Scott-Foster J. H. 1970; A simple diagnostic milk medium for Pseudomonas aeruginosa. J. clin. Path 23:172
     [Google Scholar]
  2. Falkiner F. R., Keane C. T., Taylor M. R. H. 1974; Contamination of urine specimens with Pseudomonasputida and Pseudomonas fluorescens. Proc. Soc. gen. Microbiol 2:15
     [Google Scholar]
  3. Gilardi G. L. 1972; Infrequently encountered Pseudomonas species causing infection in humans. Ann. intern. Med 77:211
     [Google Scholar]
  4. Gilardi G. L. 1976; Pseudomonas species in clinical microbiology. Mt Sinai J. Med 43:710
     [Google Scholar]
  5. King A., Phillips I. 1978; The identification of pseudomonads and related bacteria in a clinical laboratory. J. med. Microbiol 11:165
     [Google Scholar]
  6. King E. O., Ward M. K., Raney D. E. 1954; Two simple media for the demonstration of pyocyanin and fluorescin. J. lab. clin. Med 44:301
     [Google Scholar]
  7. Martin W. J., Maker M. D., Washington J. A. 1973; Bacteriology and in vitro antimicrobial susceptibility of the Pseudomonas fluorescens group isolated from clinical specimens. Am. J. clin. Path 60:831
     [Google Scholar]
  8. Phillips I. 1969; Identification of Pseudomonas aeruginosa in the clinical laboratory. J. med. Microbiol 2:9
     [Google Scholar]
/content/journal/jmm/10.1099/00222615-12-3-379
Loading
DIFFERENTIATION OF FLUORESCENT PSEUDOMONADS BY THEIR EFFECT ON MILK AGAR
J. Med. Microbiol. 12, 379 (1979); https://doi.org/10.1099/00222615-12-3-379
/content/journal/jmm/10.1099/00222615-12-3-379
/content/journal/jmm/10.1099/00222615-12-3-379
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error