1887

Abstract

SUMMARY

Inhibitors of trophozoite motility and phagocytosis were used to investigate the mechanism of cytopathogenicity in mouse-embryo (ME)-cell cultures. Amoebae that were immobilised and agglutinated by specific antiserum exhibited no cytopathic activity, although they remained alive and were in constant contact with the ME cells. Mammalian-cell damage occurred only when the organisms recovered pseudopodium function and began to migrate over the monolayers as they overcame the inhibitory effects of the antiserum. Cytochalasin B at a concentration of 10 μg/ml, shown to prevent the engulfment of chick erythrocytes by amoebae, also inhibited the cytopathogenicity of when incorporated in ME-cell culture medium. Despite repeated contact with active trophozoites, the ME cells showed only those morphological changes characteristically induced by cytochalasin B itself. The amoebae in turn showed signs of starvation after 3 or 4 days’ incubation, suggesting that the feeding activity of trophozoites was suppressed. Colchicine, on the other hand, inhibited neither the ingestion of erythrocytes nor the destruction of ME cells by amoebae. It was concluded that the cytopathogenicity of in ME-cell cultures was due to physical rather than biochemical or cytotoxic mechanisms and was associated with the phagocytic activity of trophozoites.

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1979-08-01
2024-05-26
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References

  1. Allison A. C., Davies P., de Petris S. 1971; Role of contractile microfilaments in macrophage movement and endocytosis. Nature, New Biol 232:153
    [Google Scholar]
  2. Anderson K., Jamiesoan A. 1972; Agglutination test for the investigation of the genus Naegleria. Pathology, Sydney 4:273
    [Google Scholar]
  3. Axline S. G., Reaven E. 1972; In vitro inhibition of phagocytosis and disruption of microfilaments by cytochalasin B. J. clin. Invest 51:6a
    [Google Scholar]
  4. Bhisey A. N., Freed J. J. 1971; Altered movement of endosomes in colchicine-treated cultured macrophages. Expl Cell Res 64:430
    [Google Scholar]
  5. Biagi F. F., Beltran H. F. 1969; The challenge of amebiasis: understanding pathogenic mechanisms. Int. Rev. trop. Med 3:219
    [Google Scholar]
  6. Brown T. 1978; Observations by light microscopy on the cytopathogenicity of Naegleria fowleri in mouse embryo-cell cultures. J. med. Microbiol 11:249
    [Google Scholar]
  7. Carter R. F. 1968; Primary amoebic meningo-encephalitis: clinical, pathological and epidemiological features of six fatal cases. J. Path. Bact 96:1
    [Google Scholar]
  8. Carter R. F. 1970; Description of a Naegleria sp. isolated from two cases of primary amoebic meningo-encephalitis and of the experimental pathological changes induced by it. J. Path 100:217
    [Google Scholar]
  9. Carter R. F. 1972; Primary amoebic meningo-encephalitis. An appraisal of present knowledge. Trans. R. Soc. trop. Med. Hyg 66:193
    [Google Scholar]
  10. Carter S. B. 1967; Effects of cytochalasins on mammalian cells. Nature, Lond 213:261
    [Google Scholar]
  11. Chang S. L. 1973; Pathogenic free-living amoebae and recreational waters. In Advances in water pollution research edited by Jenkins S. H. Oxford: p 85
    [Google Scholar]
  12. Culbertson C. G. 1971; The pathogenicity of soil amebas. A. Rev. Microbiol 25:231
    [Google Scholar]
  13. Davies P., Fox R. I., Polyzonis M., Allison A. C., Haswell A. D. 1973; The inhibition of phagocytosis and facilitation of exocytosis in rabbit polymorphonuclear leukocytes by cytochalasin B. Lab. Invest 28:16
    [Google Scholar]
  14. Duma R. J. 1972; Primary amoebic meningoencephalitis. C.R.C. Crit. Rev. clin. lab. Sci 3:163
    [Google Scholar]
  15. Eaton R. D. P., Meerovitch E., Costerton J. W. 1969; A surface-active lysosome in Entamoeba histolytica. Trans. R. Soc. trop. Med. Hyg 63:678
    [Google Scholar]
  16. Eaton R. D. P., Meerovitch E., Costerton J. W. 1970; The functional morphology of pathogenicity in Entamoeba histolytica. Ann. trop. med. Parasit 64:299
    [Google Scholar]
  17. Hartwig J. H., Stossell T. P. 1976; Interactions of actin, myosin, and an actin-binding protein of rabbit pulmonary macrophages. III. Effects of cytochalasin B. J. cell Biol 71:295
    [Google Scholar]
  18. Jarumilinta R., Kradolfer F. 1964; The toxic effect of Entamoeba histolytica on leukocytes. Ann. trop. med. Parasit 58:375
    [Google Scholar]
  19. Krantz G. E. 1969; Acanthamoeba castellanii: specificity of immobilization test. Expl. Parasit 26:299
    [Google Scholar]
  20. Maitra S. C., Krishna Prasad B. N., Das S. R., Agarwala S. C. 1974; Study of Naegleria aerobia by electron microscopy. Trans. R. Soc. trop. Med. Hyg 68:56
    [Google Scholar]
  21. Maitra S. C., Krishna Prasad B. N., Agarwala S. C., Das S. R. 1976; Ultrastructural studies on experimental primary amoebic meningo-encephalitis (PAME) of mouse due to Naegleria aerobia and Hartmannella culbertsoni. Int. J. Parasit 6:489
    [Google Scholar]
  22. Martinez A. J., Duma R. J., Nelson E. C., Moretta F. L. 1973; Experimental naegleria meningoencephalitis in mice. Penetration of the olfactory mucosal epithelium by naegleria and pathological changes produced: a light and electron microscope study. Lab. Invest 29:121
    [Google Scholar]
  23. Newton B. A. 1972; Protozoal pathogenicity. In Microbial pathogenicity in man and animals symposium no. 22, Society for General Microbiology, edited by Smith H., Pearce J. H. Cambridge: p 269
    [Google Scholar]
  24. Novikoff A. B., Holtzman E. 1970; Cells and organelles. New York: pp 150, 152
    [Google Scholar]
  25. Page F. C. 1967; Taxonomic criteria for Umax amoebae, with descriptions of 3 new species of Hartmannella and 3 of Vahlkampfia. J. Protozool 14:499
    [Google Scholar]
  26. Phillips B. P. 1974; Naegleria: another pathogenic ameba. Studies in germfree guinea pigs. Am. J. trop. Med. Hyg 23:850
    [Google Scholar]
  27. Pollard T. D., Weihing R. R. 1974; Actin and myosin and cell movement. C.R.C. crit. Rev. Biochem 2:1
    [Google Scholar]
  28. Reaven E. P., Axline S. G. 1973; Subplasmalemmal microfilaments and microtubules in resting and phagocytizing cultivated macrophages. J. cell. Biol 59:12
    [Google Scholar]
  29. Singh B. N., Das S. R. 1970; Studies on pathogenic and non-pathogenic small free-living amoebae and the bearing of nuclear division on the classification of the order Amoebida. Phil. Trans. R. Soc. B 259:435
    [Google Scholar]
  30. Visvesvara G. S., Callaway C. S. 1974; Light and electron microscopic observations on the pathogenesis of Naegleria fowleri in mouse brain and tissue culture. J. Protozool 21:239
    [Google Scholar]
  31. Weihing R. R. 1976; Cytochalasin B inhibits actin-related gelation of HeLa cell extracts. J. cell Biol 71:303
    [Google Scholar]
  32. Wessells N. K., Spooner B. S., Ash J. F., Bradley M. O., Luduena M. A., Taylor E. L., Wrenn J. T., Yamada K. M. 1971; Microfilaments in cellular and developmental processes. Science, N.Y 171:135
    [Google Scholar]
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