1887

Abstract

SUMMARY

The occurrence and nature of passive protective antibody in 100 samples of human serum was investigated in mice challenged with strains of capsular types A (Smith diffuse strain) and B (strain NS58D). Sixty of the sera passively protected mice against the capsular type-A strain, three against type B, and one against both types. Rabbit antisera against human IgG, IgA and IgM could remove the protective activity from a human serum of high potency, and the activity was also sensitive to 2-mercaptoethanol. Absorption with Smith surface antigen removed protective activity and reduced the concentration of IgG 7-fold, IgA 2.7 fold and of IgM 3-fold more than in a non-protective serum. Consequently, the protective activity of human serum is believed to be associated with antibodies to the capsular antigen in the three immunoglobulin classes.

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/content/journal/jmm/10.1099/00222615-12-3-277
1979-08-01
2023-01-29
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References

  1. Ekstedt R. D., Yoshida K. 1969; Immunity to staphylococcal infection in mice: effect of living versus killed vaccine, role of circulating antibody, and induction of protection-induc ing antigen(s) in vitro. J. Bact 100:745
    [Google Scholar]
  2. Fisher S. 1961; Observations on an anti-staphylococcal mouse protective antibody in human sera. Aust. J. exp. Biol. med. Sci 39:413
    [Google Scholar]
  3. Harasawa I., Minegishi Y., Ohtomo T., Yoshida K. 1975; Capsular typing of Staphylo coccus aureus strains by the fluorescent antibody technique. Jap. J. clin. Path 23:469
    [Google Scholar]
  4. Koenig M. G., Melly M. A., Rogers D. E. 1962; Factors relating to the virulence of staphylococcus. II. Observations on four mouse-pathogenic strains. J. exp. Med 116:589
    [Google Scholar]
  5. Morse S. I. 1962; Isolation and properties of a surface antigen of Staphylococcus aureus. J. exp. Med 115:295
    [Google Scholar]
  6. Rogers D. E. 1962; Staphylococci and man. J. Am. med. Ass 181:38
    [Google Scholar]
  7. Yocum M. W., Strong D. M., Chusid M. J., Lakin J. D. 1976; Selective immunoglobulin M (IgM) deficiency in two immunodeficient adults with recurrent staphylococcal pyoderma. Am. J. Med 60:486
    [Google Scholar]
  8. Yoshida K. 1971; Demonstration of serologically different capsular types among strains of Staphylococcus aureus by the serum-soft agar technique. Infect. Immun 3:535
    [Google Scholar]
  9. Yoshida K. 1972a; Isolation of an additional capsular-type strain of Staphylococcus aureus by the serum-soft agar technique. Infect. Immun 5:833
    [Google Scholar]
  10. Yoshida K. 1972b; Relation of protection inducing activity in mice to capsular type of strains of Staphylococcus aureus. Jap. J. Bacteriol 27:829 (in Japanese)
    [Google Scholar]
  11. Yoshida K., Ekstedt R. D. 1968; Antibody response to Staphylococcus aureus in rabbits: sequence of immunoglobulin synthesis and its correlation with passive protection in mice. J. Bact 96:1540
    [Google Scholar]
  12. Yoshida K., Ichiman Y., Ohtomo T. 1975; Induction of resistance with heat-killed compact-type strains of Staphylococcus aureus against challenge with the diffuse variant of the Smith strain of Staphylococcus aureus. Infect. Immun 12:939
    [Google Scholar]
  13. Yoshida K., Nakamura A., Ohtomo T., Iwami S. 1974; Detection of capsular antigen production in unencapsulated strains of Staphylococcus aureus. Infect. Immun 9:620
    [Google Scholar]
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