species isolated from the mouths of healthy children and of patients with denture stomatitis included strains of that formed germ tubes when incubated in serum.

Twenty-six germ-tube-forming strains of and of were subcultured weekly for 9 wk on blood agar and on Sabouraud's agar and the ability of each subculture to form germ tubes was measured. All the strains of formed almost as many germ tubes after nine weekly subcultures as they did when first isolated. By contrast, although all 26 strains of C. tropicalis formed germ tubes when first isolated, all had lost the ability to do so after six serial weekly subcultures.

Germ-tube formation should not be the sole criterion for the identification of oral strains.


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