1887

Abstract

SUMMARY

The effects of rapid and slow rates of freezing in liquid nitrogen, storage in liquid nitrogen for 12 months, and the rate of subsequent thawing on the viability and growth of in the mouse footpad were studied. Some loss of viability of was detected, and this was found to be associated with the freezing process, rather than with storage or thawing. Slow freezing was less deleterious than quick freezing, with a loss of viability of 90% compared with 98%. The growth pattern of was unaffected except for a delay in the appearance of growth caused by the loss of viability, though there was some evidence of an increased lag phase of one strain, possibly due to the repair of sublethally damaged organisms.

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/content/journal/jmm/10.1099/00222615-12-1-137
1979-02-01
2022-01-27
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References

  1. Beadle D. J., Harris L. W. 1974; Relationship between freezing rate, ultrastructure and recovery in a human diploid cell line. J. Cell Sci 15:419
    [Google Scholar]
  2. Davies J. D. 1970; The role of peptides in preventing freeze-thaw injury. In The frozen cell, Ciba Foundation Symposium edited by Wolstenholme G. E. W., O’Connor M. London: p 213
    [Google Scholar]
  3. Farrant J., Knight S. C., Morris G. J. 1972; Use of different cooling rates during freezing to separate populations of human peripheral blood lymphocytes. Cryobiology 9:516
    [Google Scholar]
  4. Holmes I. B., Hilson G. R. F. 1972; The effect of rifampicin and dapsone on experimental Mycobacterium leprae infections: minimum inhibitory concentrations and bactericidal action. J. med. Microbiol 5:251
    [Google Scholar]
  5. Levy L. 1969; The effect of several rates of freezing and thawing on the viability of Mycobacterium leprae. Cryobiology 6:42
    [Google Scholar]
  6. Levy L. 1971; The effect of freezing and storage at − 60 °C on the viability of Mycobacterium leprae. Cryobiology 8:574
    [Google Scholar]
  7. Mazur P. 1960; Physical factors implicated in the death of micro-organisms at subzero temperatures. Ann. N. Y. Acad. Sci 85:610
    [Google Scholar]
  8. Pattyn S. R. 1973; Conservation of Mycobacterium leprae in liquid nitrogen. Annls Soc. beige Med. trop 53:645
    [Google Scholar]
  9. Ray B., Speck M. L. 1973; Freeze injury in bacteria. C.R.C., Crit. Rev. clin. lab. Sci. 4:161
    [Google Scholar]
  10. Shepard C. C. 1967; A kinetic method for the study of activity of drugs against Mycobacterium leprae in mice. Int. J. Lepr 35:429
    [Google Scholar]
  11. Taylor J. 1962; The estimation of numbers of bacteria by tenfold dilution series>. J. applBad 25:54
    [Google Scholar]
  12. Thurston R. J., Harris G. C. Jr 1970; Calcium-45 uptake as a measurement of freeze-thaw injury to chicken spermatozoa in the presence or absence of cryopreservatives. Cryobiology 7:100
    [Google Scholar]
  13. Whittingham D. G., Leibo S. P., Mazur P. 1972; Survival of mouse embryos frozen to −196° and −296°C. Science, N. Y 178:411
    [Google Scholar]
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