1887

Abstract

SUMMARY

Small round viruses (SRV) were isolated from the faeces of diarrhoeic calves from three farms. All three SRV preparations caused diarrhoea experimentally in gnotobiotic calves. Each preparation contained viral particles of two morphological types, “astrovirus-like” and “calicivirus-like”, and from one preparation the two particle types were separated from each other. The calicivirus-like agent (“Newbury agent”) was 33 nm in diameter, and caused diarrhoea in gnotobiotic calves, with villous atrophy and D-xylose malabsorption. This virus did not infect cell cultures. The astrovirus-like agent did not cause diarrhoea in two gnotobiotic calves; however, it infected cell cultures (primary calf kidney) and the infected cells immunofluoresced with convalescent gnotobiotic-calf antiserum. The astrovirus-like agents in the three preparations were antigenically related. Experiments in calves showed that there was a degree of cross-protection between the three SRV preparations, as judged by the presence or absence of diarrhoea, but that at least three unrelated pathogens were present.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-11-4-441
1978-11-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/11/4/medmicro-11-4-441.html?itemId=/content/journal/jmm/10.1099/00222615-11-4-441&mimeType=html&fmt=ahah

References

  1. Acres S. D., Laing C. J., Saunders J. R., Radistits O. M. 1975; Acute undifferentiated neonatal diarrhoea in beef calves. 1. Occurrence and distribution of infectious agents. Can. J. comp. Med 39:116
    [Google Scholar]
  2. Almeida J. D., Waterson A. P., Prydie J., Fletcher E. W. L. 1968; The structure of a feline picornavirus, and its relevance to cubic viruses in general. Arch. ges. Virus-forsch 25:105
    [Google Scholar]
  3. Bridger J. C., Woode G. N. 1975; Neonatal calf diarrhoea: identification of reovirus-like (rotavirus) agent in faeces by immunofluorescence and immune electron microscopy. Br. vet. J 131:528
    [Google Scholar]
  4. Bridger J. C., Woode G. N., Meyling A. 1978; Isolation of coronaviruses from neonatal calf diarrhoea in Great Britain and Denmark. Vet. Microbiol in press
    [Google Scholar]
  5. Crouch C. F., Woode G. N. 1978; Serial studies of virus multiplication and intestinal damage in gnotobiotic piglets infected with rotavirus. J. med. Microbiol in press
    [Google Scholar]
  6. Dennis M. J., Davies D. C., Hoare M. N. 1976; A simplified apparatus for the microbiological isolation of calves. Br. vet. J 132:642
    [Google Scholar]
  7. Flewett T. H., Woode G. N. 1978; The rotaviruses. Archs Virol 56: in press
    [Google Scholar]
  8. Hill F. W. G., Kidder D. E., Frew J. F. 1970; A xylose absorption test for the dog. Vet. Rec 87:250
    [Google Scholar]
  9. Lee T. W., Kurtz J. B. 1977; Astroviruses detected by immunofluorescence. Lancet 2:406
    [Google Scholar]
  10. Madeley C.R., Cosgrove B. P. 1975; Viruses in infantile gastroenteritis. Lancet 2:124
    [Google Scholar]
  11. Madeley C. R., Cosgrove B. P. 1976; Caliciviruses in man. Lancet 1:199
    [Google Scholar]
  12. Madeley C. R., Cosgrove B. P., Bell E. J., Fallon R. J. 1977; Stool viruses in babies in Glasgow. 1. Hospital admissions with diarrhoea. J. Hyg., Comb 78:261
    [Google Scholar]
  13. Mavromichalis J., Evans N., McNeish A. S., Bryden A. S., Davies H. A., Flewett T. H. 1977; Intestinal damage in rotavirus and adenovirus gastroenteritis assessed by D-xylose malabsorption. Archs Dis. Child 52:589
    [Google Scholar]
  14. Mebus C. A., Stair E. L., Rhodes M. B., Twiehaus M. J. 1973; Neonatal calf diarrhea: propagation, attenuation and characteristics of a coronavirus-like agent. Am. J. vet. Res 34:145
    [Google Scholar]
  15. Morin M., Lariviere S., Lallier R. 1976; Pathological and microbiological observations made on spontaneous cases of acute neonatal calf diarrhoea. Can. J. comp. Med 40:228
    [Google Scholar]
  16. Roberts M. C. 1974; The D (+) xylose absorption test in the horse. Eq. vet. J 6:28
    [Google Scholar]
  17. Roe J. H., Rice E. W. 1948; A photometric method for the determination of free pentoses in animal tissues. J. biol. Chem 173:507
    [Google Scholar]
  18. Scherrer R., Cohen J., L’Haridon R., Feynerol C., Fayet J. C. 1976; Identification of a rotavirus associated with neonatal calf diarrhoea in France. Bull. Off. int. Epizoot 85:23
    [Google Scholar]
  19. Snodgrass D. R., Angus K. W., Gray E. W. 1977; Rotavirus infection in lambs: pathogenesis and pathology. Archs Virol 55:287
    [Google Scholar]
  20. Storz J., Bates R. C. 1973; Parvovirus infections in calves. J. Am. vet. med. Assoc 163:884
    [Google Scholar]
  21. Woode G. N. 1969; Transmissible gastroenteritis of swine. Vet. Bull 39:239
    [Google Scholar]
  22. Woode G. N., Bridger J. C. 1975; Viral enteritis of calves. Vet. Rec 96:85
    [Google Scholar]
  23. Woode G. N., Bridger J. C., Hall G. A., Dennis M. J. 1974; The isolation of a reovirus-like agent associated with diarrhoea in colostrum deprived calves in Great Britain. Res. vet. Sci 16:102
    [Google Scholar]
  24. Woode G. N., Bridger J. C., Jones J. M., Flewett T. H., Bryden A. S., Davies H. A., White G. B. B. 1976; Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice and foals. Infect. Immun 14:804
    [Google Scholar]
  25. Woode G. N., Bridger J. C., Meyling A. 1978; Significance of bovine coronavirus infection. Vet. Rec 102:15
    [Google Scholar]
  26. Woode G. N., Smith C., Dennis M. J. 1978; Intestinal damage in rotavirus infected calves assessed by D-xylose malabsorption. Vet. Rec 102:340
    [Google Scholar]
  27. Zwillenberg L. O., Burki F. 1966; On the capsid structure of some small feline and bovine RNA viruses. Arch. ges. Virusforsch 19:373
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-11-4-441
Loading
/content/journal/jmm/10.1099/00222615-11-4-441
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error