1887

Abstract

SUMMARY

Antibody responses were studied in human patients from whom leptospiral serovars—mainly or —had been isolated and identified. The antibody to the polysaccharide F4 antigen belonged exclusively to the IgM class, even as late as 10 months after infection. Human sera cross-reacted widely with F4 antigen from heterologous serovars. The antibodies involved in leptospiral agglutination were mainly IgM, but some patients also produced IgG agglutinins. The titres of IgM agglutinins were higher than those of IgG agglutinins and persisted for many months, regardless of the presence or absence of IgG agglutinins. Both types of immunoglobulin from patients with serovar infection protected hamsters against lethal infections with homologous leptospires. The hamster-protective capacity of human sera correlated well with agglutinin titres. Sera from patients infected with serovars other than protected hamsters against challenge with only if they contained agglutinins to that organism.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-11-4-387
1978-11-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/11/4/medmicro-11-4-387.html?itemId=/content/journal/jmm/10.1099/00222615-11-4-387&mimeType=html&fmt=ahah

References

  1. Adler B., Faine S. 1976; Susceptibility of mice treated with cyclophosphamide to lethal infection with Leptospira interrogans serovar pomona. Infect. Immun 14:703
    [Google Scholar]
  2. Adler B., Faine S. 1978; Serological and protective antibody responses of rabbits to leptospiral antigens. J. Med. Microbiol 11:401
    [Google Scholar]
  3. Alston J. M., Broom J. C. 1958 Leptospirosis in man and animals Edinburgh:
    [Google Scholar]
  4. Babudieri B. 1961; Laboratory diagnosis of leptospirosis. Bull. Wld Hlth Org 24:45
    [Google Scholar]
  5. Borg-Petersen C. 1953 In Symposium on the leptospiroses. Walter Reed Army Medical Center, Med. Sci. Publ No. 1, p 174
    [Google Scholar]
  6. Chang R. S., McComb D. E. 1954; Erythrocyte sensitizing substances from five strains of Leptospirae. Am. J. trop. Med. Hyg 3:481
    [Google Scholar]
  7. Christmas B. W., Bragger J. M., Till D. G. 1974; Dairyfarm fever in New Zealand: isolation of L. pomona and L. hardjo from a local outbreak. N.Z. Med. J 79:904
    [Google Scholar]
  8. Combiescu D., Sturdza N., Sefer M., Klipper A. 1960; Evolution des agglutinines dans les leptospiroses humaines. Valeur de la reaction d’agglutination-lyse dans la determination du type etiologique. Archs roum. Path. exp. Microbiol 19:201
    [Google Scholar]
  9. Cox C. D. 1955; Hemolysis of sheep erythrocytes sensitized with leptospiral extracts. Proc. Soc. exp. Biol. Med 90:610
    [Google Scholar]
  10. Cox C. D., Alexander A. D., Murphy L. C. 1957; Evaluation of the hemolytic test in the serodiagnosis of human leptospirosis. J. infect. Dis 101:210
    [Google Scholar]
  11. Crawford R. P. 1972; Molecular characteristics of antibody detected by the microscopic agglutination test in serum of guinea pigs with leptospirosis. Am. J. vet. Res 33:1507
    [Google Scholar]
  12. Faine S., Adler B., Palit A. 1974; Chemical serological and biological properties of a serotype-specific polysaccharide antigen in Leptospira. Aust. J. exp. Biol. med. Sci 52:311
    [Google Scholar]
  13. Graves S., Faine S. 1970; Antileptospiral agglutinins produced in rabbits. Bull. Wld Hlth Org 43:579
    [Google Scholar]
  14. Hartmann L., Filliti-wurmser S., Jaquot-armand Y., Mailloux M., Hurez D., Fauvert R. 1964; Nature macromoleculaire d’un anticorps de la leptospirose australis. Biochim. biophys. Acta 82:249
    [Google Scholar]
  15. Johnson R. C., Bey R. F., Auran N. E. 1975; The immunogenic properties of the outer envelope of parasitic leptospires. National Symposium on Leptospirosis, Leptospira and other Spirochaeta, Bucharest. abst
    [Google Scholar]
  16. Johnson R. C., Harris V. G. 1967; Differentiation of pathogenic and saprophytic leptospires. I. Growth at low temperatures. J. Bact 94:27
    [Google Scholar]
  17. McComb D. E., Smith D. J. W., Coffin D. L., MacReady R. A., Chang R. S. 1957; The use of erythrocyte sensitizing substance in the diagnosis of leptospiroses. I. The sensitized erythrocyte agglutination test. Am. J. trop. Med. Hyg 6:90
    [Google Scholar]
  18. Morris J. A., Hussaini S. N. 1974; Characterization of the antibodies detected by the microscopic agglutination test for bovine leptospirosis. J. Hyg., Camb 73:425
    [Google Scholar]
  19. Morsi H. M., Shibley G. P., Strother H. L. 1973; Antibody response of swine to Leptospira canicola and Leptospira icterohaemorrhagiae. Am. J. vet. Res 34:1253
    [Google Scholar]
  20. Negi S. K., Myers W. L., Segre D. 1971; Antibody response of cattle to Leptospira pomona: response as measured by hemagglutination, microscopic agglutination and hamster protection tests. Am. J. vet. Res 32:1915
    [Google Scholar]
  21. Palit A., Gulasekharam J. 1973; Genus-specific leptospiral antigen and its possible use in laboratory diagnosis. J. clin. Path 26:7
    [Google Scholar]
  22. Pike R. M. 1967; Antibody heterogeneity and serological reactions. Bact. Rev 31:157
    [Google Scholar]
  23. Pike R. M., McBrayer H. L., Schulze M. L., Chandler C. H. 1965; Chromatographic analysis and sulfhydryl sensitivity of antileptospira agglutinins in rabbit and human sera. Proc. Soc. exp. Biol. Med 120:786
    [Google Scholar]
  24. Schüffner W., Mochtar A. 1926–27; Versuche zur Aufteilung von Leptospiren-stämmen, mit ein leitenden Bemerkungen über den Verlauf von Agglutination und Lysis. Zentbl. Bakt. ParasitKde, I. Abt. Orig 101:405
    [Google Scholar]
  25. Sulzer C. R., Glosser J. W., Rogers F., Jones W. L., Frix M. 1975; Evaluation of an indirect hemagglutination test for the diagnosis of human leptospirosis. J. clin. Microbiol 2:218
    [Google Scholar]
  26. Tong M. J., Rosenberg E. B., Votteri B. A., Che C. T. 1971; Immunological response in leptospirosis. Report of three cases. Am. J. trop. Med. Hyg 20:625
    [Google Scholar]
  27. Turner L. H. 1968; Leptospirosis II. Trans. R. Soc. trop. Med. Hyg 62:880
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-11-4-387
Loading
/content/journal/jmm/10.1099/00222615-11-4-387
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error