Epsilon toxin, at relatively low concentrations, killed guinea-pig peritoneal macrophages . The cells became swollen, the nuclear and cytoplasmic membranes “blistered” and discontinuous, and the cytoplasm appeared structureless. Formalinised epsilon prototoxin was shown to bind closely to the outer surface of the cells and it is concluded that this site represents the location of the receptors for epsilon toxin.

In addition the toxin at higher concentrations killed rabbit peritoneal macrophages after increased periods of incubation, but had no demonstrable effect on other cells from guinea-pigs, rabbits, mice and sheep.


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  1. Buxton D. 1976; Use of horseradish peroxidase to study the antagonism of Clostridium welchii (Cl. perfringens) type D epsilon toxin in mice by the formalinized epsilon proto-toxin. J. comp. Path 86:67
    [Google Scholar]
  2. Buxton D. 1978a; The use of an immunoperoxidase technique to investigate by light and electron microscopy the sites of binding of Clostridium welchii type-D epsilon toxin. J. med. Microbiol 11:289
    [Google Scholar]
  3. Buxton D. 1978b; Further studies on the mode of action of Clostridium welchii type-D epsilon toxin. J. med. Microbiol. 11:293
    [Google Scholar]
  4. Douglas S. D., Zuckerman S. H., Ooka M. P. 1976; Effects of cholera exotoxin on the mitochondrial morphology of lymphoid cells and mononuclear phagocytes. Lab. Invest 35:607
    [Google Scholar]
  5. Fraser H., Hancock P. M. 1977; An investigation of the macrophage electrophoretic mobility test in the diagnosis of scrapie in sheep. J. comp. Path 87:267
    [Google Scholar]
  6. Stuart A. E. 1970 The reticulo-endothelial system Edinburgh and London:
    [Google Scholar]

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