The Use of An Immunoperoxidase Technique to Investigate by Light and Electron Microscopy the Sites of Binding of Type-D Epsilon Toxin in Mice Free

Abstract

SUMMARY

Mice were given an intravenous dose of formalinised type-D epsilon prototoxin and an immunoperoxidase technique was used to demonstrate this antigen in the tissues. The antigen was found to bind to the luminal surface of the endothelial lining of certain blood vessels, to the luminal surface of the cells lining the loops of Henlé and distal convoluted tubules in the kidney, and to the hepatic sinusoids. As it has been shown previously that formalinised epsilon prototoxin and epsilon toxin can compete for the same receptor sites it is postulated that the binding sites demonstrated represent the location of the receptors for type-D epsilon toxin.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-11-3-289
1978-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/11/3/medmicro-11-3-289.html?itemId=/content/journal/jmm/10.1099/00222615-11-3-289&mimeType=html&fmt=ahah

References

  1. Avrameas S. 1969; Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry 6:43
    [Google Scholar]
  2. Burns J. 1975; Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material. Histochemistry 43:291
    [Google Scholar]
  3. Buxton D. 1976; Use of horseradish peroxidase to study the antagonism of Clostridium welchii (Cl. perfringens) type D epsilon toxin in mice by the formalinized epsilon prototoxin. J. comp. Path 86:67
    [Google Scholar]
  4. Cruickshank R. 1965 Medical microbiology 11th ed Edinburgh and London: p 839
    [Google Scholar]
  5. Gardner D. E. 1974; Brain oedema: an experimental model. Br. J. exp. Path 55:453
    [Google Scholar]
  6. Habeeb A. F. S. A. 1969; Studies on ε-prototoxin of Clostridium perfringens type D. I. Purification methods: evidence for multiple forms of ε-prototoxin. Archs Biochem. Biophys 130:430
    [Google Scholar]
  7. Kawarai Y., Nakane P. K. 1970; Localization of tissue antigens on the ultrathin sections with peroxidase-labeled antibody method. J. Histochem. Cytochem 18:161
    [Google Scholar]
  8. Laliberte F., Sapin C., Druet P., Bariety J. 1975; Ultrastructural study by immunoperoxidase of a rat membranous glomerulonephritis. J. Ultrastruct. Res 50:150
    [Google Scholar]
  9. Morgan K. T., Kelly B. G. 1974; Ultrastructural study of brain lesions produced in mice by the administration of Clostridium welchii type D toxin. J. comp. Path 84:181
    [Google Scholar]
  10. Morgan K. T., Kelly B. G., Buxton D. 1975; Vascular leakage produced in the brains of mice by Clostridium welchii type D toxin. J. comp. Path 85:461
    [Google Scholar]
  11. Petrusz P., Di Meo P., Ordronneau P., Weaver C., Keefer D. A. 1975; Improved immunoglobulin-enzyme bridge method for light microscopic demonstration of hormone-containing cells of the rat adenohypophysis. Histochemistry 46:9
    [Google Scholar]
  12. Webb J. A., Dorling J. 1972; The use of peroxidase-labelled antiglobulin for ultrastructural localization of tissue antigens reacting with serum antibodies. J. immun. Meth 2:145
    [Google Scholar]
  13. Zacks S. I., Sheff M. F. 1968; Tetanus toxin: fine structure localization of binding sites in striated muscle. Science, N. Y 159:643
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-11-3-289
Loading
/content/journal/jmm/10.1099/00222615-11-3-289
Loading

Data & Media loading...

Most cited Most Cited RSS feed