The production of lipase and phospholipase by certain members of the Enterobacteriaceae was examined by thin-layer chromatography of resting-cell suspensions incubated with triolein or lecithin. Most strains of produced both enzymes while most strains of exhibited strong lipase but only a minor phospholipase activity. spp. (25 strains), (20 strains), (15 strains), (7 strains) and spp. (20 strains) lacked both types of enzymic activity except for the following: three strains of , two of and three of possessed slight lipase activity; about one-half of the and strains examined produced slight phospholipase activity. It is suggested that tests for lipase and phospholipase should be used in conjunction with those for DNAase production and sugar fermentation for the differentiation of and


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