1887

Abstract

SUMMARY

The value of RVA, N-l, 7H10, 7H11 and Sauton’s media for studies on mycobacterio-phage infection and lysis of mycobacteria was assessed. Experiments were made with mycobacteriophages BGI, BKI, CRI-3, G37, and LG, all of which lyse strain 607B, and with mycobacteriophage DS6A which lyses strain H37Rv. The methods involved “direct lysis”, the measurement of “routine test dilutions” and counts of plaque-forming units. It was found that N-l, 7H10 and 7H11 media gave better overall results than RVA medium for strain 607B and its phages, and that RVA medium was generally the most useful for strain H37Rv and its phage. Sauton’s medium was inadequate for all the phage-host systems employed.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-11-2-187
1971-05-01
2022-01-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/11/2/medmicro-11-2-187.html?itemId=/content/journal/jmm/10.1099/00222615-11-2-187&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 Bacteriophages New York:
    [Google Scholar]
  2. Belfanti S. 1912; Die vitale reaktion nach Gosio beim tuberkelbazillus. Z. Chemother. 1: Orig 113
    [Google Scholar]
  3. Cohn M. L., Waggoner R. S., McClatchy J. K. 1968; 7H11 Medium for cultivation of mycobacteria. Am. Rev. resp. Dis 98:295
    [Google Scholar]
  4. Corper H. H. 1915; Sodium tellurite as a rapid test for the viability of tubercle bacilli. Studies on the biochemistry and chemotherapy of tuberculosis. J. infect. Dis 16:47
    [Google Scholar]
  5. Gosio B. 1905; Indicatoren des bakterienlebens und ihre praktische bedeutung. Z. Hyg. InfektKrankh 51:65
    [Google Scholar]
  6. Middlebrook G., Cohn M. L. 1958; Bacteriology of tuberculosis. Laboratory methods. Am. J. publ. Hlth 48:844
    [Google Scholar]
  7. Redmond W. B., Ward D. M. 1966; Media and methods for phage-typing mycobacteria. Bull. Wld Hlth Org 35:563
    [Google Scholar]
  8. Šula L. 1947; The reduction of potassium tellurite by Mycobacterium tuberculosis. Am. Rev. Tuberc 56:241
    [Google Scholar]
  9. Šula L., Šulová J. 1963; The tellurite zonal phenomenon of mycobacterial lysis observed in a BCG strain exposed to mycophage My F2P/59. Am. Rev. resp. Dis 88:77
    [Google Scholar]
  10. Šula L., Šulová J. 1969; A comparative study of special agar medium (Redmond) and simple agar medium for the phage-typing of mycobacteria. Bull. Wld Hlth Org 41:17
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-11-2-187
Loading
/content/journal/jmm/10.1099/00222615-11-2-187
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error