1887

Abstract

SUMMARY

Firm agar media containing 3-6% agar-three times the concentration of agar in routine media-inhibited the swarming of 167 strains of and 14 strains incubated in air. The swarming of was also prevented by firm agar when incubation was anaerobic, provided that a negative pressure of 600-650 mm of mercury was generated in the removal of air from jars before admitting hydrogen for the palladium-catalysed removal of residua] traces of oxygen. When a negative pressure of only 300 mm of mercury was generated there was a tendency for colonies of to develop outgrowths after incubation for 2 days. Firm agar inhibited the swarming of and

As judged by a viable-count method, firm agar medium permitted the growth of maximal numbers of colonies for all organisms tested except the organisms included and

Precautions necessary for the preparation of firm agar media are defined. Colonies of a number of bacterial species on firm agar are described and illustrated. Firm agar media, being applicable to all bacteria with swarming colonies and practically non-inhibitory to other bacteria, are recommended for the isolation of bacteria from clinical specimens that may contain swarmers.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-11-2-155
1978-02-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jmm/11/2/medmicro-11-2-155.html?itemId=/content/journal/jmm/10.1099/00222615-11-2-155&mimeType=html&fmt=ahah

References

  1. Alwen J., Smith D. G. 1967; A medium to suppress the swarming of Proteus species. J. appl. Bad 30:389
    [Google Scholar]
  2. Armitage J. P., Rowbury R. J., Smith D. G. 1974; The effects of chloramphenicol, nalidixic acid and penicillin on the growth and division of swarming cells of Proteus mirabilis. J. med. Microbiol 7:459
    [Google Scholar]
  3. Armitage J. P., Rowbury R. J., Smith D. G. 1975; Indirect evidence for cell wall and membrane differences between filamentous swarming cells and short non-swarming cells of Proteus mirabilis. J. gen. Microbiol 89:199
    [Google Scholar]
  4. Barer G. 1946; The rapid detection of gelatin-liquefying organisms. Mon. Bull. Minist. Hlth 5:28
    [Google Scholar]
  5. Bisset K. A. 1973a; The motion of the swarm of Proteus mirabilis. J. med. Microbiol 6:33
    [Google Scholar]
  6. Bisset K. A. 1973b; The zonation phenomenon and structure of the swarm colony in Proteus mirabilis. J. med. Microbiol 6:429
    [Google Scholar]
  7. Bisset K. A., Douglas C. W. I. 1976; A continuous study of morphological phase in the swarm of Proteus. J. med. Microbiol 9:229
    [Google Scholar]
  8. Brogan T. D., Nettleton J., Reid C. 1971; The swarming of Proteus on semi-synthetic media. J. med. Microbiol 4:1
    [Google Scholar]
  9. Cowan S. T., Steel K. J. 1974 Manual for the identification of medical bacteria 2nd ed Cambridge:
    [Google Scholar]
  10. Crowley N., Bradley J. M., Darrell J. H. 1969 Practical bacteriology London: p 97
    [Google Scholar]
  11. Fuscoe F. J. 1973; The role of extracellular slime secretion in the swarming of Proteus. Med. Lab. Technol 30:373
    [Google Scholar]
  12. Hayward N. J. 1945; The examination of wounds for Clostridia. Proc. Ass. clin. Path 1:5
    [Google Scholar]
  13. Hayward N. J. 1947; Laboratory diagnosis of anaerobic infections. In Recent advances in clinical pathology edited by Dyke S. C. London: p 76
    [Google Scholar]
  14. Hayward N. J., Miles A. A. 1943; Inhibition of Proteus in cultures from wounds. Lancet 2:116
    [Google Scholar]
  15. Miles A. A., Misra S. S., Irwin J. O. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb 38:732
    [Google Scholar]
  16. Roux S. T., Appelbaum P. C. 1972; A phage-inhibitor for the swarming of Proteus. J. med. Microbiol 5:377
    [Google Scholar]
  17. Smith D. G. 1972; The Proteus swarming phenomenon. Sci. Prog., Lond 60:487
    [Google Scholar]
  18. Smith D. G. 1975; Inhibition of swarming in Proteus spp. by tannic acid.. appl. Bact 38:29
    [Google Scholar]
  19. Smith D. G., Alwen J. 1966; Effect of activated charcoal on the swarming of Proteus. Nature, Lond 212:941
    [Google Scholar]
  20. Stokes E. J. 1960 Clinical bacteriology 2nd ed London: p 113
    [Google Scholar]
  21. Stokes E. J. 1975; Clinical bacteriology, 4th ed.. London p 149
    [Google Scholar]
  22. Williams F. D. 1973a; Abolition of swarming of Proteus by p-nitrophenyl glycerin: general properties. Appl. Microbiol 25:745
    [Google Scholar]
  23. Williams F. D. 1973b; Abolition of swarming of Proteus by ^-nitrophenyl glycerin: application to blood agar media. Appl. Microbiol 25:751
    [Google Scholar]
  24. Williams K. 1971; Some observations on Clostridium tetani. Med. Lab. Technol 28:399
    [Google Scholar]
  25. Willis A. T., Williams K. 1970; Some cultural reactions of Clostridium tetani. J. med. Microbiol 3:291
    [Google Scholar]
  26. Willis A. T., Williams K. 1972; Prevention of swarming of Clostridium septicum. J. med. Microbiol 5:493
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-11-2-155
Loading
/content/journal/jmm/10.1099/00222615-11-2-155
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error