Detoxification of Staphylococcal Toxin by Hydrocortisone and Methylprednisolone Free

Abstract

SUMMARY

The effects of hydrocortisone (HC) and methylprednisolone (MP) on the biological activities of staphylococcol α toxin were studied. Incubation of either HC or MP at concentrations of 4-8 mg per ml with purified a toxin (690 HU per ml) reduced or eliminated haemolytic activity for rabbit red cells, intraperitoneal lethality for mice, and dermonecrotic activity for rabbits. Detoxification was related to the dose of steroid, and MP was slightly more active than HC. The mechanism of detoxification apparently involves a direct molecular interaction between a toxin and steroid, and pretreatment of animals with HC or MP did not enhance their resistance to the subsequent effects of toxin.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-11-1-67
1978-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/11/1/medmicro-11-1-67.html?itemId=/content/journal/jmm/10.1099/00222615-11-1-67&mimeType=html&fmt=ahah

References

  1. Agarwal K. N., Garby L. 1964; Inhibition by corticosteroids of red cell lysis in vitro. Acta endocrinol Suppl 93:3
    [Google Scholar]
  2. Arbuthnott J. P., Freer J. H., Billcliffe B. 1973; Lipid-induced polymerization of staphylococcal α-toxin. J. gen. Microbiol 75:309
    [Google Scholar]
  3. Bernheimer A. W., Schwartz L. L. 1963; Isolation and composition of staphylococcal α-toxin. J. gen. Microbiol 30:455
    [Google Scholar]
  4. Cassidy P., Harshman S. 1976; Studies on the binding of staphylococcal 125I-labelled α-toxin to rabbit erythrocytes. Biochemistry 15:2348
    [Google Scholar]
  5. Fischer H. 1961–62; Considerations on the improved preservation of erythrocytes. Folia haemat., Lpz 78:624
    [Google Scholar]
  6. Greene A. E., Ambrus J. L., Gershenfeld L. 1935; Effect of cortisone and desoxy-corticosterone on infection with tetanus spores and upon the toxicity of tetanus toxin. Antibiotics Chemother 3:1121
    [Google Scholar]
  7. Jennings J. F., Taylor G. 1964; Effect of hydrocortisone hemisuccinate on immune lysis of sheep erythrocytes. Nature, Lond 203:661
    [Google Scholar]
  8. Kapral F. A. 1974; Staphylococcus aureus: some host-parasite interactions. Ann. N. Y. Acad. Sci 236:267
    [Google Scholar]
  9. Klainer A. S., Madoff M. A., Cooper L. Z., Weinstein L. 1964; Staphylococcal alpha-hemolysin: detection on the erythrocyte membrane by immunofluorescence. Science, N. Y 145:714
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Randall R. J., Farr A. L. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  11. Orsi N., Poggiolini D., Terzani G. 1962; A study of the action of various steroids on staphylococcal alpha hemolysin. Riv. Biol 55:375
    [Google Scholar]
  12. Rahal J. J. 1972; Comparative effects of purified staphylococcal alpha and delta toxins on mitochondrial metabolism. J. infect. Dis 126:96
    [Google Scholar]
  13. Seneca H., Kupyn O., Johnson A. 1957; Effect of metisteroids on Clostridium tetani, Clostridium welchii and Micrococcus pyogenes var. aureus toxins. Antibiotics Chemother 7:155
    [Google Scholar]
  14. Werner A. S., Ribble J. C. 1967; Staphylococcal infection in chick embryos: the role of staphylococcal toxins in death of chick embryos. J. infect. Dis 117:91
    [Google Scholar]
  15. Zwemer R. L., Jungeblut C. W. 1935; Effect of various corticoadrenal extracts on diphtheria toxin in vivo and in vitro. Proc. Soc. exp. Biol. Med 32:1583
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-11-1-67
Loading
/content/journal/jmm/10.1099/00222615-11-1-67
Loading

Data & Media loading...

Most cited Most Cited RSS feed