1887

Abstract

SUMMARY

Polyagglutinable (PA) strains of are agglutinated by more than one of the antisera prepared against antigenically unrelated O serogroups. They form . 4% of strains—other than isolates from cystic-fibrosis units—submitted to us for typing. We were able to allocate 80% of PA strains to a single O serogroup by agglutination with typing sera that had been absorbed with the polyagglutinable strain SMC-247, or by co-agglutination tests with protein A-containing staphylococci coated with immunoglobulin from unabsorbed sera. Similar results were obtained by precipitation tests with crude bacterial extracts and unabsorbed sera, but these tests were less sensitive and less specific. Evidence is presented that PA antigen is a heat-stable cell constituent distinct from the O antigen. In rabbit antisera, anti-PA antibody is exclusively of the IgM class, but O antibody of both IgM and IgG classes is present.

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1978-02-01
2022-01-17
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References

  1. Asheshov E. H. 1974; An assessment of the methods used for typing strains of Pseudomonas aeruginosa. In Proceedings of the sixth national congress of bacteriology edited by A. Arseni Athens. p 9
    [Google Scholar]
  2. Bergan T. 1975; Epidemiological typing of Pseudomonas aeruginosa. In Resistance of Pseudomonas aeruginosa edited by Brown M. R. W. New York: p 189
    [Google Scholar]
  3. Boivin A., Mesrobeanu L. 1937; Sur l’antigene O; endotoxine des pyocyaniques. C. r. Seanc. Soc. Biol 125:273
    [Google Scholar]
  4. Chester I. R., Meadow P. M. 1975; Heterogeneity of the lipopolysaccharide from Pseudomonas aeruginosa. Eur. J. Biochem 58:273
    [Google Scholar]
  5. Chester I. R., Meadow P. M., Pitt T. L. 1973; The relationship between the O-antigenic lipopolysaccharides and serological specificity in strains of Pseudomonas aeruginosa of different O-serotypes. J. gen. Microbiol 78:305
    [Google Scholar]
  6. Christie R. 1948; Observations on the biochemical and serological characteristics of Pseudomonas pyocyanea. Aust. J. exp. Biol. med. Sci 26:425
    [Google Scholar]
  7. Duncan N. H., Hinton N. A., Penner J. L., Duncan I. B. R. 1976; Preparation of typing antisera specific for O antigens of Pseudomonas aeruginosa. J. clin. Microbiol. 4:124
    [Google Scholar]
  8. Habs I. 1957; Untersuchungen fiber die O-Antigene von Pseudomonas aeruginosa. Z. Hyg. InfektKrankh 144:218
    [Google Scholar]
  9. Homma J. Y., Kim K. S., Yamada H., Ito M., Shionoya H., Kawabe Y. 1970; Serological typing of Pseudomonas aeruginosa and its cross infection. Jap. J. exp. Med 40:347
    [Google Scholar]
  10. Homma J. Y., Shionoya H., Yamada H., Kawabe Y. 1971; Production of antibody against Pseudomonas aeruginosa and its serological typing. Jap. J. exp. Med 41:89
    [Google Scholar]
  11. Köhler W. 1957; Zur Serologie der Pseudomonas aeruginosa. Z. ImmunForsch. exp. Ther 114:282
    [Google Scholar]
  12. Kronvall G. 1973; A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci. J. med. Microbiol 6:187
    [Google Scholar]
  13. Lányi B. 1966–67; Serological properties of Pseudomonas aeruginosa 1. Group-specific somatic antigens. Acta microbiol. hung 13:295
    [Google Scholar]
  14. Martin D. R. 1971; Variation in Pseudomonas aeruginosa. Ph.D. Thesis University of London;
    [Google Scholar]
  15. Meitert T. 1964; Contribution á l’étude de la structure antigénique des B. pyocyaniques (Pseudomonas aeruginosa). II. Individualisation des groupes serologiques au moyen des antigénes “O”. Archs roum. Path. exp. Microbiol 23:679
    [Google Scholar]
  16. Mikkelsen O. S. 1970; Serotyping of Pseudomonas aeruginosa. II. Results of an O group classification. Acta path, microbiol. scand 78B:163
    [Google Scholar]
  17. Ouchterlony O. 1949; Antigen-antibody reactions in gels. Acta path, microbiol. scand 26:507
    [Google Scholar]
  18. Sandvik O. 1960; Serological comparison between strains of Pseudomonas aeruginosa from human and animal sources. Acta path, microbiol. scand 48:56
    [Google Scholar]
  19. Verder E., Evans J. 1961; A proposed antigenic schema for the identification of strains of Pseudomonas aeruginosa. J. infect. Dis 109:183
    [Google Scholar]
  20. Véron M. 1961; Sur l’agglutination de Pseudomonas aeruginosa: subdivision des groupes antigeniques 0.2 et 0.5. Annls Inst. Pasteur, Paris 101:456
    [Google Scholar]
  21. Wilson G. S., Miles A. A. 1975 In Topley and Wilson’s Principles of bacteriology, virology and immunity 6th ed., edited by Wilson G. S., Miles A. A. London: vol 2 p 1381
    [Google Scholar]
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