Concentrated non-toxic preparations of colicine V were obtained by filtering centrifugates of soft-agar cultures of a Col V+ K12 strain of E. coli through Millipore filters in which the colicine V was retained. These preparations, given subcutaneously, favourably influenced the course of disease in mice infected intraperitoneally with a pathogenic strain of E. coli that was very sensitive to colicine V in vitro. A beneficial effect was noted even when treatment was delayed until the mice were visibly ill.
BraudeA. I.,
SiemienskiJ. S.1965; The influence of bacteriocins on resistance to infection by Gram-negative bacteria. I. The effect of colicin on bactericidal power of blood. J. clin. Invest 44:849
BraudeA. I.,
SiemienskiJ. S.1968; The influence of bacteriocins on resistance to infection by Gram-negative bacteria. II. Colicin action, transfer of colicinogeny, and transfer of antibiotic resistance in urinary infections. J. clin. Invest 47:1763
SmithH.,
Williams and HugginsM. B.1976; Further observations on the association of the colicine V plasmid of Escherichia coli with pathogenicity and with survival in the alimentary tract. J. gen. Microbiol 92:335