1887

Abstract

SUMMARY

Culture filtrates and total water-soluble extracts (TWSE), including ultra-sonication products of were primarily fractionated by electrophoresis in polyacrylamide gels. Other separations included chromato-graphy with Con A-Separose and human immunoglobulins from sera that contained precipitins to farmer’s lung hay (FLH) antigen coupled to Sepharose 4B by the cyanogen-bromide method. Fractions were tested for their effects on the lung by the intranasal inoculation of non-immunised mice and of mice immunised with TWSE in Freund’s complete adjuvant to give precipitin-negative and precipitin-positive groups. Particulate antigens of did not induce respiratory disease in non-immunised mice, but TWSE produced negligible, sporadic lesions. Precipitin-positive mice developed symptoms of extrinsic allergic alveolitis when challenged with particulate antigens consisting of living or dead spores and mycelium of or with TWSE. Elements of both Type III (Arthus) and Type IV (cell-mediated) hypersensitivities were identified histologically. The allergenicity of spores and mycelium was almost nullified by de-fatting, and activity was retained solely in a serologically inert ethanol-ether extract. Fractionation of soluble antigens produced immuno-logically-acti ve extracts, only some of which induced clinical disease. Alveolitis-inducing glycoprotein antigens obtained from Con A-Sepharose by desorption with α-methyl-D-glucopyranoside precipitated in the C region on immuno-electrophoresis. A disease-provoking group of antigens precipitating in the A region was isolated by electrophoresis in agar after primary fractionation in polyacrylamide gel. Antigens desorbed both by 3.0 acetic acid and 6.0 guanidine-HCl from immunoadsorbent columns also precipitated in the A region, but only those desorbed by guanidine-HCl induced alveolitis.

Most fractions inducing disease in precipitin-positive mice also did so in precipitin-negative, immunised animals. The formation of large hyper-sensitivity granulomata, indicative of the occurrence of a Type IV cell-mediated response, followed the introduction of bentonite particles coated with reactive fractions into the lung. Mean diameters of the granulomata did not correlate directly with the degree of alveolitis induced by the fractions. Skin reactions were bimodal and showed early Type III and late Type IV histology.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-10-3-331
1977-08-01
2022-07-03
Loading full text...

Full text loading...

/deliver/fulltext/jmm/10/3/medmicro-10-3-331.html?itemId=/content/journal/jmm/10.1099/00222615-10-3-331&mimeType=html&fmt=ahah

References

  1. Aspberg K., Porath J. 1970; Group-specific adsorption of glycoproteins. Acta chem. scand 24:1839
    [Google Scholar]
  2. Barrowcliff D. F., Arblaster P. G. 1968; Farmer’s lung: a study of an early acute fatal case. Thorax 23:490
    [Google Scholar]
  3. Boros D. L., Warren K. S. 1970; Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs. J. exp. Med 132:488
    [Google Scholar]
  4. Boros D. L., Warren K. S. 1973; The bentonite granuloma. Characterization of a model system for infectious and foreign body granulomatous inflammation using soluble mycobacterial, histoplasma and schistosoma antigens. Immunology 24:511
    [Google Scholar]
  5. Boros D. L., Warren K. S. 1974; Models of granulomatous inflammation. Ann. N.Y. Acad. Sci 221:331
    [Google Scholar]
  6. Cuatrecasas P., Anfinsen C. B. 1971; Affinity chromatography. Meth. Enzym 22:345
    [Google Scholar]
  7. Edwards J. H. 1971; The production of farmer’s lung antigens. Med. Lab. Technol 28:172
    [Google Scholar]
  8. Edwards J. H. 1972; The isolation of antigens associated with farmer’s lung. Clin. exp. Immun 11:341
    [Google Scholar]
  9. Edwards J. H. 1974; Experimental immunopathology with specific antigen fractions. In Aspergillosis and farmer’s lung in man and animal edited by de Haller R., Suter F. Bern, Stuttgart and Vienna: p 269
    [Google Scholar]
  10. Edwards J. H., Baker J. T., Davies B. H. 1974; Precipitin test negative farmer’s lungactivation of the alternative pathway of complement by mouldy hay dusts. Clin. Allergy 4:379
    [Google Scholar]
  11. Fletcher S. M., Rondle C. J. M., Murray I. G. 1970; The extracellular antigens of Micropolyspora faeni: their significance in farmer’s lung disease. J. Hyg., Camb 68:401
    [Google Scholar]
  12. Gell P. G. H., Coombs R. R. A. 1968 Clinical aspects of immunology 2nd ed Oxford:
    [Google Scholar]
  13. Grant I. W. B., Blyth W., Wardrop V. E., Gordon R. M., Pearson J. C. G., Mair A. 1972; Prevalence of farmer’s lung in Scotland: a pilot survey. Br. med. J 1:530
    [Google Scholar]
  14. Heide K., Schwick H. G. 1973 In Handbook of experimental immunology 2nd ed., edited by Weir D. M. Oxford: vol 1: p 6.2
    [Google Scholar]
  15. Herbert W. J. 1973 In Handbook of experimental immunology 2nd ed., edited by Weir D. M. Oxford: vol 3: p A2.12
    [Google Scholar]
  16. Herbert D., Phipps P. J., Strange R. E. 1971 In Methods in microbiology vol 5B: edited by Norris J. R., Ribbons D. W. London and New York: pp 284–285
    [Google Scholar]
  17. Hollingdale M. R. 1974; Antibody responses in patients with farmer’s lung disease to antigens from Micropolyspora faeni. J. Hyg., Camb 72:79
    [Google Scholar]
  18. Hollingdale M. R. 1975; Isolation of lipopolysaccharide from the walls of Micropolyspora faeni: chemical composition and serological reactivity. J. gen. Microbiol 86:250
    [Google Scholar]
  19. Hollingdale M. R., Murray I. G. 1974; Antibody response in farmer’s lung disease. In Aspergillosis and farmer’s lung in man and animal edited by de Haller R., Suter F. Bern, Stuttgart and Vienna: p 280
    [Google Scholar]
  20. Jonasson O., Becker E. L. 1966; Release of kallikrein from guinea pig lung during anaphylaxis. J. exp. Med 123:509
    [Google Scholar]
  21. Kennedy J. F., Rosevear A. 1973; An assessment of the fractionation of carbohydrates on Concanavalin A-Sepharose 4B by affinity chromatography. J. chem. Soc Perkin Trans; I:2041
    [Google Scholar]
  22. Molenaar J. L., Muller M., Pondman K. W. 1973; A new preparative method for isolation of human C3 with affinity chromatography. J. Immun 110:1570
    [Google Scholar]
  23. Moore S., Stein W. H. 1948; Photometric ninhydrin method for use in the chromatography of amino acids. J. biol. Chem 176:367
    [Google Scholar]
  24. Pepys J., Jenkins P. A. 1965; Precipitin (FLH) test in farmer’s lung. Thorax 20:21
    [Google Scholar]
  25. Pepys J., Jenkins P. A., Festenstein G. N., Gregory P. H., Lacey M. E., Skinner F. A. 1963; Farmer’s lung. Thermophilic actinomycetes as a source of “ farmer’s lung hay ” antigen. Lancet 2:607
    [Google Scholar]
  26. Roberts R. C. 1974; Fractionation and chemical characterization studies on Micropolyspora faeni antigens. Ann. N. Y. Acad. Sci 221:199
    [Google Scholar]
  27. Salvaggio J., Phanuphak P., Stanford R., Bice D., Claman H. 1975; Experimental production of granulomatous pneumonitis. J. Allergy clin. Immun 56:364
    [Google Scholar]
  28. Scheidegger J. J. 1955; Une micro-methode de l’immuno-electrophorese. Int. Archs Allergy 7:103
    [Google Scholar]
  29. Seal R. M. E., Hapke E. J., Thomas G. O., Meek J. C., Hayes M. 1968; The pathology of the acute and chronic stages of farmer’s lung. Thorax 23:469
    [Google Scholar]
  30. Wilkie B., Pauli B., Gygax M. 1973; Hypersensitivity pneumonitis; experimental production in guinea pigs with antigens of Micropolyspora faeni. Pathologia Microbiol 39,:393
    [Google Scholar]
  31. Zaidi S. H., Dogra R. K. S., Shanker R., Chandra S. V. 1971; Experimental farmer’s lung in guinea-pigs. J. Path 105:41
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-10-3-331
Loading
/content/journal/jmm/10.1099/00222615-10-3-331
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error