Three procedures for the anaerobic culture of clinical specimens are compared: (i) a conventional bench routine, (ii) a system whereby primary anaerobic plates were incubated for 48 h before examination, and (iii) an anaerobic chamber.
It was shown that the anaerobic chamber was of no advantage over bench regimens when primary anaerobic plates were incubated for 48 h before being examined.
ArankiA.,
SyedS. A.,
KenneyE. B.,
FreterR.1969; Isolation of anaerobic bacteria from human gingiva and mouse caecum by means of a simplified glove box procedure. Appl. Microbiol 17:568
KilligoreG. E.,
StarrS. E.,
Del BeneV. E.,
WhaleyD. N.,
DowellV. R.Jr1973; Comparison of three anaerobic systems for the isolation of anaerobic bacteria from clinical specimens. Am. J. clin. Path 59:552
McMinnM. T.,
CrawfordJ. J.1970; Recovery of anaerobic microorganisms from clinical specimens in prereduced media versus recovery by routine clinical laboratory methods. Appl. Microbiol 19:207
SpauldingE. H.,
VargoV.,
MichaelsonT. C.,
SwensonR. M.1974; A comparison of two procedures for isolating anaerobic bacteria from clinical specimens. In Anaerobic bacteria: role in disease edited by
BalowsA.,
DeHaanR. M.,
DowellV. R.,
GuzeL. B.
Springfield, Illinois: p 37
SpearsR.,
FreterR.1967; Improved isolation of anaerobic bacteria from the mouse caecum by maintaining continuous strict anaerobiosis. Proc. Soc. exp. Biol. Med 124:903
SutterV. L.,
VargoV.,
FinegoldS. M.1975; Wadsworth Anaerobic Bacteriology Manual. 2nd ed Department of Continuing Education in Health Sciences, University Extension Los Angeles:
VargoV.,
MichaelsonT. C.,
SpauldingE. H.,
VitagllanoR.,
SwensonR. M.,
ForschE.1971; Comparison of pre-reduced anaerobic methods and Gas-Pak for isolating anaerobic bacteria. Bad. Proc p 109
WattB.,
ColleeJ. G.1974; Practical approaches to the isolation and identification of clinically important non-sporing anaerobes. In Infection with non-sporing anaerobic bacteria edited by
PhillipsI.,
Sussman.
London: p 7
WattB.,
ColleeJ. G.,
BrownR.1974; The isolation of strict anaerobes: the use of an anaerobic cabinet compared with a conventional procedure. J. med. Microbiol 7:315