1887

Abstract

SUMMARY

Strains of that are lysed by typing phages 94 or 96, or by both phages, are usually resistant to lysis by other basic-set typing phages. They are, however, sensitive to several experimental phages and show a number of different lytic patterns when tested against these phages. These differences in susceptibility are due, in part, to immunity imposed by temperate phages carried by the different strains. Resistance to lysis by other basic-set phages was not due to prophage immunity, but to at least one restriction and modification system in such strains. Restrictionless mutants were isolated from one strain in several experiments. These showed an increased sensitivity to many basic-set phages. However, all of these mutants retained the ability to modify the phages to the characteristic “94, 96” specificity.

Strains in the 94, 96 complex showed a remarkable homogeneity in biological traits. The majority were non-pigmented, and produced lipase, fibrinolysin, and haemolysins and enterotoxin B. This homogeneity may well be a reflexion of the restriction and modification systems in these strains, that prevent the acquisition of genetic material from strains outside the complex. A new lytic group V is proposed for members of the 94, 96 complex.

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1977-05-01
2021-10-22
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References

  1. Blair J. E., Williams R. E. O. 1961; Phage typing of staphylococci. Bull. Wld Hlth Org 24:771
    [Google Scholar]
  2. Blouse L. E., Stringfield W. B., Marraro R. V., Dupuy H. J. 1973; Activity and characteristics of a new staphylococcal phage 94. Proc. Soc. exp. Biol. Med 142:572
    [Google Scholar]
  3. Boven C. P. A., Stobberingh E. E. van, Verhoef J., Winkler K. C. 1974; Restriction and modification of phages in staphylococcal phage typing. Ann. N. Y. Acad. Sci 236:376
    [Google Scholar]
  4. Elek S. D., Levy E. 1950; Distribution of haemolysins in pathogenic and nonpathogenic staphylococci. J. Path. Bad 62:541
    [Google Scholar]
  5. Lack C. 1956; Biological characteristics of staphylococci recovered from pathologic materials. Ann. N. Y. Acad. Sci 65:103
    [Google Scholar]
  6. Novick R. P. 1963; Analysis by transduction of mutations affecting penicillinase formation in Staphylococcus aureus. J. gen. Microbiol 33:121
    [Google Scholar]
  7. Ralston D. J., Baer B. S. 1964a; A new property of phage group II Staphylococcus aureus strains: host restriction of phage K14. J. gen. Microbiol 36:1
    [Google Scholar]
  8. Ralston D. J., Baer B. S. 1964b; Propagation of staphylococcal typing phages on a common host, Staphylococcus aureus Kl, and host-controlled changes in their lytic range. J. gen. Microbiol 36:17
    [Google Scholar]
  9. Ralston D. J., Baer B. S. 1964c; Host-controlled changes in staphylococcal phage 3C affecting its broad group typing pattern. J. gen. Microbiol 36:25
    [Google Scholar]
  10. Sierra G. 1957; A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek 23:15
    [Google Scholar]
  11. Šimkovičová M., Gilbert R. J. 1971; Serological detection of enterotoxin from food-poisoning strains of Staphylococcus aureus. J. med. Microbiol 4:19
    [Google Scholar]
  12. Subcommittee on Phage-typing of Staphylococci 1975; Report (1970-74) to the International Committee on Systematic Bacteriology. Int. J. syst. Bact 25:241
    [Google Scholar]
  13. Willis A. T., Smith J. A., O’Connor J. J. 1960; Properties of some epidemic strains of Staphylococcus aureus. J. Path. Bact 92:345
    [Google Scholar]
  14. Wood W. B. 1966; Host specificity of DNA produced by Escherichia coli bacterial mutations affecting the restriction and modification of DNA. J. molec. Biol 16:118
    [Google Scholar]
  15. Yoshimori R., Roulland-Dussoix D., Boyer H. W. 1972; R factor-controlled restriction and modification of deoxyribonucleic acid: restriction mutants. J. Bact 112:1275
    [Google Scholar]
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