1887

Abstract

SUMMARY

When used to determine immunopurified antibacterial globulins in saline-phosphate buffer, the detection sensitivities of direct competitive and indirect immunoradiometric assays were respectively 25 and 2 ng. Normal human and rabbit sera interfered in both types of assay, markedly decreasing sensitivity and precision. Various pre-treatments of sera and modifications of reaction conditions substantially decreased interference in the competitive assay without affecting the activity of added antibody, but they had only marginal effects in the indirect assay. When serum samples taken from rabbits after vaccination with the Live Vaccine strain of were tested with the two assays and agglutination tests, newly synthesised circulating antibody was usually detected earlier by the competitive assay than by the other methods.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-10-2-151
1977-05-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jmm/10/2/medmicro-10-2-151.html?itemId=/content/journal/jmm/10.1099/00222615-10-2-151&mimeType=html&fmt=ahah

References

  1. Boyden S. V. 1966; Natural antibodies and the immune response. Adv. Immun 5:1
    [Google Scholar]
  2. Eigelsbach H. T., Downs C. M. 1961; Prophylactic effectiveness of live and killed tularemia vaccines. I. Production of vaccine and evaluation in the white mouse and guinea pig. J. Immun. 87:415
    [Google Scholar]
  3. Haimovich J., Tarrab R., Sulica A., Sela M. 1970; Antibodies of different specifici ties in normal rabbit sera. J. Immun. 104:1033
    [Google Scholar]
  4. Hambleton P., Evans C. G. T., Hood A. M., Strange R. E. 1974; Vaccine potencies of the live vaccine strain of Francisella tularensis and isolated bacterial components. Br. J. exp. Path 55:363
    [Google Scholar]
  5. Hunter W. M., Greenwood F. C. 1962; Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, Lond 194:495
    [Google Scholar]
  6. Inman J. K. 1974; Multispecificity of the antibody combining region and antibody diversity. In The immune system: genes, receptors, signals, edited by E. E. Sercarz, A. R. Williamson and C. F. Fox. London and New York p 37
    [Google Scholar]
  7. Jerne N. K. 1955; The natural-selection theory of antibody formation. Proc. natl Acad. Sci., USA 41:849
    [Google Scholar]
  8. Jerne N. K. 1960; Immunological speculations. A. Rev. Microbiol 14:341
    [Google Scholar]
  9. Nassau E., Parsons E. R., Johnson G. D. 1975; Detection of antibodies to Mycobacterium tuberculosis by solid phase radioimmunoassay. J. immunol. Methods 6:261
    [Google Scholar]
  10. Nielsen K. H., Parratt D., White R. G. 1973; Quantitation of antibody to particulate antigens using a radiolabelled anti-immunoglobulin reagent: application to estimation of antibody in farmer’s lung syndrome. J. immunol. Methods 3:301
    [Google Scholar]
  11. Restall S. W. F., Thomas G., Pennant J. H. 1973; A radioimmunoassay technique for the titration of bacterial antisera. Microbiological Research Establishment Report. no. 75
    [Google Scholar]
  12. Rosenthal J. D., Hayashi K., Notkins A. L. 1972; Rapid micro-radioimmunoassay for the measurement of antiviral antibody. J. Immun. 109:171
    [Google Scholar]
  13. Scharer J. M., Klein F., Lincoln R. E. 1968; Growth and metabolism of the Live Vaccine strain of Pasteurella tularensis. Appl. Microbiol 16:855
    [Google Scholar]
  14. Smith K. O., Gehle W. D., McCracken A. W. 1974; Radioimmunoassay techniques for detecting naturally occurring viral antibody in human sera. J. immunol. Methods 5:337
    [Google Scholar]
  15. Strange R. E., Hambleton P. 1976; An indirect immunoradiometric assay of microbes. J. gen. Microbiol 93:401
    [Google Scholar]
  16. Strange R. E., Martin K. L. 1972; Rapid assays for the detection and determination of sparse populations of bacteria and bacteriophage T7 with radioactively labelled homologous antibodies. J. gen. Microbiol 72:127
    [Google Scholar]
  17. Strange R. E., Powell E. O., Pearce T. W. 1971; The rapid detection and determination of sparse bacterial populations with radioactively labelled homologous antibodies. J. gen. Microbiol 67:349
    [Google Scholar]
  18. Tigertt W. D. 1962; Soviet viable Pasteurella tularensis vaccines: a review of selected articles. Bact. Rev. 26:354
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-10-2-151
Loading
/content/journal/jmm/10.1099/00222615-10-2-151
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error