1887

Abstract

SUMMARY

Sodium aurothiomalate (ATM), gold keratinate and five different tetra-cyclines were investigated for activity against strain ATCC 14124 and strain JB, both and in rodents with arthritis caused by these mycoplasmas. , ATM had only slight activity against and , while gold keratinate was virtually inactive against . In contrast, the tetracyclines were highly active against both mycoplasmas. The tetracyclines and the gold salts were both predominantly mycoplasmastatic.

In both rats and mice, parenteral administration of ATM, begun shortly before or after infection of the rodents with mycoplasmas, prevented the development of arthritis. ATM or gold keratinate, given subcutaneously to mice already arthritic from infection with , reduced the severity of the arthritis, even although gold keratinate was inactive against this mycoplasma . Moreover, direct testing of serum, collected from mice treated with gold keratinate, failed to demonstrate antimycoplasmal activity . These results suggest that the action of gold-containing drugs in mycoplasmal arthritis is due to biological properties of gold other than antimycoplasmal activity.

Tetracyclines were also found to be effective in preventing arthritis in rats and mice when given subcutaneously. With high doses, subcutaneous, but not oral, therapy significantly reduced the severity of established arthritis in mice infected with . The blood levels achieved with the different tetracyclines, when related to their therapeutic activity, indicated that good antimycoplasmal activity and adequate absorption from the gut were not the only properties needed for optimal effectiveness. The results are discussed in relation to treatment of rheumatoid patients with tetracycline HC1.

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1977-02-01
2022-01-17
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References

  1. Barden J. A., Tully J. G. 1969; Experimental arthritis in mice with Mycoplasma pulmonis. J. Bact 100:5
    [Google Scholar]
  2. Brown T. McP., Wichelhausen R. H., Robinson L. B., Merchant W. R. 1949; The in-vivo action of aureomycin on pleuropneumonia-like organisms associated with various rheumatic diseases. J. Lab. clin. Med 34:1404
    [Google Scholar]
  3. Brown T. McP., Bush S. W., Felts W. R. 1959; “Rheumatoid diseases and gout. In Long-term illness, management of the chronically ill patient”. edited by Wohl M. G. Philadelphia and London: p 93
    [Google Scholar]
  4. Cole B. C., Ward J. R., Jones R. S., Cahill J. F. 1971; Chronic, proliferative arthritis of mice induced by Mycoplasma arthritidis. I. Induction of disease and histopathological characteristics. Infect. Immun 4:344
    [Google Scholar]
  5. Davidson M., Thomas L. 1966; Effect of gold on Mycoplasma. In Antimicrobial agents and chemotherapy 1966 edited by Hobby G. L. Ann Arbor, Mich: p 312
    [Google Scholar]
  6. Findlay G. M., Mackenzie R. D., MacCallum F. O. 1940; Chemotherapeutic experiments on pleuropneumonia-like organisms in rodents. Br. J. exp. Path 21:13
    [Google Scholar]
  7. Findlay G. M., Mackenzie R. D., MacCallum F. O., Klieneberger E. 1939; The aetiology of polyarthritis in the rat. Lancet 2:7
    [Google Scholar]
  8. Ford D. K. 1972; Inhibition of growth of T-strain mycoplasmas by hydroxamic acids and by aurothiomalate. Antimicrob. Agents Chemother 2:340
    [Google Scholar]
  9. Hannan P. C. T., Hughes B. O. 1971; Reproducible polyarthritis in rats caused by Mycoplasma arthritidis. Ann. rheum. Dis 30:316
    [Google Scholar]
  10. Jasmin G. 1957a; Experimental polyarthritis in rats injected with tumour exudate. Ann. rheum. Dis 16:365
    [Google Scholar]
  11. Jasmin G. 1957b; Prevention of experimental polyarthritis with sodium aurothiomalate. J. Pharmac. exp. Ther 120:349
    [Google Scholar]
  12. Jessop J. D., Yernon-Roberts B., Harris J. 1973; Effects of gold salts and prednisolone on inflammatory cells. I. Phagocytic activity of macrophages and polymorphs in inflammatory exudates studied by a “ skin window ” technique in rheumatoid and control patients. Ann. rheum. Dis 32:294
    [Google Scholar]
  13. Kuzell W. C., Gardner G. M. 1950; Salicyclazosulfapyridine (Salazopyrin or Azopyrin) in rheumatoid arthritis and experimental polyarthritis. Calif. Med 73:476
    [Google Scholar]
  14. Lawrence J. S. 1961; Studies with radioactive gold. Ann. rheum. Dis 20:341
    [Google Scholar]
  15. Lorber A., Gifford D. B., Chandor S. B., Carroll P. E. 1974; Evidence of immunosuppression by gold salts in rheumatoid arthritis. Clin. Pharmac. Ther 15:211
    [Google Scholar]
  16. Measel J. W. JR 1975; Effect of gold on the immune response of mice. Infect Immun 11:350
    [Google Scholar]
  17. Miles A. A., Misra S. S., Irwin J. O. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb 38:732
    [Google Scholar]
  18. Newnham A. G., Chu H. P. 1965; An in vitro comparison of the effect of some antibacterial, antifungal and antiprotozoal agents on various strains of Mycoplasma (pleuropneumonia-like organisms: P.P.L.O.). J. Hyg., Camb 63:1
    [Google Scholar]
  19. Parkes M. W., Wrigley F. 1951; Arthritis in rats produced by pleuropneumonia-like organisms. Ann. rheum. Dis 10:177
    [Google Scholar]
  20. Powell H. M., Jamieson W. A., Rice R. M. 1946; Effectiveness of streptomycin in arthritis of rats. Proc. Soc. exp. Biol. Med 62:8
    [Google Scholar]
  21. Powell H. M., Rice R. M. 1944; Ineffective penicillin chemotherapy of arthritic mice infected with pleuropneumonia-like organisms. J. Lab. clin. Med 29:372
    [Google Scholar]
  22. Preston W. S. 1942; Arthritis in rats caused by pleuropneumonia-like microorganisms and the relationship of similar organisms to human rheumatism. J. infect. Dis 70:180
    [Google Scholar]
  23. Preston W. S., Block W. D., Freyberg R. H. 1942; Chemotherapy of chronic progressive arthritis of mice. I. Role of sulfur in gold-containing compounds. Proc. Soc. exp. Biol. Med 50:253
    [Google Scholar]
  24. Robinson L. B., Wichelhausen R. H., Brown T. McP. 1952; Sensitivity studies on human pleuropneumonia-like organisms. J. Lab. clin. Med 39:290
    [Google Scholar]
  25. Sabin A. B. 1939; Experimental proliferative arthritis in mice produced by filterable, pleuropneumonia-like microorganisms. Science, N. Y 89:228
    [Google Scholar]
  26. Sabin A. B., Warren J. 1940; The curative effect of certain gold compounds on experimental proliferative, chronic arthritis in mice. J. Bact 40:823
    [Google Scholar]
  27. Sanchez I. 1968; As tetracyclinas no tratamento da artrite rheumatoide e dorengas rheumaticas inflamatorias. Braz.-Med 82:22
    [Google Scholar]
  28. Schultz D. R., Volanakis J. E., Arnold P. I., Gottlieb N. L., Sakai K., Stroud R. M. 1974; Inactivation of Cl in rheumatoid synovial fluid, purified Cl and Cl esterase, by gold compounds. Clin. exp. Immun 17:395
    [Google Scholar]
  29. Schutze E. 1968; Mykoplasma-Modell-Infektonen bei kleinen Laboratoriumstieren. Zentbl Bakt. ParasitKde, Abt. 1 Orig 208:301
    [Google Scholar]
  30. Siegel S. 1956; The Mann-Whitney U Test.. In Nonparametric statistics for the behavioural sciences edited by Harlow H. F. New York, Toronto and London: pp 116–127
    [Google Scholar]
  31. Skinner M., Cathcart E. S., Mills J. A., Pinals R. S. 1971; Tetracycline in the treatment of rheumatoid arthritis. A double blind controlled study. Arthritis Rheum 14:727
    [Google Scholar]
  32. Stewart S. M., Burnet M. E., Young J. E. 1969; In-vitro sensitivity of strains of mycoplasmas from human sources to antibiotics and to sodium aurothiomalate and tylosin tartrate. J. med. Microbiol 2:287
    [Google Scholar]
  33. Stewart S. M., Duthie J. R., MacKay J. M. K., Marmion B. P., Alexander W. R. M. 1974; Mycoplasmas and rheumatoid arthritis. Ann. rheum. Dis 33:346
    [Google Scholar]
  34. Taylor-Robinson D. 1967; Mycoplasmas of various hosts and their antibiotic sensitivities. Post-grad. med. J 43: Suppl. [March] 100
    [Google Scholar]
  35. Tripi H. B., Kuzell W. C. 1947; Production of experimental polyarthritis by pleuropneumonia-like (L4) organisms in rats and preliminary results on protective effects of a gold product. Stanford med. Bull 5:98
    [Google Scholar]
  36. Vernon-Roberts B., Jessop J. D., Dore J. 1973; Effects of gold salts and prednisolone on inflammatory cells. II. Suppression of inflammation and phagocytosis in the rat. Ann. rheum. Dis 32:301
    [Google Scholar]
  37. Weissman G. 1966; Lysosomes and joint disease. Arthritis Rheum 9:834
    [Google Scholar]
  38. Woglom W. H., Warren J. 1938; A pyogenic filterable agent in the albino rat. J. exp. Med 68:513
    [Google Scholar]
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