1887

Abstract

SUMMARY

A simple, reliable and highly discriminating scheme for the bacteriocine typing of has been developed. Strains are typed on MacConkey’s agar according to their ability to produce a proticine active against one of 14 indicator strains having a single and specific proticine sensitivity and also according to their sensitivity to the different proticines of 13 proticine-producing strains. This new scheme of combined production and sensitivity typing was formulated after 250 strains of from clinical material had been examined for the production of proticines active against the 24 indicator strains of Cradock-Watson’s proticine typing scheme and for proticine activity and sensitivity towards each other. Three new types of proticinogenic strains were discovered and defined. Strains producing proticines of types 1, 2 and 3 were isolated frequently. These common proticines could be subtyped by their different actions on newly characterised indicator strains.

By means of this production/sensitivity (P/S) typing scheme, 250 strains were differentiated into 90 distinct types, whereas typing by sensitivity alone distinguished only 40 types and typing by production alone distinguished only 20 types (including subtypes).

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-10-1-7
1977-02-01
2024-11-09
Loading full text...

Full text loading...

/deliver/fulltext/jmm/10/1/medmicro-10-1-7.html?itemId=/content/journal/jmm/10.1099/00222615-10-1-7&mimeType=html&fmt=ahah

References

  1. Adler J. L., Burke J. P., Martin D. F., Finland M. 1971; Proteus infection in a general hospital. 1. Biochemical characteristics and antibiotic susceptibility of the organisms with special reference to proticine typing and Dienes phenomena. Annls int. Med 75:517
    [Google Scholar]
  2. Al-Jumaili I. J. 1975a; Bacteriocine typing of Proteus. J. clin. Path 28:784
    [Google Scholar]
  3. Al-Jumaili I. J. 1975b; An evaluation of two methods of bacteriocine typing of organisms of the genus Proteus. J. clin. Path 28:788
    [Google Scholar]
  4. Belyavin G., Miles E. M., Miles A. A. 1951; The serology of fifty strains of Proteus vulgaris. J. gen. Microbiol 5:178
    [Google Scholar]
  5. Burke J. P., Ingall D., Klein J. O., Gezon H. M., Finland M. 1971; Proteus mirabilis infections in a hospital nursery traced to a human carrier. New Engl. J. Med 284:115
    [Google Scholar]
  6. Cradock-Watson J. E. 1965; The production of bacteriocines by Proteus species. Zentbl. Bakt. ParasitKde., I. Abt. Orig 196:385
    [Google Scholar]
  7. De Louvois J. 1969; Serotyping and the Dienes reaction on Proteus mirabilis from hospital infections. J. clin. Path 22:263
    [Google Scholar]
  8. Finland M. 1960; Treatment of pneumonia and other serious infections. New Engl. J. Med 263:207
    [Google Scholar]
  9. France D. R., Markham N. P. 1968; Epidemiological aspects of Proteus infections with particular reference to phage typing. J. clin. Path 21:97
    [Google Scholar]
  10. George R. H. 1974; Comparison of different media for bacteriocine typing of Proteus mirabilis. J. clin. Path 28:25
    [Google Scholar]
  11. Huang C. T. 1966; Multitest media for rapid identification of proteus species with notes on biochemical reactions of strains from urine and pus. J. clin. Path 19:438
    [Google Scholar]
  12. Kashbur I. M., George R. H., Ayliffe G. A. J. 1974; Resistotyping of Proteus mirabilis and a comparison with other methods of typing. J. clin. Path 27:572
    [Google Scholar]
  13. Kippax P. W. 1957; A study of proteus infections in a male urological ward. J. clin. Path 10:211
    [Google Scholar]
  14. Krikler M. S. 1953; The serology of Proteus vulgaris. Ph.D. Thesis University of London:
  15. Lewis J., Fekety F. R. 1969; Proteus bacteremia. Johns Hopkins med. J 124:151
    [Google Scholar]
  16. Shortland-Webb W. R. 1968; Proteus and coliform meningoencephalitis in neonates. J. clin. Path 21:422
    [Google Scholar]
  17. Skirrow M. B. 1969; The Dienes (mutual inhibition) test in the investigation of Proteus infections. J. med. Microbiol 2:471
    [Google Scholar]
  18. Story P. 1954; Proteus infections in hospitals. J. Path. Bad 68:55
    [Google Scholar]
  19. Tracy O., Thompson E. J. 1972; An evaluation of three methods of typing organisms of the genus Proteus. J. clin. Path 25:69
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-10-1-7
Loading
/content/journal/jmm/10.1099/00222615-10-1-7
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error