1887

Abstract

Summary

Typing of meningococci with a panel of serotype and subtype specific monoclonal antibodies (MAbs) was compared in co-agglutination, dot-blotting and ELISA tests. Twenty reference strains, 50 case isolates and 133 throat isolates from healthy carriers were studied. The typing results with dot-blotting and ELISA were identical, whereas co-agglutination gave different results for three case and 24 carrier strains. The distribution of serotypes and subtypes among the strains is reported. The combination of the subtypes P1.1 and P1.15 in a serotype 15 patient strain was observed. With one case strain and 15 carrier strains, neither serotype nor subtype could be determined. Non-typable and non-subtypable isolates were further characterised by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Co-agglutination is useful for typing small numbers of strains with a few MAbs, but less suitable for large-scale typing than the other two methods. Dot-blotting needs less expensive equipment, smaller volumes of antibodies and fewer manipulations than ELISA.

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1990-03-01
2024-03-29
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References

  1. Branham S E. Serological relationships among meningococci. BacteriolRev 1953; 17:175–188
    [Google Scholar]
  2. Craven D E, Frasch C E, Robbins J B, Feldman H A. Serogroup identification of Neisseria meningitidis: comparison of an antiserum agar method with bacterial slide agglutination. J Clin Microbiol 1978; 7:410–414
    [Google Scholar]
  3. Gotschlich E C. The status of vaccines to meningococcal and gonococcal disease. Ann Inst Pasteur Microbiol 1985; 136B:341–355
    [Google Scholar]
  4. Frasch C E, Zollinger W D, Poolman J T. Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev Infect Dis 1985; 7:504–510
    [Google Scholar]
  5. Sugasawara R J. The use of monoclonal antibodies for detecting and serotyping Neisseria meningitidis. In Macario A J L, de Macario E C. Monoclonal antibodies against bacteria 11: Orlando: Academic Press; 198661–79
    [Google Scholar]
  6. Frnrholm L O, Bøvre K, Holten E, Zollinger W D. Serotyping of meningococci by coagglutination with monoclonal antibodies. NIPH Ann 1983; 6:125–138
    [Google Scholar]
  7. Zollinger W D, Moran E E, Connelly H, Mandrell R E, Brandt B. Monoclonal antibodies to serotype 2 and serotype 15 outer membrane proteins of Neisseria meningitidis and their use in serotyping. Infect Immun 1984; 46:260–266
    [Google Scholar]
  8. de Marie S, Hoeijemakers J H J, Poolman J T, Zanen H C. Filter radioimmunoassay a method for large-scale serotyping of Neisseria meningitidis. J Clin Microbiol 1984; 20:255–258
    [Google Scholar]
  9. Abdillahi H, Poolman J T. Whole-cell ELISA for typing Neisseria meningitidis with monoclonal antibodies. FEMSMicrobiolLett 1987; 48:367–371
    [Google Scholar]
  10. Bol P, Spanjaard L, Hopman C, Arends A, Kersten F, Zanen H C. Epidemiology of meningococcal (sub)types in the Netherlands. Poolman J T. Gonococci and meningococci Dordrecht: Kluwer Academic Publishers; 198827–32
    [Google Scholar]
  11. Sandven P, Solberg O, Ødegaard K, Myhre G. Improved medium for the transportation of gonococcal specimens. Acta Pathol Microbiol Immunol Scand Scand[B] 1982; 90:73–77
    [Google Scholar]
  12. Ødegaard K, Solberg O, Lind J, Myhre G, Nyland B. Lincomycin in selective medium for the isolation of Neisseria gonorrhoeae. Acta Pathol Microbwl Scand [B] 1975; 83:301–304
    [Google Scholar]
  13. Wedege E, Frnrholm L O. Human antibody response to a group B serotype 2a meningococcal vaccine determined by immunoblotting. Infect Immun 1986; 51:571–578
    [Google Scholar]
  14. Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  15. Wedege E, Michaelsen T E. Human immunoglobulin G subclass immune responses toouter membrane antigens in meningococcal group B vaccine. J Clin Microbiol 1987; 25:1349–1353
    [Google Scholar]
  16. Käyhty H, Sivonen A, Poolmann J, Peltola H, Eskola J, MäkeläP H. Types and subtypes of group B meningococci isolated in Finland from 1976 through April 1986. Poolman J T. Gonococci and meningococci Dordrecht: Kluwer Academic Publishers; 1988119–124
    [Google Scholar]
  17. Abdillahi H. Monoclonal antibodies and Neisseria meningitidis. Typing and subtyping for epidemiological surveillance and vaccine developmentAcademic dissertation Utrecht: 1988160–163
    [Google Scholar]
  18. Abdillahi H, Crowe B A, Achtman M, Poolman J T. Two monoclonal antibodies specific for serotype 4 antigen of Neisseria meningitidis. Eur J Clin Microbiol Infect Dis 1988; 7:293–296
    [Google Scholar]
  19. Caugant D A, Bønrvre K, Gaustad P, Bryn K, Holten E, Hnriby E A, Frnrholm L O. Multilocus genotypes determined by enzyme electrophresis of Neisseria meningitidis isolated from patients with systemic disease and from healthy carriers. J Gen Microbwl 1986; 132:641–652
    [Google Scholar]
  20. Caugant D A, Kristiansen B E, FrøholmL O, Bøsvre K, Selander R K. Clonal diversity of Neisseria meningitidis from a population of asymptomatic carriers. Infect Immun 1988; 56:2060–2068
    [Google Scholar]
  21. Kristiansen B E, Lind K W, Mevold K. et al. Meningococcal phenotypic and genotypic characteristics and human antibody levels. J Clin Microbwl 1988; 26:1988–1992
    [Google Scholar]
  22. Abdillahi H, Poolman J T. Neisseria meningitidis group B serosubtyping using monoclonal antibodies in wholecell ELISA. Microb Pathog 1988; 4:27–32
    [Google Scholar]
  23. Poolman J T, Hopman C T P, Zanen H C. Immunochemical characterization of Neisseria meningitidis serotype antigens by immunodiffusion and SDS-polyacrylamide gel electrophoresis immunoperoxidase techniques and the distribution of serotypes among cases and carriers. J Gen Microbwl 1980; 116:465–473
    [Google Scholar]
  24. Frøholm L O, Holten E, Høiby E A, Caugant D A, Zollinger W D. Monitoring of systemic meningococcal isolates from Norway. In Poolman J T. Gonococci and meningococci Dordrecht: Kluwer Academic Publishers; 1988105–108
    [Google Scholar]
  25. Abdillahi H, Poolman J T. Definition of meningococca class 1 OMP subtyping antigens by monoclonal antibodies. FEMS MicrobiolImmunol 1988; 47:139–144
    [Google Scholar]
  26. Brodeur B R, Larose Y, Tsang P, Hamel J, Ashton F, Ryan A. Protection against infection with Neisseria meningitidis group B serotype 2b by passive immunization with serotype-specific monoclonal antibody. Infect Immun 1985; 50:510–516
    [Google Scholar]
  27. Ashton F E, Ryan J A, Diena B B, Zollinger W D. Monoclonal antibody analysis of meningococcal disease strains isolated in Canada. In Poolman J T. Gonococci and meningococci Dordrecht: Kluwer Academic Publishers; 198815–19
    [Google Scholar]
  28. Tsai C M, Frasch C E, Mocca L F. Five structural classes of major outer membrane proteins in Neisseria meningitidis. J Bacteriol 1981; 146:69–78
    [Google Scholar]
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