@article{mbs:/content/journal/jmm/10.1099/0022-1317-51-5-417, author = "CHAE, GUE-TAE and KIM, MIN-JOO and KANG, TAE-JIN and LEE, SEONG-BEOM and SHIN, HANG-KYE and KIM, JONG-PILL and KO, YOUNG-HOON and KIM, SUNG-HWA and KIM, NAN-HEE", title = "DNA-PCR and RT-PCR for the 18-kDa gene of Mycobacterium leprae to assess the efficacy of multi-drug therapy for leprosy", journal= "Journal of Medical Microbiology", year = "2002", volume = "51", number = "5", pages = "417-422", doi = "https://doi.org/10.1099/0022-1317-51-5-417", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/0022-1317-51-5-417", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "DNA-PCR and reverse transcription (RT)-PCR for the 18-kDa protein of Mycobacterium leprae were used to examine the efficacy of multi-drug therapy (MDT) in leprosy. MDT was administered for 0–24 months. Fourteen (63.6%) of 22 patients showed positive PCR results after treatment for 12 months and the positive results decreased to 30% after 24 months of MDT. These results did not correlate with the bacterial index (BI) or the IgM antibody titre for the phenolic glycolipid (PGL)-1. One-dimensional densitometric analysis of agarose gels from PCR from the longitudinal study showed a gradual reduction of the 360-bp band after 12–24 months of MDT. RT-PCR for mRNA of the 18-kDa protein successfully tracked bacterial RNA changes in the biopsies and confirmed a decrease in the RNA of M. leprae in patients after MDT for 12 months. Thus, DNA- and RT-PCR for the 18-kDa protein of M. leprae are effective in assessing the efficacy of MDT for leprosy.", }