1887

Abstract

is again the subject of attention on account of the current increase in the world-wide incidence of cholera. In this study, 200 clinical isolates of serotypes O1 and non-O1, non-O139, were collected from different provinces in Iran. The isolates were subjected to biochemical analysis, antibiogram, PCR of toxin genes, plasmid profile, ribotyping and pulsed-field gel electrophoresis (PFGE). The analysis of plasmid content showed that 33–96% of isolated from different provinces carry a large plasmid. PCR analysis of O1 showed that the genes encoding cholera toxin (), toxin co-regulated pilus (), accessory cholera enterotoxin () and zonula occludens toxin () were present in 55–97% of isolates in different provinces. Restriction fragment length polymorphism (RFLP) of I-digested DNA probed with five oligonucleotides revealed three different ribotype patterns in isolates of O1. The ribotype pattern B21 of O1 El Tor was found to be the predominant pattern in the isolates studied. non-O1, non-O139 isolates showed a single ribotype pattern. PFGE analysis also showed 10 different patterns amongst the isolates, 9 of which were in O1. Overall, the analysis of polymorphism of ribotypes and PFGE patterns of the isolates showed that the provinces in Iran were affected by a limited number of clones of O1 and non-O1, non-O139 strains.

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/content/journal/jmm/10.1099/0022-1317-51-5-392
2002-05-01
2019-10-20
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/0022-1317-51-5-392
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