Microcirculation plays a crucial role in mucosal physiological function as well as repair of gastric mucosal damage. Endothelial cell damage is known to disturb microcirculation and suppress angiogenesis. Therefore, the direct effect of on endothelial cells was investigated with water extract. The effect of water extract on cell proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) was evaluated. The ratio of BrdU-positive HUVECs in both /-positive and -negative water extract-treated groups was significantly lower at 24 h than that in the control group, but water extract did not affect the proliferation of these endothelial cells. Apoptosis was induced by water extracts after incubation for 24 h in a /-independent manner. In the mitochondrial permeability transition assay, tetramethylrhodamine methyl ester was accumulated in mitochondria of HUVECs. Western blot analysis showed no difference in the level of total p53 protein in water extract-treated and non-treated cells, but the level of phosphorylated p53 protein was increased in the treated cells at 15 and 60 min after addition of the extract. Reverse transcription (RT)-PCR products for p21 and Bax were elevated in the water extract-treated cells. p21 levels began to increase 0.5–1 h after addition of the extract, whereas Bax increased in the period 0.5–2 h. induced a disturbance of cell proliferation and apoptosis in the vascular endothelial cells which may contribute to gastric mucosal injury and to delayed healing of gastric lesions.


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