1887

Abstract

An experimental infection in miniature pigs was developed and investigated. Eighteen miniature pigs were inoculated with an strain that has high virulence in mice at . 5×10cfu. infection in miniature pigs was achieved by the administration of agar 1% in brucella broth with fetal bovine serum 10% just before inoculation. The bacterial colonisation and distribution were analysed by mapping of viable cell counts in the stomach in pigs of three different ages. The mapping assay was achieved on post-infection day 3 for the 5-day-old and 2-week-old pigs, and between days 41 and 43 for 3-month-old pigs. The highest cell counts were observed in 5-day-old pigs, which averaged 4.9×105mucfu/g of mucosa (n=4). The bacteria were colonised mainly in the cardiac and fundus gland region in the 5-day-old and 2-week-old pigs, whereas the colonisation sites did not depend on the region in the 3-month-old pigs. Biopsy assay of the antral mucosa of a 3-month-old pig after infection showed that this infection persisted for >22 months. Serum antibody against was detected in the infected pigs but not in the uninfected animal. Immunostaining demonstrated the presence of bacteria on the epithelial surface of the infected pigs. A microscopic finding common to all the infected pigs, focal gastritis with infiltration of lymphocytes detected on the lesser curvature of the stomach, resembled the microscopic appearance in -infected human patients. These results suggest that miniature pigs might be a suitable model for studying infection.

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/content/journal/jmm/10.1099/0022-1317-51-3-238
2002-03-01
2019-11-22
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/0022-1317-51-3-238
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