1887

Abstract

During 1996 and 1997 an epidemic wave of meningococcal disease took place in Spain. Initial studies described the antigenic expression of the epidemic strain as C:2b:P1.2,5 and proposed that it was a variant of the previously identified Spanish C:2b:non-subtypable epidemic strain. To clarify this hypothesis, 1036 C:2b:P1.2(5) and 76 C:2b:NST isolates obtained during 1992–1999 were analysed by pulsed-field gel electrophoresis. The majority of the C:2b:P1.2,5 and C:2b:P1.2 isolates showed one of two very closely related profiles. During the epidemic period, 80% of the C:2b:NST strains showed these two pulsotypes. However, before the epidemic wave, most of these C:2b:NST strains (60%) showed a profile that was found infrequently among C:2b:P1.2,5 and C:2b:P1.2 isolates. A similar evolution was observed in C:2b:P1.5 isolates. Thirty-four C:2b:P1.2(5) and 10 C:2b:NST isolates, exhibiting representative pulsotypes, were subjected to multi-locus sequence typing. Isolates belonging to both A4 and ET-37 lineages were identified. These data point to the possibility that the A4 cluster has displaced the ET-37 complex among serogroup C meningococci in Spain.

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2002-12-01
2024-04-19
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References

  1. Schwartz B, Moore PS, Broome CV. Global epidemiology of meningococcal disease. Clin Microbiol Rev 1989; 2 Suppl:118–124
    [Google Scholar]
  2. Peltola H. Meningococcal disease: still with us. Rev Infect Dis 1983; 5:71–91 [CrossRef]
    [Google Scholar]
  3. Caugant DA. Population genetics and molecular epidemiology of Neisseria meningitidis . APMIS 1998; 106:505–525 [CrossRef]
    [Google Scholar]
  4. Caugant DA, Bovre K, Gaustad P. et al. Multilocus genotypes determined by enzyme electrophoresis of Neisseria meningitidis isolated from patients with systemic disease and from healthy carriers. J Gen Microbiol 1986; 132:641–652
    [Google Scholar]
  5. Wang J, Caugant DA, Morelli G, Koumare B, Achtman M. Antigenic and epidemiological properties of the ET-37 complex of Neisseria meningitidis . J Infect Dis 1993; 167:1320–1329 [CrossRef]
    [Google Scholar]
  6. Berrón S, Sáez-Nieto JA, Vázquez JA. Aplicación de marcadores epidemiológicos en cepas de Neisseria meningitidis con sensibilidad disminuida a penicilina aisladas en España (1985–1990). Rev Esp Quimioter 1992; 5:35–41
    [Google Scholar]
  7. Sáez-Nieto JA, García Barreno B, López Galíndez C, Casal J. Meningitis meningocócica en España (1978–1980) II.Serotipos y patrones electroforéticos en gel de poliacrilamida. Rev Sanid Hig Publica 1981; 55:1295–1308
    [Google Scholar]
  8. Berrón S, Vázquez JA. Increase in moderate penicillin resistance and serogroup C in meningococcal strains isolated in Spain.Is there any relationship?. Clin Infect Dis 1994; 18:161–165 [CrossRef]
    [Google Scholar]
  9. Vázquez JA, De La Fuente L, Berrón S. Infección meningocócica.Informe del Laboratorio de Referencia de Meningococos. Bol Epidem Sem 1995; 4:37–40
    [Google Scholar]
  10. Berrón S, De La Fuente L, Martín E, Vázquez JA. Increasing incidence of meningococcal disease in Spain associated with a new variant of serogroup C. Eur J Clin Microbiol Infect Dis 1998; 17:85–89 [CrossRef]
    [Google Scholar]
  11. Ashton FE, Ryan JA, Borczyck AL, Caugant DA, Mancino L, Huang D. Emergence of a virulent clone of Neisseria meningitidis serotype 2a that is associated with meningococcal group C disease in Canada. J Clin Microbiol 1991; 29:2489–2493
    [Google Scholar]
  12. Krizova P, Musilek M. Changing epidemiology of meningococcal invasive disease in the Czech Republic caused by a new clone Neisseria meningitidis C: 2a:P1.2 (P1.5), ET 15/37. Centr Eur J Pub Health 1995; 3:189–194
    [Google Scholar]
  13. Maiden MCJ, Bygraves JA, Feil E. et al. Multilocus sequence typing: a portable approach to the identification of clones within population of pathogenic microorganisms. Proc Natl Acad Sci USA 1998; 95:3140–3145 [CrossRef]
    [Google Scholar]
  14. Vázquez JA, Marcos C, Berrón S. Sero/subtyping of Neisseria meningitidis isolated from patients in Spain. Epidemiol Infect 1994; 113:267–274 [CrossRef]
    [Google Scholar]
  15. Tenover FC, Arbeit RD, Goering R. et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33:2233–2239
    [Google Scholar]
  16. Jacobs D. SAS/GRAPH software and numerical taxonomy. Proceedings of the 15th Annual Users Group International Conference Carey, NC, SAS Institute: 19901413–1418
    [Google Scholar]
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