1887

Abstract

The hypothesis that isolate (CR1) from an HIV-infected individual induced apoptosis of macrophages was examined by optical microscopy, binding of annexin V-FITC and analyses of DNA degradation (TUNEL tests and agarose gel electrophoresis). Resident murine peritoneal macrophages co-incubated for 5–15 min with CR1 bound annexin V, whereas macrophages incubated with either heat-inactivated strain CR1, 577 (isolated from a patient with mucocutaneous candidiasis) or FCF14 (a mutant that did not produce proteases and phospholipases) did not bind annexin for up to 2 h of observation. However, macrophages exposed to CR1 did not present the pattern of DNA degradation typical of apoptosis. Macrophages became increasingly permeable to propidium iodide from 30 min to 2 h after their exposure to CR1. Most of the phagocytosed CR1 yeast cells switched to germ-tubes inside the macrophages after incubation for 1–2 h. These results show that macrophages exposed to CR1 presented early signs of apoptosis but progressed to necrosis, and suggest that strains that readily switch to germ-tubes inside those apoptotic cells might have a competitive advantage because released germ-tubes resist further attack by macrophages.

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2002-11-01
2019-11-17
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References

  1. Zychlinsky A, Prevost MC, Sansonetti PJ. Shigella flexneri induces apoptosis in infected macrophages. Nature 1992;; 358: 167–169.[CrossRef]
    [Google Scholar]
  2. Khelef N, Zychlinky A, Guiso N. Bordetella pertussis induces apoptosis in macrophages: role of adenylate cyclase-hemolysin. Infect Immun 1993;; 61: 4064–4071.
    [Google Scholar]
  3. Baran J, Guzik K, Hryniewicz W, Ernst M, Flad HD, Pryjma J. Apoptosis of monocytes and prolonged survival of granulocytes as a result of phagocytosis of bacteria. Infect Immun 1996;; 64: 4242–4248.
    [Google Scholar]
  4. Monack DM, Raupach B, Hromockyj AE, Falkow S. Salmonella typhimurium invasion induces apoptosis in infected macrophages. Proc Natl Acad Sci USA 1996;; 93: 9833–9838.[CrossRef]
    [Google Scholar]
  5. Ruckdeschel K, Roggenkamp A, Lafont V, Mangeat P, Heesemann J, Rouot B. Interaction of Yersinia enterocolitica with macrophages leads to macrophage cell death through apoptosis. Infect Immun 1997;; 65: 4813–4821.
    [Google Scholar]
  6. Rodrigues VS, Vidotto MC, Felipe I, Santos DS, Gaziri LCJ. Apoptosis of murine peritoneal macrophages induced by an avian pathogenic strain of Escherichia coli. FEMS Microbiol Lett 1999;; 179: 73–78.[CrossRef]
    [Google Scholar]
  7. Jendrossek V, Grassmé H, Mueller I, Lang F, Gulbins E. Pseudomonas aeruginosa-induced apoptosis involves mitochondria and stress-activated protein kinases. Infect Immun 2001;; 69: 2675–2683.[CrossRef]
    [Google Scholar]
  8. Zychlinsky A, Kenny B, Ménard R, Prévost MC, Holland IB, Sansonetti PJ. Ipa B mediates macrophage apoptosis induced by Shigella flexneri. Mol Microbiol 1994;; 11: 619–627.[CrossRef]
    [Google Scholar]
  9. Chen LM, Kaniga K, Galán JE. Salmonella spp. are cytotoxic for cultured macrophages. Mol Microbiol 1996;; 21: 1101–1115.[CrossRef]
    [Google Scholar]
  10. Chen Y, Smith MR, Thirumalai K, Zychlinsky A. A bacterial invasin induces macrophage apoptosis by binding directly to ICE. EMBO J 1996;; 15: 3853–3860.
    [Google Scholar]
  11. Jacotot E, Ravagnan L, Loeffler M.et al. The HIV-1 viral protein R induces apoptosis via a direct effect on the mitochondrial permeability transition pore. J Exp Med 2000;; 191: 33–46.[CrossRef]
    [Google Scholar]
  12. Miura T, Nishikawa S, Sasaki S.et al. Role of endogenous cytokines in liver apoptosis of mice in lethal Listeria monocytogenes infection. FEMS Immunol Med Microbiol 2000;; 28: 335–341.[CrossRef]
    [Google Scholar]
  13. Perfettini JL, Darville T, Gachelin G.et al. Effect of Chlamydia trachomatis infection and subsequent tumor necrosis factor alpha secretion on apoptosis in the murine genital tract. Infect Immun 2000;; 68: 2237–2244.[CrossRef]
    [Google Scholar]
  14. Rotstein D, Parodo J, Taneja R, Marshall JC. Phagocytosis of Candida albicans induces apoptosis of human neutrophils. Shock 2000;; 14: 278–283.[CrossRef]
    [Google Scholar]
  15. Heidenreich S, Otte B, Lang D, Schmidt M. Infection by Candida albicans inhibits apoptosis of human monocytes and monocytic U937 cells. J Leukoc Biol 1996;; 60: 737–743.
    [Google Scholar]
  16. Sweeney JF, Nguyen PK, Omann GM, Hinshaw DB. Autocrine-paracrine modulation of polymorphonuclear leukocyte survival after exposure to Candida albicans. Shock 1998;; 9: 146–152.[CrossRef]
    [Google Scholar]
  17. Pacheco-Soares C, Gaziri LCJ, Loyola W, Felipe I. Phagocytosis of enteropathogenic Escherichia coli and Candida albicans by lectin-like receptors. Braz J Med Biol Res 1992;; 25: 1015–1024.
    [Google Scholar]
  18. Gaziri G, Gaziri LCJ, Kikuchi R, Scanavacca J, Felipe I. Phagocytosis of Candida albicans by concanavalin-A activated peritoneal phagocytes. Med Mycol 1999;; 37: 195–200.
    [Google Scholar]
  19. Moresco TR, Gaziri LCJ, Yasumoto Y, Felipe I. Phagocytic and candidacidal activities of macrophages from lactating and adult mice pretreated with concanavalin-A. Med Mycol (in press).
  20. Davis LG, Kuehl WM, Battey JF. Basic methods in molecular biology. Norwalk, CT, Appleton and Lange. 1994:; 16–21.
  21. Sweet SP, Cookson S, Challacombe SJ. Candida albicans isolates from HIV-infected and AIDS patients exhibit enhanced adherence to epithelial cells. J Med Microbiol 1995;; 43: 452–457.[CrossRef]
    [Google Scholar]
  22. de Bernardis F, Mondello F, Scaravelli G.et al. High aspartyl proteinase production and vaginitis in human immunodeficiency virus-infected women. J Clin Microbiol 1999;; 37: 1376–1380.
    [Google Scholar]
  23. Vargas K, Messer AS, Pfaller M.et al. Elevated phenotypic switching and drug resistance of Candida albicans from human immunodeficiency virus-positive individuals prior to first thrush episode. J Clin Microbiol 2000;; 38: 3595–3607.
    [Google Scholar]
  24. Daugas E, Nochy D, Ravagnan L.et al. Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis. FEBS Lett 2000;; 476: 118–123.[CrossRef]
    [Google Scholar]
  25. Joza N, Susin SA, Daugas E.et al. Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death. Nature 2001;; 410: 549–554.[CrossRef]
    [Google Scholar]
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