1887

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Volume 51, Issue 11

Early membrane exposure of phosphatidylserine followed by late necrosis in murine macrophages induced by from an HIV-infected individual Free

Abstract

The hypothesis that isolate (CR1) from an HIV-infected individual induced apoptosis of macrophages was examined by optical microscopy, binding of annexin V-FITC and analyses of DNA degradation (TUNEL tests and agarose gel electrophoresis). Resident murine peritoneal macrophages co-incubated for 5–15 min with CR1 bound annexin V, whereas macrophages incubated with either heat-inactivated strain CR1, 577 (isolated from a patient with mucocutaneous candidiasis) or FCF14 (a mutant that did not produce proteases and phospholipases) did not bind annexin for up to 2 h of observation. However, macrophages exposed to CR1 did not present the pattern of DNA degradation typical of apoptosis. Macrophages became increasingly permeable to propidium iodide from 30 min to 2 h after their exposure to CR1. Most of the phagocytosed CR1 yeast cells switched to germ-tubes inside the macrophages after incubation for 1–2 h. These results show that macrophages exposed to CR1 presented early signs of apoptosis but progressed to necrosis, and suggest that strains that readily switch to germ-tubes inside those apoptotic cells might have a competitive advantage because released germ-tubes resist further attack by macrophages.

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© 2002 Society for General Microbiology
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2002-11-01
2024-04-25
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Early membrane exposure of phosphatidylserine followed by late necrosis in murine macrophages induced by Candida albicans from an HIV-infected individual
J. Med. Microbiol. 51, 929 (2002); https://doi.org/10.1099/0022-1317-51-11-929
/content/journal/jmm/10.1099/0022-1317-51-11-929
/content/journal/jmm/10.1099/0022-1317-51-11-929
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