1887

Abstract

In controlling the spread of tuberculosis, early detection of disease caused by organisms of the complex (MTBC) is vital. The BD ProbeTec ET system provides a method for the direct detection of MTBC by strand displacement amplification. Two hundred and five respiratory samples from patients with a high probability of tuberculosis were assessed by ProbeTec and by microscopy and culture for mycobacteria. ProbeTec positive results were obtained with 101 of 109 samples from which MTBC organisms were isolated. ProbeTec correctly signalled 78 of 81 samples that gave growths of mycobacteria other than tubercle bacilli (MOTT) as negative. Three samples gave false-positive results, corrected on repeat testing. Positive and negative predictive values (PPV, NPV) were 0.97 and 0.90 and the system showed a sensitivity and specificity of 92.7% and 96.0%, respectively. These values rose to PPV 0.97, NPV 0.96, sensitivity 97.1% and specificity 96.0% when data from the small number of gastric lavage samples tested were removed from the analysis. The BD ProbeTec ET system offers a robust and reliable molecular biological approach to the detection of MTBC organisms in respiratory samples in a semi-automated format.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/0022-1317-51-10-895
2002-10-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/51/10/895.html?itemId=/content/journal/jmm/10.1099/0022-1317-51-10-895&mimeType=html&fmt=ahah

References

  1. World Health Organization Global tuberculosis control. World Health Organization 2000; document no. WHO/CDS/TB/2000.275
    [Google Scholar]
  2. Rose AMC, Watson JM, Graham C. et al. Tuberculosis at the end of the 20th century in England and Wales: results of a national survey in 1998. Thorax 2001; 56:173–179 [CrossRef]
    [Google Scholar]
  3. Kearns AM, Freeman R, Steward M, Magee JG. A rapid polymerase chain reaction technique for detecting M. tuberculosis in a variety of clinical specimens. J Clin Pathol 1998; 51:922–924 [CrossRef]
    [Google Scholar]
  4. French GL, Chan CY, Cheung SW, Oo KT. Diagnosis of pulmonary tuberculosis by detection of tuberculostearic acid in sputum by using gas chromatography-mass spectrometry with selected ion monitoring. J Infect Dis 1987; 156:356–362 [CrossRef]
    [Google Scholar]
  5. Gennaro ML. Immunologic diagnosis of tuberculosis. Clin Infect Dis 2000; 30 Suppl 3:S243–S246 [CrossRef]
    [Google Scholar]
  6. Salfinger M. Diagnosis of tuberculosis and other diseases caused by mycobacteria. Infection 1997; 25:60–62 [CrossRef]
    [Google Scholar]
  7. Freeman R, Magee JG, Watt B, Rayner AB. Being positive about the smear. Thorax 2001; 56:417–418 [CrossRef]
    [Google Scholar]
  8. Behr MA, Warren SA, Salamon H. et al. Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli. Lancet 1999; 353:444–449 [CrossRef]
    [Google Scholar]
  9. Lamden K, Watson JM, Knerer G, Ryan MJ, Jenkins PA. Opportunist mycobacteria in England and Wales: 1982–1994. Commun Dis Rep CDR Review 1996; 11:R147–R151
    [Google Scholar]
  10. Noordhoek GT, Kolk AHJ, Bjune G. et al. Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis : a blind comparison study among seven laboratories. J Clin Microbiol 1994; 32:277–284
    [Google Scholar]
  11. Hawkey PM. The role of polymerase chain reaction in the diagnosis of mycobacterial infections. Rev Med Microbiol 1994; 5:21–32
    [Google Scholar]
  12. Walker GT, Fraiser MS, Schram JL. et al. Strand displacement amplification – an isothermal, in vitro DNA amplification technique. Nucleic Acids Res 1992; 20:1691–1696 [CrossRef]
    [Google Scholar]
  13. Little MC, Andrews J, Moore R. et al. Strand displacement amplification and homogeneous real-time detection incorporated in a second-generation DNA probe system, BDProbeTecET. Clin Chem 1999; 45:777–784
    [Google Scholar]
  14. Freeman R, Magee J, Barrett A. Identifying sputum specimens of high priority for examination by enhanced mycobacterial detection, identification, and susceptibility systems (EMDISS) to promote the rapid diagnosis of infectious pulmonary tuberculosis. J Clin Pathol 2001; 54:613–616 [CrossRef]
    [Google Scholar]
  15. Anon Update: Nucleic acid amplification tests for tuberculosis. MMWR Morb Mortal Wkly Rep 2000; 49:593–594
    [Google Scholar]
  16. Down JA, O'Connell MA, Dey MS. et al. Detection of Mycobacterium tuberculosis in respiratory specimens by strand displacement amplification of DNA. J Clin Microbiol 1996; 34:860–865
    [Google Scholar]
  17. Bergmann JS, Keating WE, Woods GL. Clinical evaluation of the BDProbeTec ET system for rapid detection of Mycobacterium tuberculosis . J Clin Microbiol 2000; 38:863–865
    [Google Scholar]
  18. Somu N, Swaminathan S, Paramasivan CN. et al. Value of bronchoalveolar lavage and gastric lavage in the diagnosis of pulmonary tuberculosis in children. Tubercle Lung Dis 1995; 76:295–299 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/0022-1317-51-10-895
Loading
/content/journal/jmm/10.1099/0022-1317-51-10-895
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error