1887

Abstract

Three serovars (5, 8 and 10) of were inoculated intravaginally into groups of oestradiol-treated young adult BALB/c strain mice. Hormone treatment was essential for vaginal colonisation. The proportion of mice colonised initially and the persistence of colonisation were different with the three serovars; half of those given serovar 8 were still colonised after 84 days. A strain of serovar 5 after a further 50 subcultures was a little less persistent than it was before such subculture, but not in a way to suggest that subculturing was the main reason for differences in the behaviour of the serovars. At autopsy of six mice that were still colonised vaginally 158 days after inoculation of serovar 8, spread to the upper genital tract was shown to have occurred in three of them and dissemination to the liver and kidney in one. Compared with immunocompetent mice of strain CB20, such dissemination was not a feature in genetically related mice with severe combined immunodeficiency. This is not in keeping with the situation in hypogammaglobulinaemic patients in whom ureaplasmas and other mycoplasmas are known to disseminate. However, differences in the proportion of immunocompetent mice colonised or in ureaplasmal persistence with different serovars may act as a marker for differences in human pathogenicity and is worthy of further study.

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2002-10-01
2024-12-07
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References

  1. Taylor-Robinson D, Tully JG. Mycoplasmas, ureaplasmas, spiroplasmas and related organisms. In Collier L, Sussman M, Balows A. eds Topley & Wilson's microbiology and microbial infections. , 9th edn. Vol 2 Systematic bacteriology (Balows A, Duerden BI London, Arnold: 1998799–827
    [Google Scholar]
  2. Taylor-Robinson D, Furr PM. Update on sexually transmitted mycoplasmas. Lancet 1998; 351 Suppl 3:12–15 [CrossRef]
    [Google Scholar]
  3. Furr PM, Taylor-Robinson D. The establishment and persistence of Ureaplasma urealyticum in oestradiol-treated female mice. J Med Microbiol 1989; 29:111–114 [CrossRef]
    [Google Scholar]
  4. Taylor-Robinson D, Martin-Bourgon C, Watanabe T, Addey JP. Isolation of T-mycoplasmas from dogs and squirrel monkeys: biological and serological comparison with those isolated from man and cattle. J Gen Microbiol 1971; 68:97–107 [CrossRef]
    [Google Scholar]
  5. Taylor-Robinson D, Csonka GW, Prentice MJ. Human intra-urethral inoculation of ureaplasmas. Q J Med 1977; 46:309–326
    [Google Scholar]
  6. Rugh R. The mouse. Its reproduction and development. Minneapolis, Burgess Publishing 196838–39
    [Google Scholar]
  7. Manchee RJ, Taylor-Robinson D. Studies on the nature of receptors involved in attachment of tissue culture cells to mycoplasmas. Br J Exp Pathol 1969; 50:66–75
    [Google Scholar]
  8. Smith PF. Mass cultivation of ureaplasmas and some applications. Pediatr Infect Dis 1986; 5: 6 SupplS313–S315 [CrossRef]
    [Google Scholar]
  9. Furr PM, Sarathchandra P, Hetherington CM, Taylor-Robinson D. Site of localization of Mycoplasma pulmonis and Mycoplasma hominis in the genital tract of female mice demonstrated by culture and scanning and immuno-electron microscopy. Int J Exp Pathol 1995; 76:131–143
    [Google Scholar]
  10. Furr PM, Taylor-Robinson D. The influence of hormones on the bacterial flora of the murine vagina and its implications for human disease. Microb Ecol Hlth Dis 1991; 4:141–148 [CrossRef]
    [Google Scholar]
  11. Furr PM, Taylor-Robinson D. Oestradiol-induced infection of the genital tract of female mice by Mycoplasma hominis . J Gen Microbiol 1989; 135:2743–2749
    [Google Scholar]
  12. Taylor-Robinson D, Furr PM. Observations on the antibiotic treatment of experimentally induced mycoplasmal infections in mice. J Antimicrob Chemother 2000; 45:903–907 [CrossRef]
    [Google Scholar]
  13. Webster ADB, Furr PM, Hughes-Jones NC, Gorick BD, Taylor-Robinson D. Critical dependence on antibody for defence against mycoplasmas. Clin Exp Immunol 1988; 71:383–387
    [Google Scholar]
  14. Furr PM, Taylor-Robinson D, Webster ADB. Mycoplasmas and ureaplasmas in patients with hypogammaglobulinaemia and their role in arthritis: microbiological observations over twenty years. Ann Rheum Dis 1994; 53:183–187 [CrossRef]
    [Google Scholar]
  15. Taylor-Robinson D, Furr PM, Hetherington CM. Neisseria gonorrhoeae colonises the genital tract of oestradiol-treated germ-free female mice. Microb Pathog 1990; 9:369–374 [CrossRef]
    [Google Scholar]
  16. Povlsen K, Bjørnelius E, Lidbrink P, Lind I. Relationship of Ureaplasma urealyticum biovar 2 to nongonococcal urethritis. Eur J Clin Microbiol Infect Dis 2002; 21:97–101 [CrossRef]
    [Google Scholar]
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