1887

Abstract

Data relating to hantavirus infection in Spain are scarce and limited to rural areas. The aim of this work was to study the seroprevalence of hantavirus infection in the Autonomous Community of Madrid (ACM), a region containing both rural and urban populations in different ecological settings. Sera from 3852 individuals (1849 male, 2003 female) were screened by indirect inmunofluorescence, with Vero E6 cells infected with Puumala, Hantaan and Seoul viruses as antigens. Screen-positive results were confirmed by Western blot with recombinant Seoul virus nucleocapsid protein as antigen. Antibodies against hantavirus were detected in 12 sera (0.31%). No statistical differences were found according to sex and age. The highest prevalence was found in the south-eastern area, significantly higher than the central and north-western areas. The most frequent serological pattern was reactivity against all three viruses used (33.3% of all positive sera). Therefore, this study confirms the presence of hantavirus infection in the ACM, including for the first time an urban area of Spain, but with the highest prevalence in a rural area. Serological evidence suggests that there is more than one circulating serotype.

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2002-10-01
2019-11-21
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References

  1. Hjelle B, Jenison SA, Goade DE, Green WB, Feddersen RM, Scott AA. Hantaviruses: clinical, microbiologic and epidemiologic aspects. Crit Rev Clin Lab Sci 1995;; 32: 469–508.[CrossRef]
    [Google Scholar]
  2. Nowotny N. The domestic cat: a possible transmitter of viruses from rodents to man. Lancet 1994;; 343: 921.
    [Google Scholar]
  3. Kim GR, Lee YT, Park CH. A new natural reservoir of hantavirus: isolation of hantaviruses from lung tissues of bats. Arch Virol 1994;; 134: 85–95.[CrossRef]
    [Google Scholar]
  4. Slonova RA, Tkachenko EA, Kushnarev EL, Dzagurova TK, Astakova TI. Hantavirus isolation from birds. Acta Virol 1992;; 36: 493.
    [Google Scholar]
  5. Schlmaljohn CS, Dalrymple JM. Hantaviruses. In: Webster RG, Granoff A (eds) Encyclopedia of virology, vol 2.London, Academic Press. 1994:; 538–545.
  6. Gegúndez MI, Saz JV, Alves MJ, Merino FJ, Filipe AR, Beltrán M. Infección por Hantavirus en España: Estudio seroepidemiológico en la provincia de Soria.[Hantavirus infection in Spain: seroepidemiologic study in the province of Soria.] Med Clin 1996;; 106: 131–133.
    [Google Scholar]
  7. Rodríguez JA, Vaqué J. La enfermedad por Hantavirus: una infección emergente.[Hantavirus disease: an emerging infection.] Enferm Infecc Microbiol Clin 1994;; 12: 477–479.
    [Google Scholar]
  8. Salavert M, López-Hontangas JL, Ponz F, Gobernado M. Infección por Hantavirus y síndrome pulmonar, otra nueva entidad que buscar en nuestro entorno? [Hantavirus infection and the pulmonary syndrome: another new entity to look for in our environment?] Enferm Infecc Microbiol Clin 1996;; 14: 395–396.
    [Google Scholar]
  9. Pérez-Molina JA, Escudero Nieto R, Settergren B, Guerrero Espejo A. Debemos buscar la infección por Hantavirus en pacientes españoles? [Should we look for Hantavirus infection in Spanish patients?] Enferm Infecc Microbiol Clin 1995;; 13: 125–126.
    [Google Scholar]
  10. Lee HW, Lee PW. Korean hemorrhagic fever I.Demonstration of causative antigen and antibodies. Korean J Intern Med 1976;; 19: 371–383.
    [Google Scholar]
  11. Jenison S, Yamada T, Morris C.et al. Characterization of human antibody responses to Four Corners Hantavirus infection among patients with Hantavirus pulmonary syndrome. J Virol 1994;; 68: 3000–3006.
    [Google Scholar]
  12. Nuti M, Amaddeo D, Autorino GL.et al. Seroprevalence of antibodies to hantaviruses and leptospires in selected Italian population groups. Eur J Epidemiol 1992;; 8: 98–102.
    [Google Scholar]
  13. Groen J, Gerding MN, Jordans JG, Clement JP, Nieuwenhuijs JHM, Osterhaus ADME. Hantavirus infections in The Netherlands: epidemiology and disease. Epidemiol Infect 1995;; 114: 373–383.[CrossRef]
    [Google Scholar]
  14. Zollër L, Faulde M, Meisel H.et al. Seroprevalence of hantavirus antibodies in Germany as determined by a new recombinant enzyme immunoassay. Eur J Clin Microbiol Infect Dis 1995;; 14: 305–313.[CrossRef]
    [Google Scholar]
  15. Papadimitriou MG, Antoniadis A. Hantavirus nephropathy in Greece. Lancet 1994;; 343: 1038.
    [Google Scholar]
  16. Brummer-Kurvenkontio M, Vapalahti O, Henttonen H, Koskela P, Kuusisto P, Vaheri A. Epidemiological study of Nephropathia epidemica in Finland 1989–96. Scand J Infect Dis 1999;; 31: 427–435.[CrossRef]
    [Google Scholar]
  17. Saz JV, Gegúndez MI, Beltrán M. Hantavirus. Zamora, Junta de Castilla y León, Consejeria de Sanidad y Bienestar Social. 1997
  18. Mustonen J, Vaheri A, Clement J. Third International Conference on Haemorrhagic Fever with Renal Syndrome (HFRS) and Hantaviruses. Nephrol Dial Transplant 1996;; 11: 730–733.[CrossRef]
    [Google Scholar]
  19. Ahlm C, Linderholm M, Juto P, Stegmayr B, Settergren B. Prevalence of serum IgG antibodies to Puumala virus (haemorrhagic fever with renal syndrome) in Northern Sweden. Epidemiol Infect 1994;; 113: 129–136.[CrossRef]
    [Google Scholar]
  20. Niklasson B, Hornfeldt B, Mullaart M.et al. An epidemiologic study of hemorrhagic fever with renal syndrome in Bashkirtostan (Russian) and Sweden. Am J Trop Hyg 1993;; 48: 670–675.
    [Google Scholar]
  21. Settergren B, Ahlm C, Juto P, Niklasson B. Specific Puumala virus IgG half a century after haemorrhagic fever with renal syndrome. Lancet 1991;; 338: 66.
    [Google Scholar]
  22. Vapalahti O, Kallio-Kokko H, Närvänen A.et al. Human B-cell epitopes of Puumala virus nucleocapsid protein, the major antigen in early serological response. J Med Virol 1995;; 46: 293–303.[CrossRef]
    [Google Scholar]
  23. Salonen E-M, Parren PWHI, Graus YF.et al. Human recombinant Puumala virus antibodies: cross-reaction with other hantaviruses and use in diagnostics. J Gen Virol 1998;; 79: 659–665.
    [Google Scholar]
  24. Castell A, Mayo M. Guía de los mamíferos en libertad en España y Portugal. Madrid, Editorial Pirámide. 1993:; 157–160.
  25. McKenna P, Clement J, Matthys P, Coyle PV, McCaughey C. Serological evidence of hantavirus disease in Northern Ireland. J Med Virol 1994;; 43: 33–38.[CrossRef]
    [Google Scholar]
  26. Filipe AR, Andrade HR, Sommer AI, Traavik T. Hantaviral antigens and antibodies in wild rodents in Portugal. Acta Virol 1991;; 35: 287–291.
    [Google Scholar]
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