@article{mbs:/content/journal/jmm/10.1099/0022-1317-50-6-571, author = "LE, C.T. and GRAY, G.C. and PODDAR, S.K.", title = "A modified rapid method of nucleic acid isolation from suspension of matured virus: applied in restriction analysis of DNA from an adenovirus prototype strain and a patient isolate", journal= "Journal of Medical Microbiology", year = "2001", volume = "50", number = "6", pages = "571-574", doi = "https://doi.org/10.1099/0022-1317-50-6-571", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/0022-1317-50-6-571", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = " This report describes a method for the isolation of nucleic acid from a suspension of matured virus. Nucleic acid (DNA) was isolated from a prototype strain of adenovirus type 7 and a clinical isolate of adenovirus type 7. Instead of the usual method of ultracentifugation, a filtration method was applied to concentrate the virus rapidly and nucleic acid was then isolated by a standard phenol/chloroform/isoamyl-alcohol extraction procedure. The DNA was found to be sufficiently purified to generate a reproducible restriction endonuclease digestion pattern. The clinical isolate of adenovirus type 7 revealed loss of restriction site for the endonuclease HindIII when compared with the prototype strain. ", }